Reviewed, UniProtKB/Swiss-Prot Q70KF8 (DHSB_UROFA)

Names and origin

Protein names

Recommended name:
    Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial
    EC=1.3.5.1
Alternative name(s):
    Iron-sulfur subunit of complex II
      Short name=Ip

Gene names

Name:	SDH2
Synonyms:	sucDH1

Organism

Uromyces fabae (Rust fungus)

Taxonomic identifier

55588 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Pucciniomycotina › Pucciniomycetes › Pucciniales › Pucciniaceae › Uromyces

Protein attributes

Sequence length	283 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) By similarity.
Catalytic activity	Succinate + ubiquinone = fumarate + ubiquinol.
Cofactor	Binds 1 2Fe-2S cluster By similarity. Binds 1 3Fe-4S cluster By similarity. Binds 1 4Fe-4S cluster By similarity.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle.
Subunit structure	Component of complex II composed of four subunits: a flavoprotein (FP), an iron-sulfur protein (IP), and a cytochrome b composed of a large and a small subunit By similarity.
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein; Matrix sideBy similarity.
Sequence similarities	Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family. Contains 1 2Fe-2S ferredoxin-type domain. Contains 1 4Fe-4S ferredoxin-type domain.

Ontologies

Keywords
Biological process	Electron transport Transport Tricarboxylic acid cycle
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Ligand	2Fe-2S 3Fe-4S 4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Transit peptide	1 – ?		Mitochondrion
	Chain	? – 283		Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial
Regions
	Domain	66 – 145	80	2Fe-2S ferredoxin-type
	Domain	186 – 216	31	4Fe-4S ferredoxin-type
Sites
	Metal binding	105	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	110	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	113	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	125	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	196	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	199	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	202	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	206	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	253	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	259	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	263	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Binding site	211	1	Ubiquinone; shared with DHSD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q70KF8-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 058945BABE06185F	FASTA	283	32,104
	10 20 30 40 50 60 MINIPNSIRP FIRSANRTPC YLRSISSSSS SSFATPAEEH AGKQPSSAVP VKEFSIYRWN 70 80 90 100 110 120 PDEPSKKPTL QTYSIDLKKC GPMVLDALIK IKNELDPTLT FRRSCREGIC GSCAMNIDGV 130 140 150 160 170 180 NTLACLKRIN KETSAPVKIY PLPHMYIIKD LVPDMTHFYK QYKSIEPFLK NDNPPAQGEF 190 200 210 220 230 240 LQSPEDRKKL DGMYECILCA CCSTSCPSYW WNQDEYLGPA VLMQAYRWMA DSRDSYGEDR 250 260 270 280 KEKLQNTLSL YRCHTIFNCT KTCPKGLNPA KAISHIKREM ASA

References

[1]

"Prelude to transformation: characterization of the beta-tubulin and succinate dehydrogenase genes from the rust fungus Uromyces fabae." Wirsel S.G.R., Voegele R.T., Baenninger R., Hahn M., Mendgen K.W. Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ575686 Genomic DNA. Translation: CAE02642.1.
3D structure databases
SMR	Q70KF8. Positions 51-282.
ModBase	Search...
Family and domain databases
InterPro	IPR006058. 2Fe2S_fd_BS. IPR001450. 4Fe4S_Fe_S_bd. IPR012675. b-grasp_ferredoxin-like. IPR001041. Ferredoxin. IPR012285. Fum_reductase_C. IPR004489. Succ_DHase/fum_Rdtase_Fe-S. [Graphical view]
Gene3D	G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit. G3DSA:1.10.1060.10. Fum_reductase_C. 1 hit.
TIGRFAMs	TIGR00384. dhsB. 1 hit.
PROSITE	PS00197. 2FE2S_FER_1. 1 hit. PS51085. 2FE2S_FER_2. 1 hit. PS00198. 4FE4S_FER_1. 1 hit. PS51379. 4FE4S_FER_2. 1 hit. [Graphical view]
ProDom	Q70KF8. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

DHSB_UROFA

Accession

Primary (citable) accession number: Q70KF8

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	July 5, 2004
Last modified:	September 2, 2008
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)