Reviewed, UniProtKB/Swiss-Prot Q7TT50 (MRCKB_MOUSE)

Names and origin

Protein names

Recommended name:
    Serine/threonine-protein kinase MRCK beta
    EC=2.7.11.1
Alternative name(s):
    CDC42-binding protein kinase beta
    Myotonic dystrophy kinase-related CDC42-binding kinase beta
      Short name(s)=Myotonic dystrophy protein kinase-like beta, MRCK beta
    DMPK-like beta

Gene names

Name:	Cdc42bpb
Synonyms:	Kiaa1124

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	1713 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May act as a downstream effector of CDC42 in cytoskeletal reorganization. Contributes to the actomyosin contractility required for cell invasion, through the regulation of MYPT1 and thus MLC2 phosphorylation By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium By similarity.
Enzyme regulation	Maintained in an inactive, closed conformation by an interaction between the kinase domain and the negative autoregulatory C-terminal coiled-coil region. Agonist binding to the phorbol ester binding site disrupts this, releasing the kinase domain to allow N-terminus-mediated dimerization and kinase activation by transautophosphorylation By similarity.
Subunit structure	Homodimer and homotetramer via the coiled coil regions. Interacts tightly with GTP-bound but not GDP-bound CDC42 By similarity.
Subcellular location	CytoplasmBy similarity. Note= Displays a dispersed punctate distribution and concentrates along the cell periphery, especially at the leading edge and cell-cell junction. This concentration is PH-domain dependent By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 CNH domain. Contains 1 CRIB domain. Contains 1 PH domain. Contains 1 phorbol-ester/DAG-type zinc finger. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Coiled coil Phorbol-ester binding Zinc-finger
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	actin cytoskeleton reorganization Inferred from sequence or structural similarity. Source: UniProtKB protein amino acid phosphorylation Inferred from sequence or structural similarity. Source: UniProtKB regulation of small GTPase mediated signal transduction Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	intercellular junction Inferred from sequence or structural similarity. Source: UniProtKB leading edge Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	ATP binding Inferred from sequence or structural similarity. Source: UniProtKB identical protein binding Inferred from sequence or structural similarity. Source: UniProtKB magnesium ion binding Inferred from sequence or structural similarity. Source: UniProtKB protein serine/threonine kinase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 1713	1713	Serine/threonine-protein kinase MRCK beta
Regions
	Domain	76 – 342	267	Protein kinase
	Domain	343 – 413	71	AGC-kinase C-terminal
	Domain	1096 – 1215	120	PH
	Domain	1242 – 1515	274	CNH
	Domain	1585 – 1598	14	CRIB
	Nucleotide binding	82 – 90	9	ATP By similarity
	Zinc finger	1026 – 1076	51	Phorbol-ester/DAG-type
	Coiled coil	434 – 649	216	Potential
	Coiled coil	681 – 815	135	Potential
	Coiled coil	878 – 939	62	Potential
Sites
	Active site	200	1	Proton acceptor By similarity
	Binding site	105	1	ATP By similarity
Amino acid modifications
	Modified residue	221	1	Phosphoserine; by autocatalysis By similarity
	Modified residue	233	1	Phosphoserine; by autocatalysis By similarity
	Modified residue	239	1	Phosphothreonine; by autocatalysis By similarity
	Modified residue	914	1	Phosphoserine By similarity
	Modified residue	954	1	Phosphotyrosine
	Modified residue	1197	1	Phosphothreonine By similarity
	Modified residue	1692	1	Phosphoserine
	Modified residue	1695	1	Phosphoserine

