Actin-binding LIM protein 2 - Homo sapiens (Human)

Names and origin

Protein names

Recommended name:
    Actin-binding LIM protein 2
Alternative name(s):
    Actin-binding LIM protein family member 2
      Short name=abLIM-2

Gene names

Name:	ABLIM2
Synonyms:	KIAA1808

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	611 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	May act as scaffold protein. May stimulate ABRA activity and ABRA-dependent SRF transcriptional activity.
Subunit structure	Interacts with F-actin and ABRA.
Subcellular location	Cytoplasm. Note= In skeletal muscle, sarcomeric or cosarcomeric localization By similarity.
Tissue specificity	Highly expressed in skeletal muscle.
Sequence similarities	Contains 1 HP (headpiece) domain. Contains 4 LIM zinc-binding domains.

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Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Domain	LIM domain Repeat
Ligand	Metal-binding Zinc
PTM	Phosphoprotein
Technical term	3D-structure
Gene Ontology (GO)
Cellular component	cytoplasm Non-traceable author statement. Source: UniProtKB
Molecular function	zinc ion binding Non-traceable author statement. Source: UniProtKB
Complete GO annotation...

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Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6H8Q1-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6H8Q1-2) The sequence of this isoform differs from the canonical sequence as follows: 506-545: RFPYSKSDPLPGHGKNGLDQRNANLAPCGADPDASWGMRE → Q

Isoform 3 (identifier: Q6H8Q1-3) The sequence of this isoform differs from the canonical sequence as follows: 349-349: E → ESPQLLSPTPTE 390-441: Missing. 506-545: RFPYSKSDPLPGHGKNGLDQRNANLAPCGADPDASWGMRE → Q

Isoform 4 (identifier: Q6H8Q1-4) The sequence of this isoform differs from the canonical sequence as follows: 1-243: Missing. 349-349: E → ESPQLLSPTPTE 389-422: Missing. 459-459: K → KQ
Notes: No experimental confirmation available.

Isoform 5 (identifier: Q6H8Q1-5) The sequence of this isoform differs from the canonical sequence as follows: 459-459: K → KQ 506-545: RFPYSKSDPLPGHGKNGLDQRNANLAPCGADPDASWGMRE → Q
Notes: No experimental confirmation available.

Isoform 6 (identifier: Q6H8Q1-6) The sequence of this isoform differs from the canonical sequence as follows: 349-349: E → ESPQLLSPTPTE 390-441: Missing. 459-459: K → KQ 507-510: FPYS → EWFF 511-611: Missing.
Notes: No experimental confirmation available.

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Molecule processing

Chain

1 – 611

611

Actin-binding LIM protein 2

Regions

Domain

22 – 81

60

LIM zinc-binding 1

Domain

81 – 141

61

LIM zinc-binding 2

Domain

151 – 210

60

LIM zinc-binding 3

Domain

210 – 270

61

LIM zinc-binding 4

Domain

543 – 611

69

HP

Amino acid modifications

Modified residue

289

1

Phosphoserine By similarity

Modified residue

294

1

Phosphoserine By similarity

Modified residue

296

1

Phosphoserine By similarity

Modified residue

476

1

Phosphoserine By similarity

Natural variations

Alternative sequence

1 – 243

243

Missing in isoform 4.

Alternative sequence

349

1

E → ESPQLLSPTPTE in isoform 3, isoform 4 and isoform 6.

Alternative sequence

389 – 422

34

Missing in isoform 4.

Alternative sequence

390 – 441

52

Missing in isoform 3 and isoform 6.

Alternative sequence

459

1

K → KQ in isoform 4, isoform 5 and isoform 6.

Alternative sequence

506 – 545

40

RFPYS…WGMRE → Q in isoform 2, isoform 3 and isoform 5.

Alternative sequence

507 – 510

4

FPYS → EWFF in isoform 6.

Alternative sequence

511 – 611

101

Missing in isoform 6.

Experimental info

Sequence conflict

240

1

R → G in BAC04414. Ref.2

Sequence conflict

312

1

D → G in BAC04414. Ref.2

Secondary structure

1

.

611

Helix Strand Turn

Details...

