Reviewed, UniProtKB/Swiss-Prot Q83ET0 (RPOC_COXBU)

Names and origin

Protein names

Recommended name:
    DNA-directed RNA polymerase subunit beta'
      Short name=RNAP subunit beta'
    EC=2.7.7.6
Alternative name(s):
    Transcriptase subunit beta'
    RNA polymerase subunit beta'

Gene names

Name:	rpoC
Ordered Locus Names:	CBU_0232

Organism

Coxiella burnetii [Complete proteome] [HAMAP]

Taxonomic identifier

777 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella

Protein attributes

Sequence length	1414 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.
Sequence similarities	Belongs to the RNA polymerase beta' chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	transcription, DNA-dependent Inferred from electronic annotation. Source: HAMAP
Molecular function	DNA binding Inferred from electronic annotation. Source: HAMAP DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 1414	1414	DNA-directed RNA polymerase subunit beta'

Sequences

	Sequence		Length	Mass (Da)
	Q83ET0-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: A89BD7F533E3A5BE	FASTA	1,414	157,104
	10 20 30 40 50 60 MRDLVKQLKS EKHTAEFDAL RIKLASPEEV RSWSYGEVKK PETINYRTFK PEREGLFCAK 70 80 90 100 110 120 IFGPIKDYEC LCGKYKRLKH RGVICEKCGV EVTLAKVRRE RMGHIELASP VAHIWYLKSL 130 140 150 160 170 180 PSRIGLLLDV TLRDIERILY FEAYVVVDPG MTDLEPRQLL SEEAYLDALE EYGDDFTALM 190 200 210 220 230 240 GAEAIQRLLR DIDVEAEVEA LRTELQTTTS ETKTKKLTKR LKVLSAFLES GNKPEWMILT 250 260 270 280 290 300 VLPVLPPDLR PLVPLDGGRF ATSDLNDLYR RVINRNNRLK RLLDLNAPDI IVRNEKRMLQ 310 320 330 340 350 360 EAVDALLDNG RRGRAILGSN RRQLKSLADM IKGKSGRFRQ NLLGKRVDYS GRSVIVVGPT 370 380 390 400 410 420 LKLHQAGLPK KMALELFKPF IFSKLQLRGL ATTVKAAKKL VENEGPEVWD ILEEVIREHP 430 440 450 460 470 480 ILLNRAPTLH RLGIQAFEPV LVEGKAIQLH PLVCTAYNAD FDGDQMAVHV PLTLEAQLEA 490 500 510 520 530 540 RSLMMSTNNV LHPANGEPII VPSQDVVLGL YYITRDRVNA KGEGMRFADA QEVVRAYEND 550 560 570 580 590 600 QVDLHARITV RIKEGILNEA GEIEESDRLV NTAAGRILLW QIVPKGLPFA LVDQPMTKKA 610 620 630 640 650 660 VTKLLDFCYR NLGLKTTVIF ADKLMYMGFH YATHSGVSIG INDLVVPDQK EAIISRAEDE 670 680 690 700 710 720 VREIEKQYAS GLVTHGERRN KVIDIWSRTN DQVAKAMMEK IAVEKVKDAE GKEVAQSSFN 730 740 750 760 770 780 SIYMMSDSGA RGSAAQTRQL AGMRGLMARP DGTIIETPIT ANFREGLNVL QYFISTHGAR 790 800 810 820 830 840 KGLADTALKT ANSGYLTRRL VDVAQDLVVT EHDCGTEASI EMMPHIEGGD VVEPLRERVL 850 860 870 880 890 900 GRILAEPVMD PKSRKELLAK DTFLDERRVD ILEEHSIDRV RVRSAITCEA RYGICSMCYG 910 920 930 940 950 960 RDLARGHVVN VGEAIGVVAA QSIGEPGTQL TMRTFHIGGA ASRATAANNI GVKSTGKIKL 970 980 990 1000 1010 1020 RNLKTVEQAQ GNLVAVSRSG ELVVQDLQGS EREHYKVPYG ATISVRDGDS VKAGQIVAQW 1030 1040 1050 1060 1070 1080 DPHTHPIITE VAGTLRFVDL VDGVTMNRQT DELTGLSSIV ITSTKQRSAS GKELRPMVKL 1090 1100 1110 1120 1130 1140 VDKNDDDLFL PGGKVPAHYF LPEGTFLTKE DGTTVNIGDV LARIPQETSK TRDITGGLPR 1150 1160 1170 1180 1190 1200 VADLFEARRP KDAAILAEIS GVVSFGKDTK DKGRLIITAP DGTTHEELIP KWRHVSVFEG 1210 1220 1230 1240 1250 1260 ETVEKGEVIA DGPRDPHDIL RLLGVNALAN YIVNEVQEVY RLQGVKINDK HIEVIVRQML 1270 1280 1290 1300 1310 1320 RKVKITQPGD TDLLQNEQVE RTRVREENEK IIKKDGTVAK VEPILLGITK ASLATESFIS 1330 1340 1350 1360 1370 1380 AASFQETTRV LTAASVAGKR DDLRGLKENV IVGRLIPAGT GFSYHQQRRA VAGKSVEEKE 1390 1400 1410 IEEKRVTASE AEQALSEALK SSAPQEAKAA QKDE

References

[1]

"Complete genome sequence of the Q-fever pathogen, Coxiella burnetii." Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C., Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M., Lee K.H., Carty H.A. , Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E., Fraser C.M., Heidelberg J.F. Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003) [PubMed: 12704232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Nine Mile phase I / RSA 493.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016828 Genomic DNA. Translation: AAO89790.1.
RefSeq	NP_819276.1.
3D structure databases
HSSP	HSSP built from PDB template 1HQM based on UniProtKB Q9KWU6.
ModBase	Search...
Genome annotation databases
GeneID	1208113.
GenomeReviews	Gene locus CBU_0232 in contig AE016828_GR.
KEGG	cbu:CBU_0232.
NMPDR	fig|227377.1.peg.220.
TIGR	CBU_0232.
Phylogenomic databases
HOGENOM	Q83ET0.
Enzyme and pathway databases
BioCyc	CBUR227377:CBU_0232-MON.
Family and domain databases
HAMAP	MF_01322. [Tree]
InterPro	IPR000722. RNA_pol_A. IPR006592. RNA_pol_N. IPR007080. RNA_pol_Rpb1_1. IPR007066. RNA_pol_Rpb1_3. IPR007083. RNA_pol_Rpb1_4. IPR007081. RNA_pol_Rpb1_5. IPR012754. RpoC_beta_prime. [Graphical view]
Gene3D	G3DSA:2.40.40.30. RNA_pol_A. 1 hit. G3DSA:3.90.1120.10. RNA_pol_Rpb1_1. 1 hit.
Pfam	PF04997. RNA_pol_Rpb1_1. 1 hit. PF00623. RNA_pol_Rpb1_2. 1 hit. PF04983. RNA_pol_Rpb1_3. 1 hit. PF05000. RNA_pol_Rpb1_4. 1 hit. PF04998. RNA_pol_Rpb1_5. 1 hit. [Graphical view]
SMART	SM00663. RPOLA_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR02386. rpoC_TIGR. 1 hit.
ProDom	Q83ET0. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

RPOC_COXBU

Accession

Primary (citable) accession number: Q83ET0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	June 1, 2003
Last modified:	July 22, 2008
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)