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Reviewed, UniProtKB/Swiss-Prot O60502 (NCOAT_HUMAN)

Last modified November 25, 2008. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Bifunctional protein NCOAT
Alternative name(s):
    Nuclear cytoplasmic O-GlcNAcase and acetyltransferase
    Meningioma-expressed antigen 5
Including the following 2 domains:
    1- Recommended name:
            Beta-hexosaminidase
              EC=3.2.1.52
        Alternative name(s):
            N-acetyl-beta-glucosaminidase
            Beta-N-acetylhexosaminidase
            Hexosaminidase C
            N-acetyl-beta-D-glucosaminidase
            O-GlcNAcase
    2- Recommended name:
            Histone acetyltransferase
                Short name=HAT
              EC=2.3.1.48
Gene names
Name: MGEA5
Synonyms: HEXC, KIAA0679, MEA5
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length916 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Cleaves GlcNAc but not GalNAc from glycopeptides. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc. Possesses hyaluronidase activity. Acetylates 'Lys-8' of histone H4 and 'Lys-14' of histone H3 By similarity.

Catalytic activity

Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.

Acetyl-CoA + histone = CoA + acetylhistone.

Enzyme regulation

Inhibited by N-acetylglucosamine and not N-acetylgalactosamine.

Subunit structure

Monomer. Binds both acetylated and unacetylated histone H4 tail but acetylation on 'Lys-8' of histone H4 abolishes binding By similarity.

Subcellular location

Isoform 3: Nucleus.

Isoform 1: Cytoplasm.

Tissue specificity

Ubiquitous. Shows highest expression in the brain, placenta and pancreas.

Post-translational modification

Proteolytically cleaved by caspase-3.

Biophysicochemical properties

Kinetic parameters:

KM=1.1 mM for pNP-GLcNAc

Vmax=652 µmol/min/mg enzyme with pNP-GLcNAc as substrate

pH dependence:

Optimum pH is 5.7-7.

Sequence caution

The sequence AAH47877.1 differs from that shown. Reason: Miscellaneous discrepancy. Contaminating sequence. Potential poly-A sequence.

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Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O60502-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O60502-2)

The sequence of this isoform differs from the canonical sequence as follows:
     346-398: Missing.
     691-704: Missing.
Isoform 3 (identifier: O60502-3)

Also known as: MGEA5s;

The sequence of this isoform differs from the canonical sequence as follows:
     663-677: CRSHSSAQFLIGDQE → RCTRNNLFSSNILSL
     678-916: Missing.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 916916Bifunctional protein NCOAT
PRO_0000252118

Regions

Region583 – 916334Histone acetyltransferase activity By similarity
Region695 – 814120Required for histone H4 binding By similarity

Sites

Active site1751Nucleophile; for O-GlcNAcase activity By similarity
Active site1771Proton donor; for O-GlcNAcase activity By similarity

Amino acid modifications

Modified residue3641Phosphoserine
Disulfide bond777 ↔ 793 By similarity

Natural variations

Alternative sequence346 – 39853Missing in isoform 2.
VSP_020866
Alternative sequence663 – 67715CRSHS…IGDQE → RCTRNNLFSSNILSL in isoform 3.
VSP_020867
Alternative sequence678 – 916239Missing in isoform 3.
VSP_020868
Alternative sequence691 – 70414Missing in isoform 2.
VSP_020869
Natural variant461G → E: dbSNP rs3740421.
VAR_027761
Natural variant6021E → K: dbSNP rs17853930.
VAR_027762

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Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2001. Version 2.
Checksum: 01F8A64A9B1475C6

FASTA916102,915
        10         20         30         40         50         60 
MVQKESQATL EERESELSSN PAASAGASLE PPAAPAPGED NPAGAGGAAV AGAAGGARRF 

        70         80         90        100        110        120 
LCGVVEGFYG RPWVMEQRKE LFRRLQKWEL NTYLYAPKDD YKHRMFWREM YSVEEAEQLM 

       130        140        150        160        170        180 
TLISAAREYE IEFIYAISPG LDITFSNPKE VSTLKRKLDQ VSQFGCRSFA LLFDDIDHNM 

       190        200        210        220        230        240 
CAADKEVFSS FAHAQVSITN EIYQYLGEPE TFLFCPTEYC GTFCYPNVSQ SPYLRTVGEK 

       250        260        270        280        290        300 
LLPGIEVLWT GPKVVSKEIP VESIEEVSKI IKRAPVIWDN IHANDYDQKR LFLGPYKGRS 

       310        320        330        340        350        360 
TELIPRLKGV LTNPNCEFEA NYVAIHTLAT WYKSNMNGVR KDVVMTDSED STVSIQIKLE 

       370        380        390        400        410        420 
NEGSDEDIET DVLYSPQMAL KLALTEWLQE FGVPHQYSSR QVAHSGAKAS VVDGTPLVAA 

       430        440        450        460        470        480 
PSLNATTVVT TVYQEPIMSQ GAALSGEPTT LTKEEEKKQP DEEPMDMVVE KQEETDHKND 

       490        500        510        520        530        540 
NQILSEIVEA KMAEELKPMD TDKESIAESK SPEMSMQEDC ISDIAPMQTD EQTNKEQFVP 

