Reviewed, UniProtKB/Swiss-Prot Q766C3 (NEP1_NEPGR)

Names and origin

Protein names

Recommended name:
    Aspartic proteinase nepenthesin-1
    EC=3.4.23.12
Alternative name(s):
    Nepenthesin-I

Gene names

Name:

nep1

Organism

Nepenthes gracilis (Slender pitcher plant)

Taxonomic identifier

150966 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Nepenthaceae › Nepenthes

Protein attributes

Sequence length	437 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Extracellular proteinase found in the pitcher fluid of carnivorous plants. Digest prey for nitrogen uptake.
Catalytic activity	Similar to pepsin, but also cleaves on either side of Asp and at Lys-|-Arg.
Enzyme regulation	Inhibited by pepstatin and by diazoacetyl-D,L-norleucine methyl ester (DAN) in the presence of Cu(2+) ions By similarity.
Subcellular location	SecretedBy similarity.
Sequence similarities	Belongs to the peptidase A1 family.
Biophysicochemical properties	pH dependence: Optimum pH is 2.6. Retains 95% and 79% of the original activity after incubation for 30 days at pH 3.0 and pH 10.0 respectively. Temperature dependence: Optimum temperature is 55 degrees Celsius. Thermostable up to 50 degrees Celsius. Retains 60% of the original activity after incubation for 30 days at 50 degrees Celsius.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Domain	Signal
Molecular function	Aspartyl protease Hydrolase Protease
PTM	Glycoprotein Zymogen
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Propeptide	25 – 78	54	Activation peptide By similarity
	Chain	79 – 437	359	Aspartic proteinase nepenthesin-1
Sites
	Active site	113	1	By similarity
	Active site	315	1	By similarity
Amino acid modifications
	Glycosylation	53	1	N-linked (GlcNAc...) Potential
	Glycosylation	98	1	N-linked (GlcNAc...) Potential
	Glycosylation	131	1	N-linked (GlcNAc...) Potential
	Glycosylation	198	1	N-linked (GlcNAc...) Potential
	Glycosylation	267	1	N-linked (GlcNAc...) Potential
	Glycosylation	307	1	N-linked (GlcNAc...) Potential
	Glycosylation	345	1	N-linked (GlcNAc...) Potential
	Disulfide bond	123 ↔ 126		Probable
	Disulfide bond	129 ↔ 203		Probable
	Disulfide bond	150 ↔ 168		Probable
	Disulfide bond	155 ↔ 163		Probable
	Disulfide bond	240 ↔ 434		Probable
	Disulfide bond	354 ↔ 395		Probable
Natural variations
	Natural variant	233	1	D → V in nepenthesin-1b.
	Natural variant	251	1	N → T in nepenthesin-1b.
	Natural variant	392	1	G → E in nepenthesin-1b.

Sequences

	Sequence		Length	Mass (Da)
	Q766C3-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: A430C3A84ED3FF3E	FASTA	437	46,357
	10 20 30 40 50 60 MASSLYSFLL ALSIVYIFVA PTHSTSRTAL NHRHEAKVTG FQIMLEHVDS GKNLTKFQLL 70 80 90 100 110 120 ERAIERGSRR LQRLEAMLNG PSGVETSVYA GDGEYLMNLS IGTPAQPFSA IMDTGSDLIW 130 140 150 160 170 180 TQCQPCTQCF NQSTPIFNPQ GSSSFSTLPC SSQLCQALSS PTCSNNFCQY TYGYGDGSET 190 200 210 220 230 240 QGSMGTETLT FGSVSIPNIT FGCGENNQGF GQGNGAGLVG MGRGPLSLPS QLDVTKFSYC 250 260 270 280 290 300 MTPIGSSTPS NLLLGSLANS VTAGSPNTTL IQSSQIPTFY YITLNGLSVG STRLPIDPSA 310 320 330 340 350 360 FALNSNNGTG GIIIDSGTTL TYFVNNAYQS VRQEFISQIN LPVVNGSSSG FDLCFQTPSD 370 380 390 400 410 420 PSNLQIPTFV MHFDGGDLEL PSENYFISPS NGLICLAMGS SSQGMSIFGN IQQQNMLVVY 430 DTGNSVVSFA SAQCGAS

References

[1]

"Enzymic and structural characterization of nepenthesin, a unique member of a novel subfamily of aspartic proteinases." Athauda S.B.P., Matsumoto K., Rajapakshe S., Kuribayashi M., Kojima M., Kubomura-Yoshida N., Iwamatsu A., Shibata C., Inoue H., Takahashi K. Biochem. J. 381:295-306(2004) [PubMed: 15035659] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], 3D-STRUCTURE MODELING, CHARACTERIZATION, VARIANTS NEPENTHESIN IB VAL-233; THR-251 AND GLU-392. Tissue: Pitcher.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB114914 mRNA. Translation: BAD07474.1.
3D structure databases
HSSP	HSSP built from PDB template 1AVF based on UniProtKB P20142.
ModBase	Search...
Protein family/group databases
MEROPS	A01.040.
Family and domain databases
InterPro	IPR001969. Pept_Asp_AS. IPR009007. Pept_Aspartc_cat. IPR001461. Peptidase_A1. [Graphical view]
Gene3D	G3DSA:2.40.70.10. Pept_Aspartc_cat. 1 hit.
PANTHER	PTHR13683. Peptidase_A1. 1 hit.
Pfam	PF00026. Asp. 1 hit. [Graphical view]
PRINTS	PR00792. PEPSIN.
PROSITE	PS00141. ASP_PROTEASE. 1 hit. [Graphical view]
ProDom	Q766C3. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

NEP1_NEPGR

Accession

Primary (citable) accession number: Q766C3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	July 5, 2004
Last modified:	July 22, 2008
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)