Sequences

	Sequence		Length	Mass (Da)
	Q7TT50-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 0D63976EAC61DDE1	FASTA	1,713	194,781
	10 20 30 40 50 60 MSAKVRLKKL EQLLLDGPWR NDSALSVETL LDVLVCLYTE CSHSALRRDK YVAEFLEWAK 70 80 90 100 110 120 PFTQLVKDMQ LHREDFEIIK VIGRGAFGEV AVVKMRNTER IYAMKILNKW EMLKRAETAC 130 140 150 160 170 180 FREERDVLVN GDCQWITALH YAFQDENYLY LVMDYYVGGD LLTLLSKFED KLPEDMARFY 190 200 210 220 230 240 IGEMVLAIDS IHQLHYVHRD IKPDNVLLDV NGHIRLADFG SCLKMNDDGT VQSSVAVGTP 250 260 270 280 290 300 DYISPEILQA MEDGMGKYGP ECDWWSLGVC MYEMLYGETP FYAESLVETY GKIMNHEERF 310 320 330 340 350 360 QFPSHVTDVS EEAKDLIQRL ICSRERRLGQ NGIEDFKKHA FFEGLNWENI RNLEAPYIPD 370 380 390 400 410 420 VSSPSDTSNF DVDDDMLRNI EILPPGSHTG FSGLHLPFIG FTFTTESCFS DRGSLKSMTQ 430 440 450 460 470 480 SNTLTKDEDV QRDLENSLQI EAYERRIRRL EQEKLELSRK LQESTQTVQS LHGSTRALGN 490 500 510 520 530 540 SNRDKEIKRL NEELERMKSK MADSNRLERQ LEDTVTLRQE HEDSTHRLKG LEKQYRLARQ 550 560 570 580 590 600 EKEELHKQLV EASERLKSQT KELKDAHQQR KRALQEFSEL NERMSELRSL KQKVSRQLRD 610 620 630 640 650 660 KEEEMEVAMQ KIDSMRQDLR KSEKSRKELE ARLEDAAAEA SKERKLREHS ESFCKQMERE 670 680 690 700 710 720 LEALKVKQGG RGPGAASEHQ QEISKIRSEL EKKVLFYEEE LVRREASHVL EVKNVKKEVH 730 740 750 760 770 780 DSESHQLALQ KEVLMLKDKL EKSKRERHSE MEEAIGTVKD KYERERAMLF DENKKLTAEN 790 800 810 820 830 840 EKLCSFVDKL TAQNRQLEDE LQDLASKKES VAHWEAQIAE IIQWVSDEKD ARGYLQALAS 850 860 870 880 890 900 KMTEELETLR SSSLGSRTLD PLWKVRRSQK LDMSARLELQ SALEAEIRAK QLVQEELRKV 910 920 930 940 950 960 KDSSLAFESK LKESEAKNRE LLEEMQSLRK RMEEKFRADT GLKLPDFQDS IFEYFNTAPL 970 980 990 1000 1010 1020 AHDLTFRTSS ASDQETQASK MDLSPSVSVA TSTEQQEDMA RPQQRPSPVP LPSTQALAMA 1030 1040 1050 1060 1070 1080 GPKPKAHQFS IKSFPSPTQC SHCTSLMVGL IRQGYACEVC AFSCHVSCKD SAPQVCPIPP 1090 1100 1110 1120 1130 1140 EQSKRPLGVD VQRGIGTAYK GYVKVPKPTG VKKGWQRAYA VVCDCKLFLY DLPEGKSTQP 1150 1160 1170 1180 1190 1200 GVVASQVLDL RDEEFAVSSV LASDVIHATR RDIPCIFRVT ASLLGSPSKT SSLLILTENE 1210 1220 1230 1240 1250 1260 NEKRKWVGIL EGLQAILHKN RLKSQVVHVA QEAYDSSLPL IKAVLAAAIV DGDRIAVGLE 1270 1280 1290 1300 1310 1320 EGLYVIELTR DVIVRAADCK KVYQIELAPK EKIAILLCGR NHHVHLYPWS SFDGAEASNF 1330 1340 1350 1360 1370 1380 DIKLPETKGC QLIATGTLRK SSSTCLFVAV KRLILCYEIQ RTKPFHRKFS ELVAPGHVQW 1390 1400 1410 1420 1430 1440 MAVFKDRLCV GYPSGFSLLS IQGDGPPLDL VNPTDPSLAF LSQQSFDALC AVELKSEEYL 1450 1460 1470 1480 1490 1500 LCFSHMGLYV DPQGRRSRMQ ELMWPAAPVA CSCSPTHVTV YSEYGVDVFD VRTMEWVQTI 1510 1520 1530 1540 1550 1560 GLRRIRPLNS DGSLNLLGCE PPRLIYFKNK FSGTILNVPD TSDNSKKQML RTRSKRRFVF 1570 1580 1590 1600 1610 1620 KVPEEERLQQ RREMLRDPEL RSKMISNPTN FNHVAHMGPG DGMQVLMDLP LSAAPTVQEE 1630 1640 1650 1660 1670 1680 KQGPTPAGLP RQPPSRSKPY VSWPSSGGSE PGVPVPLRSM SDPDQDFDKE PDSDSTKHST 1690 1700 1710 PSNSSNPSGP PSPNSPHRSQ LPMEGLDQPS CDA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Huang C.Q., Wu S.L., Cheng Z. Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H. DNA Res. 11:205-218(2004) [PubMed: 15368895] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 628-1713. Tissue: Fetal brain.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1139-1713. Strain: FVB/N. Tissue: Kidney.
