Reviewed, UniProtKB/Swiss-Prot Q83EX7 (SYY_COXBU)

Names and origin

Protein names

Recommended name:
    Tyrosyl-tRNA synthetase
    EC=6.1.1.1
Alternative name(s):
    Tyrosine--tRNA ligase
      Short name(s)=TyrRS

Gene names

Name:	tyrS
Ordered Locus Names:	CBU_0181

Organism

Coxiella burnetii [Complete proteome] [HAMAP]

Taxonomic identifier

777 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella

Protein attributes

Sequence length	399 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) By similarity.
Catalytic activity	ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr).
Subunit structure	Homodimer By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 2 subfamily. Contains 1 S4 RNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tyrosyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP tyrosine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 399	399	Tyrosyl-tRNA synthetase
Regions
	Domain	337 – 398	62	S4 RNA-binding
	Motif	42 – 51	10	"HIGH" region
	Motif	226 – 230	5	"KMSKS" region
Sites
	Binding site	229	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q83EX7-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 1A622CD731D0316C	FASTA	399	45,830
	10 20 30 40 50 60 MISSQEAFEE IKRGAVEIIH EDELLSRLAE EKPLRIKLGF DPTAPDIHLG HTVVLNKLRQ 70 80 90 100 110 120 FQTLGHEVIF LIGDFTAMIG DPTGKNITRQ PLTREIVMEN TKTYETQAFR ILDPDKTQVT 130 140 150 160 170 180 SNSTWINKLK ADDLVRLAAT YTVARMLERD DFNKRYLSQQ PIAIHEFLYP LLQGYDSVAL 190 200 210 220 230 240 KADVELGGTD QKFNLLVGRE LQKHFGQRPQ CVLTVPLLEG LDGIQKMSKS LNNYIGITEP 250 260 270 280 290 300 PEEMFGKVMS ISDELMWRYY ELLSFRPMRE INRWREEVSE GRNPRDIKIL LAEELVTRFH 310 320 330 340 350 360 SKEAATKAHQ HFIDRFKRHE LPTDLDEIEL TAENTHLTIG YILQRAGLVN TTSEGLRMIA 370 380 390 QGAVRIDGER VEDIKLAIPR GESHLFQVGK RRFAKVKVI

References

[1]

"Complete genome sequence of the Q-fever pathogen, Coxiella burnetii." Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C., Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M., Lee K.H., Carty H.A. , Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E., Fraser C.M., Heidelberg J.F. Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003) [PubMed: 12704232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Nine Mile phase I / RSA 493.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016828 Genomic DNA. Translation: AAO89741.1.
RefSeq	NP_819227.1.
3D structure databases
HSSP	HSSP built from PDB template 1H3F based on UniProtKB P83453.
ModBase	Search...
Genome annotation databases
GeneID	1208053.
GenomeReviews	Gene locus CBU_0181 in contig AE016828_GR.
KEGG	cbu:CBU_0181.
NMPDR	fig|227377.1.peg.171.
TIGR	CBU_0181.
Phylogenomic databases
HOGENOM	Q83EX7.
Enzyme and pathway databases
BioCyc	CBUR227377:CBU_0181-MON.
Family and domain databases
HAMAP	MF_02007. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002305. aa-tRNA-synth_Ib. IPR014729. Rossmann-like_a/b/a_fold. IPR002942. S4_RNA_bd. IPR002307. Tyr-tRNA-synth_Ib_bac/mito. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11766. Tyr_tRNA-synt_1b. 1 hit.
Pfam	PF01479. S4. 1 hit. PF00579. tRNA-synt_1b. 1 hit. [Graphical view]
PRINTS	PR01040. TRNASYNTHTYR.
SMART	SM00363. S4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00234. tyrS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50889. S4. 1 hit. [Graphical view]
ProDom	Q83EX7. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

SYY_COXBU

Accession

Primary (citable) accession number: Q83EX7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	June 1, 2003
Last modified:	July 22, 2008
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)