Reviewed, UniProtKB/Swiss-Prot Q7VQG3 (SYA_BLOFL)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name(s)=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	Bfl168

Organism

Blochmannia floridanus [Complete proteome] [HAMAP]

Taxonomic identifier

203907 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › ant endosymbionts › Candidatus Blochmannia

Protein attributes

Sequence length	891 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 891	891	Alanyl-tRNA synthetase

Sequences

	Sequence		Length	Mass (Da)
	Q7VQG3-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 82DFE1E9BF88258B	FASTA	891	102,067
	10 20 30 40 50 60 MYKSIDVVRQ EFLDFFHKQE HQVVPSSSLV PKNDQTLLFT NSGMNQFKDI FLGLVKPSYK 70 80 90 100 110 120 RVVTAQRCMR AGGKHNDLNN VGYTECHLTF FEMLGNFSFG DYFKDDAIQF AWELLTDSNW 130 140 150 160 170 180 FGLSKDKIWV TTHIEDDESY NIWVKQVGIS TKRIVKIGNK NSDLYDSDNF WQMGNIGPCG 190 200 210 220 230 240 PCSEIFYDFG SDLKGKPPGY MQGLGSRYIE IWNLVFMQFN RQLNDRLIDL PMLSVDTGMG 250 260 270 280 290 300 LERITAILQG VHSNYLIDVF KNLIVDISNI MKVKEYINNR SLYVIADHIR ACVFLIKDGV 310 320 330 340 350 360 VPSNEGQGYV LRRIIRRSVR HGRKLGVNDI FLYKLVNLVI VHMNYVSNIL YDQKDLIEQI 370 380 390 400 410 420 LFNEEKLFNN TLKKGLELLE KSLKNLNQDK ILSGEIAFQL YTTYGFPLEL TKDICCERNI 430 440 450 460 470 480 KVDQLEFDQI MLTERRNSKR LSQFHKNFNN IILSSYAKTS TFVGYKCFSC QSKIIALLQD 490 500 510 520 530 540 NELINKMCDV DQESMVILDI TPFYGESGGQ IGDSGYLKGE CGVFKVKNTK KYGQIIVHIG 550 560 570 580 590 600 VLISGIFLIG EQVFAQVNRL KRKDISVNHS STHLLHSALL KILGSHVTQQ GSLINDKYFR 610 620 630 640 650 660 FDFSHYNAIT ITQINEVENL INQQIWDNLL VTENIMLMES ARNIGAIMLL HKQYTEKVRV 670 680 690 700 710 720 IQIGDCSIEL CGGTHVSNTN EIGLFIITKE FGIGSGVRRI EAVTKNAALS VIQNKKKLIQ 730 740 750 760 770 780 NIAQIAQSDD VNLLNVIHEF KARYEKLDRE IKLLNSKQEI QKVLSLVREV YYIKNVRVLV 790 800 810 820 830 840 NHVKNMESSS LFKMVKLLKH YLQSGIVVLI NIRKNNIAHI VISITKDLVE CNRIYAIDLI 850 860 870 880 890 QYIIRSVKNG KGGGRFDFAQ LNINNVKNVS ELVIQIQSLL NDIICNIKSI K

References

[1]

"The genome sequence of Blochmannia floridanus: comparative analysis of reduced genomes." Gil R., Silva F.J., Zientz E., Delmotte F., Gonzalez-Candelas F., Latorre A., Rausell C., Kamerbeek J., Gadau J., Hoelldobler B., van Ham R.C.H.J., Gross R., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:9388-9393(2003) [PubMed: 12886019] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX248583 Genomic DNA. Translation: CAD83689.1.
RefSeq	NP_878473.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1499368.
GenomeReviews	Gene locus Bfl168 in contig BX248583_GR.
KEGG	bfl:Bfl168.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7VQG3.
Enzyme and pathway databases
BioCyc	CBLO203907:BFL168-MON.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProDom	Q7VQG3. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

SYA_BLOFL

Accession

Primary (citable) accession number: Q7VQG3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	October 1, 2003
Last modified:	July 22, 2008
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)