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Sequences

Sequence		Length	Mass (Da)
Isoform 1 [UniParc]. Last modified December 7, 2004. Version 2. Checksum: 225A4E25646B370B Show »	FASTA	611	67,812
10 20 30 40 50 60 MSAVSQPQAA PSPLEKSPST AILCNTCGNV CKGEVLRVQD KYFHIKCFVC KACGCDLAEG 70 80 90 100 110 120 GFFVRQGEYI CTLDYQRLYG TRCFSCDQFI EGEVVSALGK TYHPDCFVCA VCRLPFPPGD 130 140 150 160 170 180 RVTFNGKECM CQKCSLPVSV GSSAHLSQGL RSCGGCGTEI KNGQALVALD KHWHLGCFKC 190 200 210 220 230 240 KSCGKLLNAE YISKDGLPYC EADYHAKFGI RCDSCEKYIT GRVLEAGEKH YHPSCALCVR 250 260 270 280 290 300 CGQMFAEGEE MYLQGSSIWH PACRQAARTE DRNKETRTSS ESIISVPASS TSGSPSRVIY 310 320 330 340 350 360 AKLGGEILDY RDLAALPKSK AIYDIDRPDM ISYSPYISHS AGDRQSYGEG DQDDRSYKQC 370 380 390 400 410 420 RTSSPSSTGS VSLGRYTPTS RSPQHYSRPG SESGRSTPSL SVLSDSKPPP STYQQAPRHF 430 440 450 460 470 480 HVPDTGVKDN IYRKPPIYRQ HAARRSDGED GSLDQDNRKK SSWLMLKGDA DTRTNSPDLD 490 500 510 520 530 540 TQSLSHSSGT DRDPLQRMAG DSFHSRFPYS KSDPLPGHGK NGLDQRNANL APCGADPDAS 550 560 570 580 590 600 WGMREYKIYP YDSLIVTNRI RVKLPKDVDR TRLERHLSPE EFQEVFGMSI EEFDRLALWK 610 RNDLKKKALL F « Hide
Isoform 2 [UniParc]. Checksum: F2CF3EEE6B1CCADC Show »		572	63,632
10 20 30 40 50 60 MSAVSQPQAA PSPLEKSPST AILCNTCGNV CKGEVLRVQD KYFHIKCFVC KACGCDLAEG 70 80 90 100 110 120 GFFVRQGEYI CTLDYQRLYG TRCFSCDQFI EGEVVSALGK TYHPDCFVCA VCRLPFPPGD 130 140 150 160 170 180 RVTFNGKECM CQKCSLPVSV GSSAHLSQGL RSCGGCGTEI KNGQALVALD KHWHLGCFKC 190 200 210 220 230 240 KSCGKLLNAE YISKDGLPYC EADYHAKFGI RCDSCEKYIT GRVLEAGEKH YHPSCALCVR 250 260 270 280 290 300 CGQMFAEGEE MYLQGSSIWH PACRQAARTE DRNKETRTSS ESIISVPASS TSGSPSRVIY 310 320 330 340 350 360 AKLGGEILDY RDLAALPKSK AIYDIDRPDM ISYSPYISHS AGDRQSYGEG DQDDRSYKQC 370 380 390 400 410 420 RTSSPSSTGS VSLGRYTPTS RSPQHYSRPG SESGRSTPSL SVLSDSKPPP STYQQAPRHF 430 440 450 460 470 480 HVPDTGVKDN IYRKPPIYRQ HAARRSDGED GSLDQDNRKK SSWLMLKGDA DTRTNSPDLD 490 500 510 520 530 540 TQSLSHSSGT DRDPLQRMAG DSFHSQYKIY PYDSLIVTNR IRVKLPKDVD RTRLERHLSP 550 560 570 EEFQEVFGMS IEEFDRLALW KRNDLKKKAL LF « Hide
Isoform 3 [UniParc]. Checksum: 1E6B36B85E5176D8 Show »		531	58,984
10 20 30 40 50 60 MSAVSQPQAA PSPLEKSPST AILCNTCGNV CKGEVLRVQD KYFHIKCFVC KACGCDLAEG 70 80 90 100 110 120 GFFVRQGEYI CTLDYQRLYG TRCFSCDQFI EGEVVSALGK TYHPDCFVCA VCRLPFPPGD 130 140 150 160 170 180 RVTFNGKECM CQKCSLPVSV GSSAHLSQGL RSCGGCGTEI KNGQALVALD KHWHLGCFKC 190 200 210 220 230 240 KSCGKLLNAE YISKDGLPYC EADYHAKFGI RCDSCEKYIT GRVLEAGEKH YHPSCALCVR 250 260 270 280 290 300 CGQMFAEGEE MYLQGSSIWH PACRQAARTE DRNKETRTSS ESIISVPASS TSGSPSRVIY 310 320 330 340 350 360 AKLGGEILDY RDLAALPKSK AIYDIDRPDM ISYSPYISHS AGDRQSYGES PQLLSPTPTE 370 380 390 400 410 420 GDQDDRSYKQ CRTSSPSSTG SVSLGRYTPT SRSPQHYSRP AARRSDGEDG SLDQDNRKKS 430 440 450 460 470 480 SWLMLKGDAD TRTNSPDLDT QSLSHSSGTD RDPLQRMAGD SFHSQYKIYP YDSLIVTNRI 490 500 510 520 530 RVKLPKDVDR TRLERHLSPE EFQEVFGMSI EEFDRLALWK RNDLKKKALL F « Hide
Isoform 4 [UniParc]. Checksum: 00733A9BC0EA7BE5 Show »		346	38,966
10 20 30 40 50 60 MFAEGEEMYL QGSSIWHPAC RQAARTEDRN KETRTSSESI ISVPASSTSG SPSRVIYAKL 70 80 90 100 110 120 GGEILDYRDL AALPKSKAIY DIDRPDMISY SPYISHSAGD RQSYGESPQL LSPTPTEGDQ 130 140 150 160 170 180 DDRSYKQCRT SSPSSTGSVS LGRYTPTSRS PQHYSRPDTG VKDNIYRKPP IYRQHAARRS 190 200 210 220 230 240 DGEDGSLDQD NRKQKSSWLM LKGDADTRTN SPDLDTQSLS HSSGTDRDPL QRMAGDSFHS 250 260 270 280 290 300 RFPYSKSDPL PGHGKNGLDQ RNANLAPCGA DPDASWGMRE YKIYPYDSLI VTNRIRVKLP 310 320 330 340 KDVDRTRLER HLSPEEFQEV FGMSIEEFDR LALWKRNDLK KKALLF « Hide
Isoform 5 [UniParc]. Checksum: DFD6CB38CF846982 Show »		573	63,760
10 20 30 40 50 60 MSAVSQPQAA PSPLEKSPST AILCNTCGNV CKGEVLRVQD KYFHIKCFVC KACGCDLAEG 70 80 90 100 110 120 GFFVRQGEYI CTLDYQRLYG TRCFSCDQFI EGEVVSALGK TYHPDCFVCA VCRLPFPPGD 130 140 150 160 170 180 RVTFNGKECM CQKCSLPVSV GSSAHLSQGL RSCGGCGTEI KNGQALVALD KHWHLGCFKC 190 200 210 220 230 240 KSCGKLLNAE YISKDGLPYC EADYHAKFGI RCDSCEKYIT GRVLEAGEKH YHPSCALCVR 250 260 270 280 290 300 CGQMFAEGEE MYLQGSSIWH PACRQAARTE DRNKETRTSS ESIISVPASS TSGSPSRVIY 310 320 330 340 350 360 AKLGGEILDY RDLAALPKSK AIYDIDRPDM ISYSPYISHS AGDRQSYGEG DQDDRSYKQC 370 380 390 400 410 420 RTSSPSSTGS VSLGRYTPTS RSPQHYSRPG SESGRSTPSL SVLSDSKPPP STYQQAPRHF 430 440 450 460 470 480 HVPDTGVKDN IYRKPPIYRQ HAARRSDGED GSLDQDNRKQ KSSWLMLKGD ADTRTNSPDL 490 500 510 520 530 540 DTQSLSHSSG TDRDPLQRMA GDSFHSQYKI YPYDSLIVTN RIRVKLPKDV DRTRLERHLS 550 560 570 PEEFQEVFGM SIEEFDRLAL WKRNDLKKKA LLF « Hide
Isoform 6 [UniParc]. Checksum: FD93E620E09C0742 Show »		470	51,698
10 20 30 40 50 60 MSAVSQPQAA PSPLEKSPST AILCNTCGNV CKGEVLRVQD KYFHIKCFVC KACGCDLAEG 70 80 90 100 110 120 GFFVRQGEYI CTLDYQRLYG TRCFSCDQFI EGEVVSALGK TYHPDCFVCA VCRLPFPPGD 130 140 150 160 170 180 RVTFNGKECM CQKCSLPVSV GSSAHLSQGL RSCGGCGTEI KNGQALVALD KHWHLGCFKC 190 200 210 220 230 240 KSCGKLLNAE YISKDGLPYC EADYHAKFGI RCDSCEKYIT GRVLEAGEKH YHPSCALCVR 250 260 270 280 290 300 CGQMFAEGEE MYLQGSSIWH PACRQAARTE DRNKETRTSS ESIISVPASS TSGSPSRVIY 310 320 330 340 350 360 AKLGGEILDY RDLAALPKSK AIYDIDRPDM ISYSPYISHS AGDRQSYGES PQLLSPTPTE 370 380 390 400 410 420 GDQDDRSYKQ CRTSSPSSTG SVSLGRYTPT SRSPQHYSRP AARRSDGEDG SLDQDNRKQK 430 440 450 460 470 SSWLMLKGDA DTRTNSPDLD TQSLSHSSGT DRDPLQRMAG DSFHSREWFF « Hide

