Reviewed, UniProtKB/Swiss-Prot Q805F3 (CATEA_XENLA)

Names and origin

Protein names

Recommended name:
    Cathepsin E-A
    EC=3.4.23.34

Gene names

Name:	ctse-A
Synonyms:	ce1

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	397 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May have a role in immune function. Probably involved in the processing of antigenic peptides during MHC class II-mediated antigen presentation By similarity.
Catalytic activity	Similar to cathepsin D, but slightly broader specificity.
Subunit structure	Homodimer; disulfide-linked.
Subcellular location	EndosomeBy similarity. Note= The proenzyme is localized to the endoplasmic reticulum and Golgi apparatus, while the mature enzyme is localized to the endosome By similarity.
Tissue specificity	Expressed predominantly in the larval foregut and the anterior and posterior adult stomach.
Developmental stage	Expression levels are high in surface mucous cells and manicotto gland cells of the foregut epithelium of pro-metamorphic tadpoles. During metamorphosis, expression levels decrease markedly in larval epithelial cells but are high in proliferating adult epithelial primordia. In the adult stomach, expression was strongest in oxynticopeptic cells, but was also detected at a lower level in surface mucose cells.
Post-translational modification	Glycosylated. Contains high mannose-type oligosaccharide.
Sequence similarities	Belongs to the peptidase A1 family.

Ontologies

Keywords
Cellular component	Endosome
Domain	Signal
Molecular function	Aspartyl protease Hydrolase Protease
PTM	Glycoprotein Zymogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
Uncategorized	cathepsin E activity Ref.1 Inferred from direct assay. Source: UniProtKB
Biological process	antigen processing and presentation of exogenous peptide antigen via MHC class II Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	endosome Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Signal peptide	1 – 16	16
	Propeptide	17 – 52	36	Activation peptide
	Chain	53 – 397	345	Cathepsin E-A
Sites
	Active site	92	1	By similarity
	Active site	277	1	By similarity
Amino acid modifications
	Glycosylation	86	1	N-linked (GlcNAc...) Potential
	Glycosylation	130	1	N-linked (GlcNAc...) Potential
	Disulfide bond	105 ↔ 110		By similarity
	Disulfide bond	268 ↔ 272		By similarity
	Disulfide bond	310 ↔ 344		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q805F3-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 3DA99B6866E84611	FASTA	397	42,907
	10 20 30 40 50 60 MRQILVLLLF ATLVYGLIRV PLKRQKSIRK TLKEKGKLSH IWTQQGIDMV QYTDSCSNDQ 70 80 90 100 110 120 APSEPLINYM DVEYFGEISV GTPPQNFTVI FDTGSSNLWV PSVYCISQAC AQHDRFQPQL 130 140 150 160 170 180 SSTYESNGNN FSLQYGTGSL SGVIGIDAVT VEGILVQNQQ FGESVSEPGS TFVDAEFDGI 190 200 210 220 230 240 LGLGYPSIAV GDCTPVFDNM IAQNLVELPM FSVYMSRNPN SAVGGELVFG GFDASRFSGQ 250 260 270 280 290 300 LNWVPVTNQG YWQIQLDNVQ INGEVLFCSG GCQAIVDTGT SLITGPSSDI VQLQNIIGAS 310 320 330 340 350 360 AANGDYEVDC SVLNEMPTVT FTINGIGYQM TPQQYTLQDG GGVCSSGFQG LDIPPPAGPL 370 380 390 WILGDVFIGQ YYSVFDRGNN RVGLAPVVPY PPLKNGV

References

[1]

"Differential expression of two cathepsin Es during metamorphosis-associated remodeling of the larval to adult type epithelium in Xenopus stomach." Ikuzawa M., Yasumasu S., Inokuchi T., Kobayashi K., Nomura K., Iuchi I. J. Biochem. 134:385-394(2003) [PubMed: 14561724] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 17-25 AND 53-59, CATALYTIC ACTIVITY, SUBUNIT, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, GLYCOSYLATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB080684 mRNA. Translation: BAC57453.1.
RefSeq	NP_001079043.1.
UniGene	Xl.35410
3D structure databases
HSSP	HSSP built from PDB template 4CMS based on UniProtKB P00794.
SMR	Q805F3. Positions 64-388.
ModBase	Search...
Protein family/group databases
MEROPS	A01.010.
Genome annotation databases
GeneID	373572.
KEGG	xla:373572.
Phylogenomic databases
HOVERGEN	Q805F3.
Family and domain databases
InterPro	IPR001969. Pept_Asp_AS. IPR009007. Pept_Aspartc_cat. IPR001461. Peptidase_A1. IPR012848. Propep_A1. [Graphical view]
Gene3D	G3DSA:2.40.70.10. Pept_Aspartc_cat. 1 hit.
PANTHER	PTHR13683. Peptidase_A1. 1 hit.
Pfam	PF07966. A1_Propeptide. 1 hit. PF00026. Asp. 1 hit. [Graphical view]
PRINTS	PR00792. PEPSIN.
PROSITE	PS00141. ASP_PROTEASE. 2 hits. [Graphical view]
ProDom	Q805F3. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

CATEA_XENLA

Accession

Primary (citable) accession number: Q805F3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	June 1, 2003
Last modified:	July 22, 2008
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Xenopus annotation project