Reviewed, UniProtKB/Swiss-Prot Q7YQE1 (GCNT3_BOVIN)

Names and origin

Protein names

Recommended name:
    Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3
    EC=2.4.1.102
    EC=2.4.1.150
Alternative name(s):
    C2GnT-mucin type
    bC2GnT-M
      Short name=C2GnT-M
    Mucus-type core 2 beta-1,6-N-acetylglucosaminyltransferase

Gene names

Name:

GCNT3

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	440 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans, leading to introduce the blood group I antigen during embryonic development By similarity.
Catalytic activity	UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl-R = UDP + beta-D-galactosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R. UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-1,6-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane proteinBy similarity.
Tissue specificity	Primarily expressed in mucus-secreting tissues.
Post-translational modification	N-glycosylated Probable.
Sequence similarities	Belongs to the glycosyltransferase 14 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: EC beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 440	440	Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3		PRO_0000288543
Regions
	Topological domain	1 – 12	12	Cytoplasmic Potential
	Transmembrane	13 – 30	18	Signal-anchor for type II membrane Potential
	Topological domain	31 – 440	410	Lumenal Potential
Amino acid modifications
	Glycosylation	72	1	N-linked (GlcNAc...) Potential
	Glycosylation	108	1	N-linked (GlcNAc...) Potential
	Disulfide bond	73 ↔ 230
	Disulfide bond	164 ↔ 384
	Disulfide bond	185 ↔ 212
	Disulfide bond	393 ↔ 425
Experimental info
	Sequence conflict	72	1	N → S in AAO22165. Ref.1
	Sequence conflict	263	1	Y → S in AAP76324 and AAP76326. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q7YQE1-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 94D0EF6A08D9DA10	FASTA	440	50,953
	10 20 30 40 50 60 MKMTGWKKKL CRGHHLWALG CYMLLAVVAL RLSLRLKCDV DSLDLESRDF QSQRCRDVLY 70 80 90 100 110 120 KNLKLPAKRS INCSGITRGD QEAVVQALLD NLEVKKKRLP FTDTYYLNIT RDCEQFKAQR 130 140 150 160 170 180 KFIQFPLSKE ELDFPIAYSM VVHEKIENFE RLLRAVYAPQ NIYCVHVDVK SPETFKEAVK 190 200 210 220 230 240 AIISCFPNVF MASKLVPVVY ASWSRVQADL NCMEDLLQSS VPWKYLLNTC GTDFPIKTNA 250 260 270 280 290 300 EMVLALKMLN GKNSMESEIP SEYKKNRWKY RYEVTDRLYL TSKMKDPPPD NLPMFTGNAY 310 320 330 340 350 360 FVASRAFVQH VLENPKSQRL IEWVKDTYSP DEHLWATLQR APWMPGSVPY HPKYHISDMT 370 380 390 400 410 420 AIARLVKWQG HEGDVSMGAP YAPCSGIHQR AICIYGAGDL HWILQNHHLL ANKFDPRVDD 430 440 NVLQCLEEYL RHKAIYGTEL

References

[1]	"The core 2 beta-1,6-N-acetylglucosaminyltransferase-mucin encoded by bovine herpesvirus 4 was acquired from an ancestor of the African buffalo." Markine-Goriaynoff N., Georgin J.-P., Goltz M., Zimmermann W., Broll H., Wamwayi H.M., Pastoret P.-P., Sharp P.M., Vanderplasschen A. J. Virol. 77:1784-1792(2003) [PubMed: 12525612] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Mucin biosynthesis: bovine C2GnT-M gene, tissue-specific expression, and herpes virus-4 homologue." Choi K.H., Osorio F.A., Cheng P.-W. Am. J. Respir. Cell Mol. Biol. 30:710-719(2004) [PubMed: 14592928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Tissue: Colon and Tracheal epithelium.
[3]	"Identification of disulfide bonds among the nine core 2 N-acetylglucosaminyltransferase-M cysteines conserved in the mucin beta6-N-acetylglucosaminyltransferase family." Singh J., Khan G.A., Kinarsky L., Cheng H., Wilken J., Choi K.H., Bedows E., Sherman S., Cheng P.-W. J. Biol. Chem. 279:38969-38977(2004) [PubMed: 15226299] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE, DISULFIDE BONDS, GLYCOSYLATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF465338 Genomic DNA. Translation: AAO22165.1. AY283763 mRNA. Translation: AAP76324.1. AY283764 mRNA. Translation: AAP76325.1. AY283765 mRNA. Translation: AAP76326.1. AY283766 mRNA. Translation: AAP76327.1. AY283767 Genomic DNA. Translation: AAP76328.1.
RefSeq	NP_991378.1.
UniGene	Bt.51854
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000009443. Bos taurus. [Contig view]
GeneID	404167.
KEGG	bta:404167.
Phylogenomic databases
HOVERGEN	Q7YQE1.
Family and domain databases
InterPro	IPR003406. Glyco_trans_14. [Graphical view]
PANTHER	PTHR19297. Glyco_trans_14. 1 hit.
Pfam	PF02485. Branch. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GCNT3_BOVIN

Accession

Primary (citable) accession number: Q7YQE1
Secondary accession number(s): Q7YQF8, Q866Z3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	October 1, 2003
Last modified:	November 4, 2008
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)