Reviewed, UniProtKB/Swiss-Prot Q86UL3 (PLCF_HUMAN)

Names and origin

Protein names

Recommended name:
    1-acyl-sn-glycerol-3-phosphate acyltransferase zeta
    EC=2.3.1.51
Alternative name(s):
    1-AGP acyltransferase 6
      Short name(s)=1-AGPAT 6
    Lysophosphatidic acid acyltransferase zeta
      Short name(s)=LPAAT-zeta

Gene names

Name:	AGPAT6
ORF Names:	UNQ551/PRO1108

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	456 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone By similarity.
Catalytic activity	Acyl-CoA + 1-acyl-sn-glycerol 3-phosphate = CoA + 1,2-diacyl-sn-glycerol 3-phosphate.
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 2/3.
Subcellular location	Membrane; Multi-pass membrane proteinPotential.
Tissue specificity	Relatively high level of expression in skeletal muscle, heart and testis. Relatively low level of expression in lung.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity.
Sequence similarities	Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Signal Transmembrane
Molecular function	Acyltransferase Transferase
PTM	Glycoprotein Phosphoprotein
Gene Ontology (GO)
Biological process	lactation Inferred from sequence or structural similarity. Source: UniProtKB triacylglycerol biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	endoplasmic reticulum Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Signal peptide	1 – 37	37	Potential
	Chain	38 – 456	419	1-acyl-sn-glycerol-3-phosphate acyltransferase zeta
Regions
	Transmembrane	156 – 176	21	Potential
	Transmembrane	180 – 200	21	Potential
	Motif	248 – 253	6	HXXXXD motif
Amino acid modifications
	Modified residue	218	1	Phosphotyrosine
	Glycosylation	247	1	N-linked (GlcNAc...) Potential
	Glycosylation	327	1	N-linked (GlcNAc...) Potential
	Glycosylation	328	1	N-linked (GlcNAc...) Potential
	Glycosylation	362	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q86UL3-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 5B9DEB2912E989E5	FASTA	456	52,071
	10 20 30 40 50 60 MFLLLPFDSL IVNLLGISLT VLFTLLLVFI IVPAIFGVSF GIRKLYMKSL LKIFAWATLR 70 80 90 100 110 120 MERGAKEKNH QLYKPYTNGI IAKDPTSLEE EIKEIRRSGS SKALDNTPEF ELSDIFYFCR 130 140 150 160 170 180 KGMETIMDDE VTKRFSAEEL ESWNLLSRTN YNFQYISLRL TVLWGLGVLI RYCFLLPLRI 190 200 210 220 230 240 ALAFTGISLL VVGTTVVGYL PNGRFKEFMS KHVHLMCYRI CVRALTAIIT YHDRENRPRN 250 260 270 280 290 300 GGICVANHTS PIDVIILASD GYYAMVGQVH GGLMGVIQRA MVKACPHVWF ERSEVKDRHL 310 320 330 340 350 360 VAKRLTEHVQ DKSKLPILIF PEGTCINNTS VMMFKKGSFE IGATVYPVAI KYDPQFGDAF 370 380 390 400 410 420 WNSSKYGMVT YLLRMMTSWA IVCSVWYLPP MTREADEDAV QFANRVKSAI ARQGGLVDLL 430 440 450 WDGGLKREKV KDTFKEEQQK LYSKMIVGNH KDRSRS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and identification of the human LPAAT-zeta gene, a novel member of the lysophosphatidic acid acyltransferase family." Li D., Yu L., Wu H., Shan Y., Guo J., Dang Y., Wei Y., Zhao S. J. Hum. Genet. 48:438-442(2003) [PubMed: 12938015] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[2]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed: 12975309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Ovary.
[4]	"Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra." Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D. J. Proteome Res. 6:4150-4162(2007) [PubMed: 17924679] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-218, MASS SPECTROMETRY. Tissue: Epithelium.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF406612 mRNA. Translation: AAP21893.1. AY358670 mRNA. Translation: AAQ89033.1. BC051377 mRNA. Translation: AAH51377.2. BC061884 mRNA. Translation: AAH61884.1.
RefSeq	NP_848934.1.
UniGene	Hs.355753
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSG00000158669. Homo sapiens. [Contig view]
GeneID	137964.
KEGG	hsa:137964.
Organism-specific databases
H-InvDB	HIX0004346.
HGNC	HGNC:20880. AGPAT6.
HPA	HPA016471.
MIM	608143. gene.
PharmGKB	PA142672637.
GenAtlas	Search...
GeneCards	Search...
GeneLynx	Search...
Phylogenomic databases
HOGENOM	Q86UL3.
HOVERGEN	Q86UL3.
Gene expression databases
ArrayExpress	Q86UL3.
CleanEx	HS_AGPAT6.
GermOnline	ENSG00000158669. Homo sapiens.
Family and domain databases
InterPro	IPR002123. Acyltransferase. [Graphical view]
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
SMART	SM00563. PlsC. 1 hit. [Graphical view]
ProDom	Q86UL3. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
LinkHub	Q86UL3.
SOURCE	Search...
ProtoNet	Search...

Entry information

Entry name

PLCF_HUMAN

Accession

Primary (citable) accession number: Q86UL3
Secondary accession number(s): Q86V89

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	June 1, 2003
Last modified:	July 22, 2008
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)