       550        560        570        580        590        600 
GPNEKPLYTA EPVTLEDLQL LADLFYLPYE HGPKGAQMLR EFQWLRANSS VVSVNCKGKD 

       610        620        630        640        650        660 
SEKIEEWRSR AAKFEEMCGL VMGMFTRLSN CANRTILYDM YSYVWDIKSI MSMVKSFVQW 

       670        680        690        700        710        720 
LGCRSHSSAQ FLIGDQEPWA FRGGLAGEFQ RLLPIDGAND LFFQPPPLTP TSKVYTIRPY 

       730        740        750        760        770        780 
FPKDEASVYK ICREMYDDGV GLPFQSQPDL IGDKLVGGLL SLSLDYCFVL EDEDGICGYA 

       790        800        810        820        830        840 
LGTVDVTPFI KKCKISWIPF MQEKYTKPNG DKELSEAEKI MLSFHEEQEV LPETFLANFP 

       850        860        870        880        890        900 
SLIKMDIHKK VTDPSVAKSM MACLLSSLKA NGSRGAFCEV RPDDKRILEF YSKLGCFEIA 

       910 
KMEGFPKDVV ILGRSL

« Hide

Isoform 2 [UniParc].

Checksum: A65EFD64BE0AD17C
Show »

84995,331
Isoform 3 (MGEA5s) [UniParc].

Checksum: BF83DEF3ABDE4AA8
Show »

67776,193

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References

« Hide 'large scale' references
[1]"Novel immunogenic antigen homologous to hyaluronidase in meningioma."
Heckel D., Comtesse N., Brass N., Blin N., Zang K.D., Meese E.
Hum. Mol. Genet. 7:1859-1872(1998) [PubMed: 9811929] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 3).
Tissue: Meningioma.
[2]"Identification of a nuclear variant of MGEA5, a cytoplasmic hyaluronidase and a beta-N-acetylglucosaminidase."
Comtesse N., Maldener E., Meese E.
Biochem. Biophys. Res. Commun. 283:634-640(2001) [PubMed: 11341771] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1 AND 3), SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[3]"Dynamic O-glycosylation of nuclear and cytosolic proteins: cloning and characterization of a neutral, cytosolic beta-N-acetylglucosaminidase from human brain."
Gao Y., Wells L., Comer F.I., Parker G.J., Hart G.W.
J. Biol. Chem. 276:9838-9845(2001) [PubMed: 11148210] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, IDENTIFICATION BY MASS SPECTROMETRY.
Tissue: Brain.
[4]"Prediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro."
Ishikawa K., Nagase T., Suyama M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
DNA Res. 5:169-176(1998) [PubMed: 9734811] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[5]"Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones."
Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T.
DNA Res. 9:99-106(2002) [PubMed: 12168954] [Abstract]
Cited for: SEQUENCE REVISION.
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT LYS-602.
Tissue: Cervix, Eye and Skin.
[7]"Dynamic O-glycosylation of nuclear and cytosolic proteins: further characterization of the nucleocytoplasmic beta-N-acetylglucosaminidase, O-GlcNAcase."
Wells L., Gao Y., Mahoney J.A., Vosseller K., Chen C., Rosen A., Hart G.W.
J. Biol. Chem. 277:1755-1761(2002) [PubMed: 11788610] [Abstract]
Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, PTM, SUBCELLULAR LOCATION.
[8]"Large-scale characterization of HeLa cell nuclear phosphoproteins."
Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-364, MASS SPECTROMETRY.
Tissue: Epithelium.
+Additional computationally mapped references.

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Cross-references

Sequence databases

AF036144 mRNA. Translation: AAD05385.2.
AF307332 mRNA. Translation: AAG21428.1.
AB014579 mRNA. Translation: BAA31654.2. Different initiation.
BC001343 mRNA. Translation: AAH01343.1.
BC039583 mRNA. Translation: AAH39583.2.
BC047877 mRNA. Translation: AAH47877.1. Sequence problems.
PIRT00360.
RefSeqNP_036347.1.
UniGeneHs.500842

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

IntActO60502.

PTM databases

PhosphoSiteO60502.

Genome annotation databases

EnsemblENSG00000198408. Homo sapiens. [Contig view]
GeneID10724.
KEGGhsa:10724.

Organism-specific databases

H-InvDBHIX0009144.
HGNCHGNC:7056. MGEA5.
MIM604039. gene.
PharmGKBPA30787.
HUGESearch...
GenAtlasSearch...
GeneCardsSearch...

Phylogenomic databases

HOVERGENO60502.

Gene expression databases

ArrayExpressO60502.
CleanExHS_MGEA5.
GermOnlineENSG00000198408. Homo sapiens.

Family and domain databases

InterProIPR016181. Acyl_CoA_acyltransferase.
IPR011496. Hyal_ase.
[Graphical view]
Gene3DG3DSA:3.40.630.30. Acyl_CoA_acyltransferase. 1 hit.
PfamPF07555. Hyaluronidase_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio40709.
SOURCESearch...

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Entry information

Entry nameNCOAT_HUMAN
AccessionPrimary (citable) accession number: O60502
Secondary accession number(s): O75166 expand/collapse secondary AC list , Q86WV0, Q8IV98, Q9BVA5, Q9HAR0
Entry history
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: March 1, 2001
Last modified: November 25, 2008
This is version 47 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

Human chromosome 10

Human chromosome 10: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents