Reviewed, UniProtKB/Swiss-Prot Q83F55 (CLPB_COXBU)

Names and origin

Protein names

Recommended name:
    Chaperone protein clpB

Gene names

Name:	clpB
Ordered Locus Names:	CBU_0094

Organism

Coxiella burnetii [Complete proteome] [HAMAP]

Taxonomic identifier

777 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella

Protein attributes

Sequence length	859 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with dnaK, dnaJ and grpE. Acts before dnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase activity; ATP hydrolysis unfolds the denatured protein aggregates, which probably helps expose new hydrophobic binding sites on the surface of clpB-bound aggregates, contributing to the solubilization and refolding of denatured protein aggregates by dnaK By similarity.
Subunit structure	Homohexamer. The oligomerization is ATP-dependent By similarity.
Subcellular location	CytoplasmProbable.
Domain	The N-terminal domain probably functions as a substrate-discriminating domain, recruiting aggregated proteins to the clpB hexamer and/or stabilizing bound proteins. The NBD2 domain is responsible for oligomerization, whereas the NBD1 domain stabilizes the hexamer probably in an ATP-dependent manner. The movement of the coiled-coil domain is essential for clpB ability to rescue proteins from an aggregated state, probably by pulling apart large aggregated proteins, which are bound between the coiled-coils motifs of adjacent clpB subunits in the functional hexamer By similarity.
Sequence similarities	Belongs to the clpA/clpB family.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Cytoplasm
Domain	Coiled coil Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Chaperone
Technical term	Complete proteome
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 859	859	Chaperone protein clpB
Regions
	Nucleotide binding	206 – 213	8	ATP 1 By similarity
	Nucleotide binding	607 – 614	8	ATP 2 By similarity
	Region	1 – 143	143	N-terminal By similarity
	Region	159 – 340	182	NBD1 By similarity
	Region	341 – 547	207	Linker By similarity
	Region	557 – 766	210	NBD2 By similarity
	Region	767 – 859	93	C-terminal By similarity
	Coiled coil	391 – 525	135	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q83F55-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: D0D834A1F973197D	FASTA	859	96,769
	10 20 30 40 50 60 MRIDKFTTAF QTALADAQSL AVGRDHQFIE PAHVMKVLLE QTQGTVAPLL EQSKVNLSRL 70 80 90 100 110 120 IDGVNKAIDS YPQVEGTGGE VHVSRELSKI LTLMDKFAQQ NKDQYISSEW FIPAALEAKG 130 140 150 160 170 180 QLRDVLIEAG ADKKAIEKNI MNLRKGERVT EQSAEDQRQA LAKYTIDLTE KAETGKLDPV 190 200 210 220 230 240 IGRDEEIRRT VQVLQRRTKN NPVLIGEPGV GKTAIVEGLA QRIVNGEVPE GLKQKRLLAL 250 260 270 280 290 300 DMGALIAGAK FRGEFEERLK AVLKDIAKEE GRVILFIDEL HTMVGAGKAE GAMDAGNMLK 310 320 330 340 350 360 PALARGELHC VGATTLDEYR KYIEKDAALE RRFQKVLVEE PSTEDAIAIL RGLKERYEVH 370 380 390 400 410 420 HGVEITDPAI IAAATLSQRY ITDRNLPDKA IDLIDEAASQ IRMEMDSKPV ELDRLERRLI 430 440 450 460 470 480 QLKIEREALK KETDEASKKR LSDLETEIKN VEKEYSDLEE VWKSEKASLH GTQQIKEELE 490 500 510 520 530 540 QARIELEAAG RAGDLARMSE LQYGIIPELD KKLKAASQKE EQFHDHKLLR SRVTEEEVAE 550 560 570 580 590 600 VVSKWTHIPV SKMLEGEREK LLHMETELHK RVIGQDEAVN AVANAIRRSR AGLSDPNRPV 610 620 630 640 650 660 GSFLFLGPTG VGKTELCKAL AVFLFDTEDA MVRIDMSEFM EKHSVARLIG APPGYVGYEE 670 680 690 700 710 720 GGYLTEAIRR RPYSVILLDE IEKAHNDVFN VLLQVLDDGR LTDGQGRTVD FRNTVIVMTS 730 740 750 760 770 780 NLGSDLIREF SGENYDKMKD AVMEVVAQHF RPEFINRIDE AVVFHSLKKE QIRNIAIIQI 790 800 810 820 830 840 DRIKKRLKEK DYQLTISDDA LDYLSELGYD PVYGARPLKR VLQQQLENPL SQKILEGKFV 850 PGSLINIEKK GEQLEFKEA

References

[1]

"Complete genome sequence of the Q-fever pathogen, Coxiella burnetii." Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C., Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M., Lee K.H., Carty H.A. , Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E., Fraser C.M., Heidelberg J.F. Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003) [PubMed: 12704232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Nine Mile phase I / RSA 493.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016828 Genomic DNA. Translation: AAO89660.1.
RefSeq	NP_819146.1.
3D structure databases
HSSP	HSSP built from PDB template 1JBK based on UniProtKB P03815.
ModBase	Search...
Genome annotation databases
GeneID	1207964.
GenomeReviews	Gene locus CBU_0094 in contig AE016828_GR.
KEGG	cbu:CBU_0094.
NMPDR	fig|227377.1.peg.90.
TIGR	CBU_0094.
Phylogenomic databases
HOGENOM	Q83F55.
Enzyme and pathway databases
BioCyc	CBUR227377:CBU_0094-MON.
Family and domain databases
InterPro	IPR003593. AAA+_ATPase_core. IPR003959. AAA_ATPase_core. IPR013093. ATPase_AAA-2. IPR017730. Chaperonin_ClpB. IPR001270. Chaprnin_clpA/B. IPR004176. Clp_N. [Graphical view]
Pfam	PF00004. AAA. 1 hit. PF07724. AAA_2. 1 hit. PF02861. Clp_N. 2 hits. [Graphical view]
PRINTS	PR00300. CLPPROTEASEA.
SMART	SM00382. AAA. 2 hits. [Graphical view]
PROSITE	PS00870. CLPAB_1. 1 hit. PS00871. CLPAB_2. 1 hit. [Graphical view]
ProDom	Q83F55. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

CLPB_COXBU

Accession

Primary (citable) accession number: Q83F55

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	June 1, 2003
Last modified:	July 22, 2008
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)