Reviewed, UniProtKB/Swiss-Prot Q83F02 (QUEF_COXBU)

Names and origin

Protein names

Recommended name:
    NADPH-dependent 7-cyano-7-deazaguanine reductase
    EC=1.7.1.13
Alternative name(s):
    7-cyano-7-carbaguanine reductase
    PreQ(0) reductase
    NADPH-dependent nitrile oxidoreductase

Gene names

Name:	queF
Ordered Locus Names:	CBU_0151

Organism

Coxiella burnetii [Complete proteome] [HAMAP]

Taxonomic identifier

777 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella

Protein attributes

Sequence length	278 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity.
Catalytic activity	7-aminomethyl-7-carbaguanine + 2 NADP(+) = 7-cyano-7-carbaguanine + 2 NADPH.
Pathway	tRNA modification; queuosine-tRNA biosynthesis.
Subcellular location	CytoplasmProbable.
Sequence similarities	Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.

Ontologies

Keywords
Biological process	Queuosine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	queuosine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 278	278	NADPH-dependent 7-cyano-7-deazaguanine reductase

Sequences

	Sequence		Length	Mass (Da)
	Q83F02-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 10530DB1A19EF396	FASTA	278	32,018
	10 20 30 40 50 60 MSTLRVLHEK SELGKTTVYP KEYAPHLLLP IPRDLNRKTL NVNVSEPPPF YGYDLWNAYE 70 80 90 100 110 120 LSWLNEKGKP FAARGEFIIP ATSSHLIESK SFKLYLNSFN NERFADAAAV SQTMKRDLSK 130 140 150 160 170 180 RVNESVTVNF ILHETEIPVA YSPKGSLLDV LDIAIDTYSP DPNLLSTSQE TVTETLYSHL 190 200 210 220 230 240 LKSNCPVTGQ PDWGSIEIHY TGPKIDHVQL LKYIISYRNH EEFHEACVER FFMDILRHCR 250 260 270 PQELTVQARY TRRGGLDINP YRSTNPTFSV QNHRSFRQ

References

[1]

"Complete genome sequence of the Q-fever pathogen, Coxiella burnetii." Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C., Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M., Lee K.H., Carty H.A. , Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E., Fraser C.M., Heidelberg J.F. Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003) [PubMed: 12704232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Nine Mile phase I / RSA 493.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016828 Genomic DNA. Translation: AAO89715.1.
RefSeq	NP_819201.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1208022.
GenomeReviews	Gene locus CBU_0151 in contig AE016828_GR.
KEGG	cbu:CBU_0151.
NMPDR	fig|227377.1.peg.145.
TIGR	CBU_0151.
Phylogenomic databases
HOGENOM	Q83F02.
Enzyme and pathway databases
BioCyc	CBUR227377:CBU_0151-MON.
Family and domain databases
HAMAP	MF_00817. [Tree]
InterPro	IPR016428. CN_OxRdtase_NADPH-dep_YqcD. IPR001474. GTP_CycOHase_I. [Graphical view]
Pfam	PF01227. GTP_cyclohydroI. 1 hit. [Graphical view]
PIRSF	PIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMs	TIGR03138. QueF. 1 hit.
ProDom	Q83F02. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

QUEF_COXBU

Accession

Primary (citable) accession number: Q83F02

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	June 1, 2003
Last modified:	September 2, 2008
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)