Reviewed, UniProtKB/Swiss-Prot Q89I89 (SYA_BRAJA)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name(s)=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	bll5750

Organism

Bradyrhizobium japonicum [Complete proteome] [HAMAP]

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	894 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 894	894	Alanyl-tRNA synthetase

Sequences

	Sequence		Length	Mass (Da)
	Q89I89-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: E292AF91647507E5	FASTA	894	97,018
	10 20 30 40 50 60 MNMSGVNEIR STFLNFFAEN GHEIVPSSPL VPRNDPTLMF TNAGMVQFKN VFTGVEKRPY 70 80 90 100 110 120 QRATTSQKCV RAGGKHNDLD NVGYTARHLT FFEMLGNFSF GDYFKERAIE LAWKLITKDF 130 140 150 160 170 180 GLNKDKLLVT VYHTDDEAHG LWKKIAGFSD DRIIRIPTSD NFWAMGDTGP CGPCSEIFID 190 200 210 220 230 240 RGDHIWGGPP GSPEEDGDRF LEFWNLVFMQ YEQVTKEERV DLPRPSIDTG MGLERMACIM 250 260 270 280 290 300 QGVDSVFETD LFRHLIDATA SALGSGPNEQ TVASFRVIAD HLRSSAFLVS DGVLPSNEGR 310 320 330 340 350 360 GYVLRRIMRR AMRHAQLLGA KEPLMHRLVW ALVREMGQAY PELMRAEKLI EETLRLEETR 370 380 390 400 410 420 FRKTLTRGLA ILDEKSASLK KGDMFDGDVA FTLYDTYGFP LDLTQDALKS RGIGVDQSAF 430 440 450 460 470 480 TDAMERQKAK ARESWKGSGE AASEAIWFPL REKLGATEFL GYETESAEGV VSALVKDGQE 490 500 510 520 530 540 AASLKAGETG ALVLNQTPFY AESGGQVGDT GVLLGEGGVK FRVTDTQKKL GDLFVHVGTV 550 560 570 580 590 600 ESGELKLGTA LQLEVDHSRR SSIRAHHSAT HLIHEALRQV LGDHIAQRGS MVAPDRLRFD 610 620 630 640 650 660 FVHPKPITAE ELARVEDIAN DVVLENDEVT TRVMGVDEAR EAGARALFGE KYGDEVRVVS 670 680 690 700 710 720 MGRTARERGA NALGWSVELC GGTHVRRTGD IGLITLTGES AVASGVRRIE ALTGNYARKH 730 740 750 760 770 780 ANDTMALAKT AANELRTSID DVPARITALM EERKKLEREL SDARKKLAMG GGAAASNGAA 790 800 810 820 830 840 SGVREVGDVK LMARAVEGIE MKDLKSLADD GKKQIGSGVV AIVGVTGDGK AGIVVGVTAD 850 860 870 880 890 LTARFNAVNL VRVASEALGG KGGGGRPDMA QAGGPDGAKA PEALAAIEKA MAGA

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000040 Genomic DNA. Translation: BAC51015.1.
RefSeq	NP_772390.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1048553.
GenomeReviews	Gene locus bll5750 in contig BA000040_GR.
KEGG	bja:bll5750.
NMPDR	fig|224911.1.peg.5750.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q89I89.
Enzyme and pathway databases
BioCyc	BJAP224911:BLL5750-MON.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProDom	Q89I89. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

SYA_BRAJA

Accession

Primary (citable) accession number: Q89I89

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	June 1, 2003
Last modified:	July 22, 2008
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)