Reviewed, UniProtKB/Swiss-Prot Q83EL9 (RNPH_COXBU)

Names and origin

Protein names

Recommended name:
    Ribonuclease PH
      Short name=RNase PH
    EC=2.7.7.56
Alternative name(s):
    tRNA nucleotidyltransferase

Gene names

Name:	rph
Ordered Locus Names:	CBU_0299

Organism

Coxiella burnetii [Complete proteome] [HAMAP]

Taxonomic identifier

777 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella

Protein attributes

Sequence length	237 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates By similarity.
Catalytic activity	tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate.
Sequence similarities	Belongs to the RNase PH family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: HAMAP
Molecular function	tRNA binding Inferred from electronic annotation. Source: HAMAP tRNA nucleotidyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 237	237	Ribonuclease PH

Sequences

	Sequence		Length	Mass (Da)
	Q83EL9-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 445F30D58D5B4744	FASTA	237	25,971
	10 20 30 40 50 60 MRPSKRAANE LRPLSFTTQF TRYAEGSVLV TLGNTKVICN ASIVEGVPRF LKNSEQGWLT 70 80 90 100 110 120 AEYGMLPRST HSRMDREASR GKQGGRTVEI QRLIGRSLRA ALDLKLLGPY TITIDCDVIQ 130 140 150 160 170 180 ADGGTRTAAI NGSCIAMIEA LRHLQRKGIL QTDPLKHKVA AVSVGIYKGV PVLDLDYAED 190 200 210 220 230 SNAHTDMNVV MTDNDAFIEI QGTAEGDAFH AKELDALINL ARHGIKQIIE KQQEALS

References

[1]

"Complete genome sequence of the Q-fever pathogen, Coxiella burnetii." Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C., Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M., Lee K.H., Carty H.A. , Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E., Fraser C.M., Heidelberg J.F. Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003) [PubMed: 12704232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Nine Mile phase I / RSA 493.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016828 Genomic DNA. Translation: AAO89856.1.
RefSeq	NP_819342.1.
3D structure databases
HSSP	HSSP built from PDB template 1R6L based on UniProtKB P50597.
ModBase	Search...
Genome annotation databases
GeneID	1208181.
GenomeReviews	Gene locus CBU_0299 in contig AE016828_GR.
KEGG	cbu:CBU_0299.
NMPDR	fig|227377.1.peg.286.
TIGR	CBU_0299.
Phylogenomic databases
HOGENOM	Q83EL9.
Enzyme and pathway databases
BioCyc	CBUR227377:CBU_0299-MON.
Family and domain databases
HAMAP	MF_00564. [Tree]
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR002381. RNase_PH_bac-type. [Graphical view]
Pfam	PF01138. RNase_PH. 1 hit. PF03725. RNase_PH_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR01966. RNasePH. 1 hit.
PROSITE	PS01277. RIBONUCLEASE_PH. 1 hit. [Graphical view]
ProDom	Q83EL9. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

RNPH_COXBU

Accession

Primary (citable) accession number: Q83EL9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	June 1, 2003
Last modified:	July 22, 2008
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)