Reviewed, UniProtKB/Swiss-Prot Q896F2 (SYA_CLOTE)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	CTC_01055

Organism

Clostridium tetani [Complete proteome] [HAMAP]

Taxonomic identifier

1513 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	879 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 879	879	Alanyl-tRNA synthetase

Sequences

	Sequence		Length	Mass (Da)
	Q896F2-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: D515A821F7524E10	FASTA	879	98,457
	10 20 30 40 50 60 MKNMGLNEIR EAYLNFFEGK GHLRMESFSL VPKNDKSLLL INAGMAPLKP YFTGVQTPPK 70 80 90 100 110 120 SRVTTCQKCI RTGDIENVGK TSRHGTFFEM LGNFSFGDYF KCEVIPWAWE FVTKSLNIPK 130 140 150 160 170 180 DKLYVTIYLD DDEAYDIWTK STDIDPSRIF RLGKEDNFWE IGQGPCGPCS EIHYQREGEK 190 200 210 220 230 240 INSVEEFIKK SDDDEVIEFW NLVFTQFDKD ENENYNRLAH PNIDTGMGLE RMATIMQGVN 250 260 270 280 290 300 SIFEVDTIKA VLDEISEIAN IKYGENKEKD ISLRVITDHV RSVTFMISDG ILPSNEGRGY 310 320 330 340 350 360 VLRRLLRRAS RHGKLLGIKG NFLYKVCKVV IENSHKAYKE LKEKEEYIKK IIKLEEERFA 370 380 390 400 410 420 ETIDGGIQIL NEYVDELINK GEKVLPGDKA FKLYDTYGFP IELTKEILEE KSIDIDEGGF 430 440 450 460 470 480 TKEMEAQKQR ARAAREETNY MGSEDTIINK LPIELQTEFL GYSELSVEAK IIAIIKGEEL 490 500 510 520 530 540 VEELKQGDKG IIIVDKTPFY SEKGGQIGDT GILAGKNVKV KIQDCRNNIS GKILHFVEIL 550 560 570 580 590 600 EGSIKLQDTV NLTVDRLRRD AIRKNHSATH LLHEALKKIV GAHIEQAGSF VDEHRLRFDF 610 620 630 640 650 660 NHFSSLTKEE LKNVEKLVNK KIMEVIPVNT KVMNIEEAKE SGAVALFDEK YDEKVRVVSL 670 680 690 700 710 720 GDFSKELCGG THVSNSGEIG LFKIISETGV AAGVRRIEGV TGFNALKYIE EKEALLEGLC 730 740 750 760 770 780 EVLKCSNKDI INKATSQIEE IKNKEKEINN LKSKLASGSQ EDILKNIKEV KGIKLVSGVL 790 800 810 820 830 840 KDIDGGALRD LADKLRDKIQ EGLVVLASVG EGKIQFVAMA TKEAVAKGAH CGKIIKEVAS 850 860 870 IAGGGGGGRP DMAQAGGKNP EKAEEAIAKV EDILSSLVK

References

[1]

"The genome sequence of Clostridium tetani, the causative agent of tetanus disease." Brueggemann H., Baeumer S., Fricke W.F., Wiezer A., Liesegang H., Decker I., Herzberg C., Martinez-Arias R., Merkl R., Henne A., Gottschalk G. Proc. Natl. Acad. Sci. U.S.A. 100:1316-1321(2003) [PubMed: 12552129] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Massachusetts / E88.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015927 Genomic DNA. Translation: AAO35638.1.
RefSeq	NP_781701.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1060244.
GenomeReviews	Gene locus CTC_01055 in contig AE015927_GR.
KEGG	ctc:CTC01055.
NMPDR	fig|212717.1.peg.923.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q896F2.
Enzyme and pathway databases
BioCyc	CTET212717:CTC_01055-MON.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProDom	Q896F2. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

SYA_CLOTE

Accession

Primary (citable) accession number: Q896F2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	June 1, 2003
Last modified:	July 22, 2008
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)