[4]	"Immunoaffinity profiling of tyrosine phosphorylation in cancer cells." Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J. Nat. Biotechnol. 23:94-101(2005) [PubMed: 15592455] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-954, MASS SPECTROMETRY.
[5]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed: 17242355] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1692 AND SER-1695, MASS SPECTROMETRY. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY277589 mRNA. Translation: AAP34402.1. AK173103 mRNA. Translation: BAD32381.1. BC049921 mRNA. Translation: AAH49921.1.
RefSeq	NP_898837.1.
UniGene	Mm.27397
3D structure databases
HSSP	HSSP built from PDB template 1MRY based on UniProtKB P31751.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q7TT50.
PTM databases
PhosphoSite	Q7TT50.
Genome annotation databases
Ensembl	ENSMUSG00000021279. Mus musculus. [Contig view]
GeneID	217866.
KEGG	mmu:217866.
Organism-specific databases
MGI	MGI:2136459. Cdc42bpb.
Rouge	Search...
Phylogenomic databases
HOGENOM	Q7TT50.
HOVERGEN	Q7TT50.
Gene expression databases
ArrayExpress	Q7TT50.
CleanEx	MM_CDC42BPB.
GermOnline	ENSMUSG00000021279. Mus musculus.
Family and domain databases
InterPro	IPR001180. Citron. IPR002219. DAG_PE_bd. IPR014930. DMPK_coil. IPR000095. PAK_box_Rho_bd. IPR001849. PH. IPR000961. Pkinase_C. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_bd_CS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00130. C1_1. 1 hit. PF00780. CNH. 1 hit. PF08826. DMPK_coil. 1 hit. PF00786. PBD. 1 hit. PF00169. PH. 1 hit. PF00069. Pkinase. 1 hit. PF00433. Pkinase_C. 1 hit. [Graphical view]
PRINTS	PR00008. DAGPEDOMAIN.
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00109. C1. 1 hit. SM00036. CNH. 1 hit. SM00285. PBD. 1 hit. SM00233. PH. 1 hit. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS50108. CRIB. 1 hit. PS50003. PH_DOMAIN. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. PS00479. ZF_DAG_PE_1. 1 hit. PS50081. ZF_DAG_PE_2. 1 hit. [Graphical view]
BLOCKS	Search...
Other Resources
SOURCE	Search...
ProtoNet	Search...

Entry information

Entry name

MRCKB_MOUSE

Accession

Primary (citable) accession number: Q7TT50
Secondary accession number(s): Q69ZR5, Q80W33

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
Last sequence update:	October 1, 2003
Last modified:	July 22, 2008
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)