Reviewed, UniProtKB/Swiss-Prot Q81LK0 (SYA_BACAN)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	BA_4616, GBAA4616, BAS4284

Organism

Bacillus anthracis [Complete proteome] [HAMAP]

Taxonomic identifier

1392 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	880 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 880	880	Alanyl-tRNA synthetase

Sequences

	Sequence		Length	Mass (Da)
	Q81LK0-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 59EC95C8BB33BC3C	FASTA	880	97,441
	10 20 30 40 50 60 MKQLTGAQIR QMFLDFFQEK GHAVEPSASL VPHEDPSLLW INSGVATLKK YFDGRVIPQN 70 80 90 100 110 120 PRITNAQKSI RTNDIENVGK TARHHTFFEM LGNFSIGDYF KEEAITWAWE FLTSDKWIGF 130 140 150 160 170 180 DKELLSVTIH PEDEEAFTIW NEKMGVPKER IIRLEENFWD IGEGPSGPNT EIFYDRGEAY 190 200 210 220 230 240 GNDFSDPELY PGGENERYLE VWNLVFSQFN HNPDGSYTPL PKKNIDTGMG LERMTSIVQD 250 260 270 280 290 300 VPTNFDTDLF MPMIGATETI SGEKYRNGDL EKDMAFKVIA DHIRTVTFAV GDGALPSNEG 310 320 330 340 350 360 RGYVLRRLLR RAVRYSKKLN INRPFMFELV PVVGEVMKDF YPEVLEKKDF IAKVVKNEEE 370 380 390 400 410 420 RFHETLHDGE AILSEVIAKA KEEKTTVISG VDAFRLYDTY GFPIELTEEY AEEAGMTVDH 430 440 450 460 470 480 EGFENEMEKQ RERARAARQD VDSMQVQGGV LGEVKVASEF VGYGTVATES NVVALVKNGE 490 500 510 520 530 540 YTDSLQVGEE GQLMLDVTPF YAESGGQIAD RGCLLADGVK VLVKDVQKAP NGQNLHQVVV 550 560 570 580 590 600 EEGTLTKDAA VKAIIDTKNR SSVVKNHTAT HLLHQALKDV LGTHVNQAGS LVTSERLRFD 610 620 630 640 650 660 FSHFGQVQAD ELEKIERIVN EKIWESIDVE ISQKAIEEAK EMGAMALFGE KYGDVVRVVQ 670 680 690 700 710 720 VGDYSLELCG GCHVDNTASI GIFKIVAESG IGAGIRRIEA VTGKSAYELM NDQVGLLKEA 730 740 750 760 770 780 AGKMKTNPKD ILTRVDGLFA EVKQLQKENE SLAAKLSNIE AGNLTDSVMT VDGVNVLAAK 790 800 810 820 830 840 VNVADMNNLR TMMDDLKNKL ESAVVVLASV NDDKVNILAG VTKDLISQGY HAGKLVKEVA 850 860 870 880 SRCGGGGGGR PDMAQAGGKN PAQVEEALAF VQEYVKSVSK

References

[1]	"The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria." Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J. , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M. Nature 423:81-86(2003) [PubMed: 12721629] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames / isolate Porton.
[2]	"Bacillus anthracis comparative genomics." Ravel J., Rasko D.A., Shumway M.F., Jiang L., Cer R.Z., Federova N.B., Wilson M., Stanley S., Decker S., Read T.D., Salzberg S.L., Fraser C.M. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames ancestor.
[3]	"Complete genome sequence of Bacillus anthracis Sterne." Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sterne.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016879 Genomic DNA. Translation: AAP28321.1. AE017334 Genomic DNA. Translation: AAT33739.1. AE017225 Genomic DNA. Translation: AAT56583.1.
RefSeq	NP_846835.1. YP_021264.1. YP_030532.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1088737. 2815019. 2849985.
GenomeReviews	Gene locus BA_4616 in contig AE016879_GR. Gene locus BAS4284 in contig AE017225_GR. Gene locus GBAA4616 in contig AE017334_GR.
KEGG	ban:BA4616. bar:GBAA4616. bat:BAS4284.
TIGR	BA_4616. GBAA4616.
Phylogenomic databases
HOGENOM	Q81LK0.
Enzyme and pathway databases
BioCyc	BANT260799:BAS4284-MON. BANT261594:GBAA4616-MON.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProDom	Q81LK0. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

SYA_BACAN

Accession

Primary (citable) accession number: Q81LK0
Secondary accession number(s): Q6HT06, Q6KM96

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2003
Last sequence update:	June 1, 2003
Last modified:	July 22, 2008
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)