Reviewed, UniProtKB/Swiss-Prot Q6FCT2 (SYA_ACIAD)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name(s)=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	ACIAD1253

Organism

Acinetobacter sp. (strain ADP1) [Complete proteome] [HAMAP]

Taxonomic identifier

62977 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	878 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Domain	Zinc-finger
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 878	878	Alanyl-tRNA synthetase
Regions
	Zinc finger	177 – 190	14	C2H2-type

Sequences

	Sequence		Length	Mass (Da)
	Q6FCT2-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 2594DCD48229355A	FASTA	878	96,691
	10 20 30 40 50 60 MTSAEIREAF LRYFESQGHT RVASSSLVPA NDPTLLFTNA GMNQFKDCFL GLEKRDYVRA 70 80 90 100 110 120 TTSQKCVRAG GKHNDLDNVG YTARHHTFFE MLGNFSFGDY FKRDALTFAW DFLTSEQWLG 130 140 150 160 170 180 LPKDKLYVTV YHTDDEAYDI WNKEIGLAAD RIIRIGDNKG EKYASDNFWA MGDTGPCGPC 190 200 210 220 230 240 SEIFFDHGDH IWGGLPGTPE EDGDRFIEIW NNVFMQFNRT ADGVLHPLPA PSVDTGMGLE 250 260 270 280 290 300 RISAVLQHVN SNYDIDLFQH LIKSACEIIN VKDEGQPSLR VVADHARSCC FLIADGVNPS 310 320 330 340 350 360 NEGRGYVLRR IIRRAVRHGN KLGATGTFFY KMLQPLIDVM GDAYPELKND QARIEATLIR 370 380 390 400 410 420 EEEQFAKTLE QGLKLLEGEL TQLSGKVIPG ETVFKLYDTY GFPTDLTADI ARERDLEIDE 430 440 450 460 470 480 VGFEREMEAQ RRRARDAGKF AVDYNSIVKV EGETQFDGYH ATAGQGEIVA IYKDGEQVDE 490 500 510 520 530 540 VVEGDEALIV LNQTPFYAES GGQIGDTGLF KNDTGIFEVQ DTKKSGGAFV HQGIVTMGSL 550 560 570 580 590 600 KAAQHVDAIV KADIRAATAR NHSATHLLHA ALRQILGTHV QQKGSLVASD ILRFDFANDQ 610 620 630 640 650 660 PVSFEQLQEI ERLVNAEVIA NSAVTTELLD IESAKAKGAM MLFGEKYGDE VRVLSMGSVI 670 680 690 700 710 720 DEHNFSIELC GGIHVQRTGD IGLFKIISEG GVAAGIRRIE AVTGTKALEV VQKADADIQH 730 740 750 760 770 780 INSLLKAQKD QTVERVQAAV EQTSALHKQI EQLNQKLANF QAAELLSQVQ NIAGRSTLIT 790 800 810 820 830 840 TVQNIDAKSL RNLHDSVKSK LEDAVIVLAG LEGDKISLIA SVAKQYTAHL KAGDIIKHLA 850 860 870 TELGGKGGGK PDLAQGGAPL NEKFEHVIAA LPAWLEQH

References

[1]

"Unique features revealed by the genome sequence of Acinetobacter sp. ADP1, a versatile and naturally transformation competent bacterium." Barbe V., Vallenet D., Fonknechten N., Kreimeyer A., Oztas S., Labarre L., Cruveiller S., Robert C., Duprat S., Wincker P., Ornston L.N., Weissenbach J., Marliere P., Cohen G.N., Medigue C. Nucleic Acids Res. 32:5766-5779(2004) [PubMed: 15514110] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR543861 Genomic DNA. Translation: CAG68127.1.
RefSeq	YP_045949.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2878212.
GenomeReviews	Gene locus ACIAD1253 in contig CR543861_GR.
KEGG	aci:ACIAD1253.
NMPDR	fig|62977.3.peg.322.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6FCT2.
Enzyme and pathway databases
BioCyc	ASP62977:ACIAD1253-MON.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProDom	Q6FCT2. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

SYA_ACIAD

Accession

Primary (citable) accession number: Q6FCT2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 15, 2005
Last sequence update:	July 19, 2004
Last modified:	July 22, 2008
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)