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References

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[1]	"Structure and transcriptional activity of the ABLIM2 gene of human, mouse and rat." Klimov E.A., Rakhmanaliev E.R., Rudco O.I. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3 AND 4). Tissue: Brain.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 6). Tissue: PNS.
[4]	"Prediction of the coding sequences of unidentified human genes. XX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Nakayama M., Nakajima D., Kikuno R., Ohara O. DNA Res. 8:85-95(2001) [PubMed: 11347906] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 34-611 (ISOFORM 2). Tissue: Brain.
[5]	The German cDNA consortium Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 390-572 (ISOFORM 5). Tissue: Amygdala.
[6]	"Solution structure of the LIM domain of the human actin binding LIM protein 2." RIKEN structural genomics initiative (RSGI) Submitted (JUN-2004) to the PDB data bank Cited for: STRUCTURE BY NMR OF 73-140.
[7]	"Two novel members of the ABLIM protein family, ABLIM-2 and -3, associate with STARS and directly bind F-actin." Barrientos T., Frank D., Kuwahara K., Bezprozvannaya S., Pipes G.C.T., Bassel-Duby R., Richardson J.A., Katus H.A., Olson E.N., Frey N. J. Biol. Chem. 282:8393-8403(2007) [PubMed: 17194709] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, INTERACTION WITH ABRA.