Reviewed, UniProtKB/Swiss-Prot Q83F17 (MURC_COXBU)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramate--L-alanine ligase
    EC=6.3.2.8
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanine synthetase

Gene names

Name:	murC
Ordered Locus Names:	CBU_0136

Organism

Coxiella burnetii [Complete proteome] [HAMAP]

Taxonomic identifier

777 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella

Protein attributes

Sequence length	465 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine.
Pathway	Cell wall biosynthesis; peptidoglycan biosynthesis.
Subcellular location	CytoplasmProbable.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramate-L-alanine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 465	465	UDP-N-acetylmuramate--L-alanine ligase
Regions
	Nucleotide binding	115 – 121	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q83F17-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 5B2953C14A59991B	FASTA	465	50,640
	10 20 30 40 50 60 MQLDKKIINH VHCLGIGGIG VSALAEILLK KGCRVTGSDV SPNKNTERLQ RLGAEIIFNH 70 80 90 100 110 120 DGTAITQADC AVYSSAIGAT NPELMAAKQA KIPLLKRGEM LANLMKEYQS IAVAGAHGKT 130 140 150 160 170 180 TTSGMLSHAF VEANLDPTFM VGGVLNNSQT PARVGNGHYF IAEADESDAS FLFMHPDIAV 190 200 210 220 230 240 VTNIDADHLS TYDGDFNRLK QTYIQFLEQT AQDGVVVLCL DDPILREIAP LLSRRVITYG 250 260 270 280 290 300 FSSDAQYRVV DYCQQGIQSL FQIHSPQRKA PLTVKLSMPG QHNALNATAV TAIADVVQMN 310 320 330 340 350 360 EPALLKSLAD FPGVDRRFTI RGEMILPKGN ALIIEDYGHH PNEIKATLAA ARAAWPERRM 370 380 390 400 410 420 VLVFQPHRYS RTRDLMTEFV SVLAETDWLV LLEVYSAGEM PIPGADGMAL IKMMSNGMAQ 430 440 450 460 KTTFVPLLQN LPETLQKLSQ PNDIIILQGA GNIGSIVTAL VQTHG

References

[1]

"Complete genome sequence of the Q-fever pathogen, Coxiella burnetii." Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C., Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M., Lee K.H., Carty H.A. , Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E., Fraser C.M., Heidelberg J.F. Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003) [PubMed: 12704232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Nine Mile phase I / RSA 493.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016828 Genomic DNA. Translation: AAO89700.1.
RefSeq	NP_819186.1.
3D structure databases
HSSP	HSSP built from PDB template 1GQY based on UniProtKB P45066.
ModBase	Search...
Genome annotation databases
GeneID	1208007.
GenomeReviews	Gene locus CBU_0136 in contig AE016828_GR.
KEGG	cbu:CBU_0136.
NMPDR	fig|227377.1.peg.130.
TIGR	CBU_0136.
Phylogenomic databases
HOGENOM	Q83F17.
Enzyme and pathway databases
BioCyc	CBUR227377:CBU_0136-MON.
Family and domain databases
HAMAP	MF_00046. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR016040. NAD(P)-bd. IPR005758. UDP-N-AcMur_Ala_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01082. murC. 1 hit.
ProDom	Q83F17. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

MURC_COXBU

Accession

Primary (citable) accession number: Q83F17

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	June 1, 2003
Last modified:	July 22, 2008
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)