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Reviewed, UniProtKB/Swiss-Prot Q6NS38 (ALKB2_HUMAN)

Last modified September 2, 2008. Version 37. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2
    EC=1.14.11.-
Alternative name(s):
    Alkylated DNA repair protein alkB homolog 2
    Oxy DC1
Gene names
Name: ALKBH2
Synonyms: ABH2
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length261 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Dioxygenase that repairs alkylated DNA and RNA containing 1-methyladenine and 3-methylcytosine by oxidative demethylation. Has strong preference for double-stranded DNA. Has low efficiency with single-stranded substrates. Requires molecular oxygen, alpha-ketoglutarate and iron.

Cofactor

Binds 1 Fe(2+) ion per subunit.

Enzyme regulation

Activated by ascorbate.

Subcellular location

Nucleus. Note= Detected in replication foci during S-phase.

Tissue specificity

Detected in colon, small intestine, ovary, testis, prostate, skeletal muscle, heart, liver and urinary bladder.

Sequence similarities

Belongs to the alkB family.

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Ontologies

Keywords

   Biological processDNA damage
DNA repair
   Cellular componentNucleus
   LigandIron
Metal-binding
   Molecular functionDioxygenase
Oxidoreductase
   Technical term3D-structure

Gene Ontology (GO)

   Cellular componentnucleoplasm Ref.3

Inferred from Experiment. Source: Reactome

   Molecular functiondamaged DNA binding Ref.3

Inferred from Experiment. Source: Reactome

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical view

Molecule processing

Chain1 – 261261Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2

Regions

Region122 – 1243Substrate binding By similarity
Region248 – 2547Alpha-ketoglutarate binding By similarity

Sites

Metal binding1711Iron; catalytic By similarity
Metal binding1731Iron; catalytic By similarity
Metal binding2361Iron; catalytic By similarity
Binding site911Substrate By similarity
Binding site1101Substrate Potential
Binding site1591Alpha-ketoglutarate By similarity
Binding site1611Alpha-ketoglutarate By similarity
Binding site1741Substrate By similarity

Experimental info

Mutagenesis1731D → A: Loss of activity
Mutagenesis2361H → A: Reduced activity
Sequence conflict431R → G in AAV28301. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
Q6NS38-1 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: A376E13F92621A01

FASTA26129,322
        10         20         30         40         50         60 
MDRFLVKGAQ GGLLRKQEEQ EPTGEEPAVL GGDKESTRKR PRREAPGNGG HSAGPSWRHI 

        70         80         90        100        110        120 
RAEGLDCSYT VLFGKAEADE IFQELEKEVE YFTGALARVQ VFGKWHSVPR KQATYGDAGL 

       130        140        150        160        170        180 
TYTFSGLTLS PKPWIPVLER IRDHVSGVTG QTFNFVLINR YKDGCDHIGE HRDDERELAP 

       190        200        210        220        230        240 
GSPIASVSFG ACRDFVFRHK DSRGKSPSRR VAVVRLPLAH GSLLMMNHPT NTHWYHSLPV 

       250        260 
RKKVLAPRVN LTFRKILLTK K

« Hide

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References

« Hide 'large scale' references
[1]"Cloning and expression of human 2OG-Fe(II) oxy DC1."
Lin Y., Xie Y., Mao Y.
Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Skin.
[3]"Reversal of DNA alkylation damage by two human dioxygenases."
Duncan T., Trewick S.C., Koivisto P., Bates P.A., Lindahl T., Sedgwick B.
Proc. Natl. Acad. Sci. U.S.A. 99:16660-16665(2002) [PubMed: 12486230] [Abstract]
Cited for: FUNCTION, ENZYME REGULATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[4]"Human and bacterial oxidative demethylases repair alkylation damage in both RNA and DNA."
Aas P.A., Otterlei M., Falnes P.O., Vaagboe C.B., Skorpen F., Akbari M., Sundheim O., Bjoeraas M., Slupphaug G., Seeberg E., Krokan H.E.
Nature 421:859-863(2003) [PubMed: 12594517] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[5]"Repair of methylation damage in DNA and RNA by mammalian AlkB homologues."
Lee D.-H., Jin S.-G., Cai S., Chen Y., Pfeifer G.P., O'Connor T.R.
J. Biol. Chem. 280:39448-39459(2005) [PubMed: 16174769] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF ASP-173 AND HIS-236, TISSUE SPECIFICITY.

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Cross-references

Sequence databases

AY754389 mRNA. Translation: AAV28301.1.
BC070489 mRNA. Translation: AAH70489.1.
RefSeqNP_001001655.1.
UniGeneHs.374458

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
3BTYX-ray2.35A56-258[»]
3BTZX-ray3.00A57-258[»]
3BU0X-ray2.50A56-258[»]
3BUCX-ray2.59A56-258[»]
ModBaseSearch...

Genome annotation databases

EnsemblENSG00000189046. Homo sapiens. [Contig view]
GeneID121642.
KEGGhsa:121642.

Organism-specific databases

HGNCHGNC:32487. ALKBH2.
HPACAB011198.
MIM610602. gene.
GenAtlasSearch...
GeneCardsSearch...

Phylogenomic databases

HOGENOMQ6NS38.
HOVERGENQ6NS38.

Enzyme and pathway databases

ReactomeREACT_216. DNA Repair.

Gene expression databases

ArrayExpressQ6NS38.
CleanExHS_ALKBH2.
GermOnlineENSG00000189046. Homo sapiens.

Family and domain databases

InterProIPR005123. 2OG-FeII_Oase.
[Graphical view]
PfamPF03171. 2OG-FeII_Oxy. 1 hit.
[Graphical view]
ProDomQ6NS38.
[Graphical view] [Entries sharing at least one domain]
BLOCKSSearch...

Other Resources

DrugBankDB00126. Vitamin C.
SOURCESearch...
ProtoNetSearch...

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Entry information

Entry nameALKB2_HUMAN
AccessionPrimary (citable) accession number: Q6NS38
Secondary accession number(s): Q5XLE3
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: July 5, 2004
Last modified: September 2, 2008
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

Human chromosome 12

Human chromosome 12: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

UniProtKB secondary accession numbers

Index of UniProtKB secondary accession numbers

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents