Reviewed, UniProtKB/Swiss-Prot Q86XK2 (FBX11_HUMAN)

Names and origin

Protein names

Recommended name:
    F-box only protein 11
Alternative name(s):
    Vitiligo-associated protein 1
      Short name=VIT-1

Gene names

Name:	FBXO11
Synonyms:	FBX11, VIT1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	927 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Substrate recognition component of the a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins. Binds to and neddylates phosphorylated TP53/p53, inhibiting its transcriptional activity. SCF(FBXO11) does not seem to direct ubiquitination of TP53.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Component of the probable SCF(FBXWO11) complex consisting of CUL1, RBX1, SKP1A and FBXO11. Interacts with TP53.
Subcellular location	Nucleus.
Tissue specificity	Isoform 5 is expressed in keratinocytes, fibroblasts and melanocytes.
Involvement in disease	FBXO11 is down-regulated in vitiligo [MIM:193200]. It is a skin disorder associated with progressive skin depigmentation.
Sequence similarities	Contains 1 F-box domain. Contains 19 PbH1 repeats. Contains 1 UBR-type zinc finger.
Sequence caution	The sequence AAF76888.1 differs from that shown. Reason: Miscellaneous discrepancy. Intron retention. The sequence AAN76518.1 differs from that shown. Reason: Frameshift at position 141.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
Gene Ontology (GO)
Biological process	protein ubiquitination Ref.5 Non-traceable author statement. Source: UniProtKB ubiquitin-dependent protein catabolic process Ref.7 Non-traceable author statement. Source: UniProtKB
Cellular component	cytoplasm Inferred from direct assay. Source: HGNC nucleus Inferred from direct assay. Source: HGNC ubiquitin ligase complex Ref.5 Ref.7 Non-traceable author statement. Source: UniProtKB
Molecular function	histone-arginine N-methyltransferase activity Inferred from direct assay. Source: HGNC peptidyl-arginine C-methyltransferase activity Inferred from direct assay. Source: HGNC protein binding Ref.1 Inferred from physical interaction. Source: IntAct ubiquitin-protein ligase activity Ref.5 Non-traceable author statement. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
TP53	P04637	3	EBI-1047804,EBI-366083

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q86XK2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q86XK2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-209: Missing.
     744-770: LLEENDIFRNAQAGVLISTNSHPILRK → IVVNFALVKNPVFHYSSISLMINDIAN
     771-927: Missing.

Notes: No experimental confirmation available.

Isoform 3 (identifier: Q86XK2-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-84: Missing.
     670-927: Missing.

Notes: No experimental confirmation available.

Isoform 4 (identifier: Q86XK2-4)

The sequence of this isoform differs from the canonical sequence as follows:
     197-211: KRLYMEVFEYTRPMM → LGEVAHAYNPSTLGG
     212-927: Missing.

Isoform 5 (identifier: Q86XK2-5)

The sequence of this isoform differs from the canonical sequence as follows:
     885-885: R → RYVAHLLDILPNYFPPHFSNIWVSFCFR

Notes: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 927	927	F-box only protein 11
Regions
	Domain	153 – 199	47	F-box
	Repeat	395 – 417	23	PbH1 1
	Repeat	418 – 440	23	PbH1 2
	Repeat	441 – 463	23	PbH1 3
	Repeat	464 – 486	23	PbH1 4
	Repeat	487 – 509	23	PbH1 5
	Repeat	510 – 532	23	PbH1 6
	Repeat	533 – 555	23	PbH1 7
	Repeat	556 – 578	23	PbH1 8
	Repeat	579 – 601	23	PbH1 9
	Repeat	602 – 624	23	PbH1 10
	Repeat	625 – 647	23	PbH1 11
	Repeat	648 – 670	23	PbH1 12
	Repeat	671 – 693	23	PbH1 13
	Repeat	694 – 716	23	PbH1 14
	Repeat	717 – 739	23	PbH1 15
	Repeat	740 – 762	23	PbH1 16
	Repeat	763 – 785	23	PbH1 17
	Repeat	786 – 808	23	PbH1 18
	Repeat	809 – 830	22	PbH1 19
	Zinc finger	833 – 904	72	UBR-type
Natural variations
	Alternative sequence	1 – 209	209	Missing in isoform 2.
	Alternative sequence	1 – 84	84	Missing in isoform 3.
	Alternative sequence	197 – 211	15	KRLYM…TRPMM → LGEVAHAYNPSTLGG in isoform 4.
	Alternative sequence	212 – 927	716	Missing in isoform 4.
	Alternative sequence	670 – 927	258	Missing in isoform 3.
	Alternative sequence	744 – 770	27	LLEEN…PILRK → IVVNFALVKNPVFHYSSISL MINDIAN in isoform 2.
	Alternative sequence	771 – 927	157	Missing in isoform 2.
	Alternative sequence	885	1	R → RYVAHLLDILPNYFPPHFSN IWVSFCFR in isoform 5.
	Natural variant	126	1	T → S: dbSNP rs17036993.
Experimental info
	Sequence conflict	239	1	L → S in BAB14214. Ref.2
	Sequence conflict	577	1	R → G in AAN76518. Ref.3
	Sequence conflict	632	1	R → G in BAD97312. Ref.6
	Sequence conflict	903	1	G → S in AAV87312. Ref.1
	Sequence conflict	903	1	G → S in AAH43258. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: CADBD23A6B892A78	FASTA	927	103,585
	10 20 30 40 50 60 MNSVRAANRR PRRVSRPRPV QQQQQQPPQQ PPPQPPQQQP PQQQPPPPPQ QQQQQQPPPP 70 80 90 100 110 120 PPPPPPLPQE RNNVGERDDD VPADMVAEES GPGAQNSPYQ LRRKTLLPKR TACPTKNSME 130 140 150 160 170 180 GASTSTTENF GHRAKRARVS GKSQDLSAAP AEQYLQEKLP DEVVLKIFSY LLEQDLCRAA 190 200 210 220 230 240 CVCKRFSELA NDPILWKRLY MEVFEYTRPM MHPEPGKFYQ INPEEYEHPN PWKESFQQLY 250 260 270 280 290 300 KGAHVKPGFA EHFYSNPARY KGRENMLYYD TIEDALGGVQ EAHFDGLIFV HSGIYTDEWI 310 320 330 340 350 360 YIESPITMIG AAPGKVADKV IIENTRDSTF VFMEGSEDAY VGYMTIRFNP DDKSAQHHNA 370 380 390 400 410 420 HHCLEITVNC SPIIDHCIIR STCTVGSAVC VSGQGACPTI KHCNISDCEN VGLYITDHAQ 430 440 450 460 470 480 GIYEDNEISN NALAGIWVKN HGNPIIRRNH IHHGRDVGVF TFDHGMGYFE SCNIHRNRIA 490 500 510 520 530 540 GFEVKAYANP TVVRCEIHHG QTGGIYVHEK GRGQFIENKI YANNFAGVWI TSNSDPTIRG 550 560 570 580 590 600 NSIFNGNQGG VYIFGDGRGL IEGNDIYGNA LAGIQIRTNS CPIVRHNKIH DGQHGGIYVH 610 620 630 640 650 660 EKGQGVIEEN EVYSNTLAGV WVTTGSTPVL RRNRIHSGKQ VGVYFYDNGH GVLEDNDIYN 670 680 690 700 710 720 HMYSGVQIRT GSNPKIRRNK IWGGQNGGIL VYNSGLGCIE DNEIFDNAMA GVWIKTDSNP 730 740 750 760 770 780 TLRRNKIHDG RDGGICIFNG GRGLLEENDI FRNAQAGVLI STNSHPILRK NRIFDGFAAG 790 800 810 820 830 840 IEITNHATAT LEGNQIFNNR FGGLFLASGV NVTMKDNKIM NNQDAIEKAV SRGQCLYKIS 850 860 870 880 890 900 SYTSYPMHDF YRCHTCNTTD RNAICVNCIK KCHQGHDVEF IRHDRFFCDC GAGTLSNPCT 910 920 LAGEPTHDTD TLYDSAPPIE SNTLQHN
	Isoform 2 [UniParc]. Checksum: 53E2FF0C642059C6		561	62,418
	10 20 30 40 50 60 MMHPEPGKFY QINPEEYEHP NPWKESFQQL YKGAHVKPGF AEHFYSNPAR YKGRENMLYY 70 80 90 100 110 120 DTIEDALGGV QEAHFDGLIF VHSGIYTDEW IYIESPITMI GAAPGKVADK VIIENTRDST 130 140 150 160 170 180 FVFMEGSEDA YVGYMTIRFN PDDKSAQHHN AHHCLEITVN CSPIIDHCII RSTCTVGSAV 190 200 210 220 230 240 CVSGQGACPT IKHCNISDCE NVGLYITDHA QGIYEDNEIS NNALAGIWVK NHGNPIIRRN 250 260 270 280 290 300 HIHHGRDVGV FTFDHGMGYF ESCNIHRNRI AGFEVKAYAN PTVVRCEIHH GQTGGIYVHE 310 320 330 340 350 360 KGRGQFIENK IYANNFAGVW ITSNSDPTIR GNSIFNGNQG GVYIFGDGRG LIEGNDIYGN 370 380 390 400 410 420 ALAGIQIRTN SCPIVRHNKI HDGQHGGIYV HEKGQGVIEE NEVYSNTLAG VWVTTGSTPV 430 440 450 460 470 480 LRRNRIHSGK QVGVYFYDNG HGVLEDNDIY NHMYSGVQIR TGSNPKIRRN KIWGGQNGGI 490 500 510 520 530 540 LVYNSGLGCI EDNEIFDNAM AGVWIKTDSN PTLRRNKIHD GRDGGICIFN GGRGIVVNFA 550 560 LVKNPVFHYS SISLMINDIA N
	Isoform 3 [UniParc]. Checksum: 319A266C66D541B6		585	65,559
	10 20 30 40 50 60 MVAEESGPGA QNSPYQLRRK TLLPKRTACP TKNSMEGAST STTENFGHRA KRARVSGKSQ 70 80 90 100 110 120 DLSAAPAEQY LQEKLPDEVV LKIFSYLLEQ DLCRAACVCK RFSELANDPI LWKRLYMEVF 130 140 150 160 170 180 EYTRPMMHPE PGKFYQINPE EYEHPNPWKE SFQQLYKGAH VKPGFAEHFY SNPARYKGRE 190 200 210 220 230 240 NMLYYDTIED ALGGVQEAHF DGLIFVHSGI YTDEWIYIES PITMIGAAPG KVADKVIIEN 250 260 270 280 290 300 TRDSTFVFME GSEDAYVGYM TIRFNPDDKS AQHHNAHHCL EITVNCSPII DHCIIRSTCT 310 320 330 340 350 360 VGSAVCVSGQ GACPTIKHCN ISDCENVGLY ITDHAQGIYE DNEISNNALA GIWVKNHGNP 370 380 390 400 410 420 IIRRNHIHHG RDVGVFTFDH GMGYFESCNI HRNRIAGFEV KAYANPTVVR CEIHHGQTGG 430 440 450 460 470 480 IYVHEKGRGQ FIENKIYANN FAGVWITSNS DPTIRGNSIF NGNQGGVYIF GDGRGLIEGN 490 500 510 520 530 540 DIYGNALAGI QIRTNSCPIV RHNKIHDGQH GGIYVHEKGQ GVIEENEVYS NTLAGVWVTT 550 560 570 580 GSTPVLRRNR IHSGKQVGVY FYDNGHGVLE DNDIYNHMYS GVQIR
	Isoform 4 [UniParc]. Checksum: EA62852C6C1C91E3		211	23,441
	10 20 30 40 50 60 MNSVRAANRR PRRVSRPRPV QQQQQQPPQQ PPPQPPQQQP PQQQPPPPPQ QQQQQQPPPP 70 80 90 100 110 120 PPPPPPLPQE RNNVGERDDD VPADMVAEES GPGAQNSPYQ LRRKTLLPKR TACPTKNSME 130 140 150 160 170 180 GASTSTTENF GHRAKRARVS GKSQDLSAAP AEQYLQEKLP DEVVLKIFSY LLEQDLCRAA 190 200 210 CVCKRFSELA NDPILWLGEV AHAYNPSTLG G
	Isoform 5 [UniParc]. Checksum: 1F8E7073B253AC2D		954	106,864
	10 20 30 40 50 60 MNSVRAANRR PRRVSRPRPV QQQQQQPPQQ PPPQPPQQQP PQQQPPPPPQ QQQQQQPPPP 70 80 90 100 110 120 PPPPPPLPQE RNNVGERDDD VPADMVAEES GPGAQNSPYQ LRRKTLLPKR TACPTKNSME 130 140 150 160 170 180 GASTSTTENF GHRAKRARVS GKSQDLSAAP AEQYLQEKLP DEVVLKIFSY LLEQDLCRAA 190 200 210 220 230 240 CVCKRFSELA NDPILWKRLY MEVFEYTRPM MHPEPGKFYQ INPEEYEHPN PWKESFQQLY 250 260 270 280 290 300 KGAHVKPGFA EHFYSNPARY KGRENMLYYD TIEDALGGVQ EAHFDGLIFV HSGIYTDEWI 310 320 330 340 350 360 YIESPITMIG AAPGKVADKV IIENTRDSTF VFMEGSEDAY VGYMTIRFNP DDKSAQHHNA 370 380 390 400 410 420 HHCLEITVNC SPIIDHCIIR STCTVGSAVC VSGQGACPTI KHCNISDCEN VGLYITDHAQ 430 440 450 460 470 480 GIYEDNEISN NALAGIWVKN HGNPIIRRNH IHHGRDVGVF TFDHGMGYFE SCNIHRNRIA 490 500 510 520 530 540 GFEVKAYANP TVVRCEIHHG QTGGIYVHEK GRGQFIENKI YANNFAGVWI TSNSDPTIRG 550 560 570 580 590 600 NSIFNGNQGG VYIFGDGRGL IEGNDIYGNA LAGIQIRTNS CPIVRHNKIH DGQHGGIYVH 610 620 630 640 650 660 EKGQGVIEEN EVYSNTLAGV WVTTGSTPVL RRNRIHSGKQ VGVYFYDNGH GVLEDNDIYN 670 680 690 700 710 720 HMYSGVQIRT GSNPKIRRNK IWGGQNGGIL VYNSGLGCIE DNEIFDNAMA GVWIKTDSNP 730 740 750 760 770 780 TLRRNKIHDG RDGGICIFNG GRGLLEENDI FRNAQAGVLI STNSHPILRK NRIFDGFAAG 790 800 810 820 830 840 IEITNHATAT LEGNQIFNNR FGGLFLASGV NVTMKDNKIM NNQDAIEKAV SRGQCLYKIS 850 860 870 880 890 900 SYTSYPMHDF YRCHTCNTTD RNAICVNCIK KCHQGHDVEF IRHDRYVAHL LDILPNYFPP 910 920 930 940 950 HFSNIWVSFC FRFFCDCGAG TLSNPCTLAG EPTHDTDTLY DSAPPIESNT LQHN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"FBXO11 promotes the neddylation of p53 and inhibits its transcriptional activity." Abida W.M., Nikolaev A., Zhao W., Zhang W., Gu W. J. Biol. Chem. 282:1797-1804(2007) [PubMed: 17098746] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH TP53, IDENTIFICATION IN A COMPLEX WITH SKP1A; CUL1 AND RBX1.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 646-927 (ISOFORM 1). Tissue: Hepatoma and Teratocarcinoma.
[3]	"Isolation of full-length cDNA clones from human fetal brain cDNA library." Xie Y., Mao Y. Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3). Tissue: Fetal brain.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 15-927 (ISOFORM 1). Tissue: Ovary and Testis.
[5]	"Identification of a family of human F-box proteins." Cenciarelli C., Chiaur D.S., Guardavaccaro D., Parks W., Vidal M., Pagano M. Curr. Biol. 9:1177-1179(1999) [PubMed: 10531035] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 16-212.
[6]	Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 16-927 (ISOFORM 1). Tissue: Kidney.
[7]	"A family of mammalian F-box proteins." Winston J.T., Koepp D.M., Zhu C., Elledge S.J., Harper J.W. Curr. Biol. 9:1180-1182(1999) [PubMed: 10531037] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 20-927 (ISOFORM 4).
[8]	The German cDNA consortium Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 720-927. Tissue: Brain.
[9]	"'VIT1', a novel gene associated with vitiligo." Le Poole I.C., Sarangarajan R., Zhao Y., Stennett L.S., Brown T.L., Sheth P., Miki T., Boissy R.E. Pigment Cell Res. 14:475-484(2001) [PubMed: 11775060] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 744-927 (ISOFORM 5), TISSUE SPECIFICITY, DISEASE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY827075 mRNA. Translation: AAV87312.1. AK022735 mRNA. Translation: BAB14214.1. AK025477 mRNA. Translation: BAB15143.1. Different initiation. AF351618 mRNA. Translation: AAN76518.1. Sequence problems. BC012728 mRNA. Translation: AAH12728.2. BC043258 mRNA. Translation: AAH43258.2. AF174599 mRNA. Translation: AAF04520.1. AK223592 mRNA. Translation: BAD97312.1. Different initiation. AF176706 mRNA. Translation: AAF17611.1. AL117620 mRNA. Translation: CAB56019.1. AF264714 mRNA. Translation: AAF76888.1. Sequence problems.
PIR	T17329.
RefSeq	NP_036299.1. NP_061163.2. NP_079409.3.
UniGene	Hs.352677
3D structure databases
HSSP	HSSP built from PDB template 1FS1 based on UniProtKB Q13309.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q86XK2.
Genome annotation databases
Ensembl	ENSG00000138081. Homo sapiens. [Contig view]
GeneID	80204.
KEG