Reviewed, UniProtKB/Swiss-Prot Q8BWW9 (PKN2_MOUSE)

Names and origin

Protein names

Recommended name:
    Serine/threonine-protein kinase N2
    EC=2.7.11.13
Alternative name(s):
    Protein kinase C-like 2
    Protein-kinase C-related kinase 2

Gene names

Name:	Pkn2
Synonyms:	Prkcl2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	983 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Exhibits a preference for highly basic protein substrates By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Activated by lipids, particularly cardiolipin and to a lesser extent by other acidic phospholipids and unsaturated fatty acids. Two specific sites, Thr-815 (activation loop of the kinase domain) and Thr-957 (turn motif), need to be phosphorylated for its full activation By similarity.
Subcellular location	CytoplasmBy similarity.
Domain	The C1 domain does not bind the diacylglycerol (DAG).
Post-translational modification	Autophosphorylated. Activated by limited proteolysis with trypsin By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 C2 domain. Contains 1 protein kinase domain. Contains 3 REM (Hr1) repeats.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from direct assay. Source: MGI nucleus Inferred from direct assay. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8BWW9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8BWW9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     35-45: Missing.

Notes: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 983	983	Serine/threonine-protein kinase N2
Regions
	Repeat	44 – 119	76	REM 1
	Repeat	133 – 213	81	REM 2
	Repeat	214 – 295	82	REM 3
	Domain	329 – 462	134	C2
	Domain	656 – 915	260	Protein kinase
	Domain	916 – 983	68	AGC-kinase C-terminal
	Nucleotide binding	662 – 670	9	ATP By similarity
Sites
	Active site	781	1	Proton acceptor By similarity
	Binding site	685	1	ATP By similarity
Amino acid modifications
	Modified residue	289	1	Phosphoserine By similarity
	Modified residue	301	1	Phosphoserine By similarity
	Modified residue	305	1	Phosphoserine By similarity
	Modified residue	359	1	Phosphoserine By similarity
	Modified residue	530	1	Phosphoserine By similarity
	Modified residue	534	1	Phosphoserine By similarity
	Modified residue	558	1	Phosphoserine By similarity
	Modified residue	560	1	Phosphoserine By similarity
	Modified residue	581	1	Phosphoserine By similarity
	Modified residue	582	1	Phosphoserine
	Modified residue	815	1	Phosphothreonine By similarity
	Modified residue	957	1	Phosphothreonine Probable
Natural variations
	Alternative sequence	35 – 45	11	Missing in isoform 2.
Experimental info
	Sequence conflict	84	1	H → N in AAH52073. Ref.2
	Sequence conflict	395	1	V → D in BAC38910. Ref.1
	Sequence conflict	704	1	E → K in BAC33888. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 23, 2004. Version 2. Checksum: 22422CBE364EDCD7	FASTA	983	111,630
	10 20 30 40 50 60 MASNPDRGEI LLTELQGDSR TLPFSENVSA VQKLDFSDTM VQQKLDDIKD RIKREIRKEL 70 80 90 100 110 120 KIKEGAENLR KVTTDKKNLA YVDHILKKSN KKLEELHHKL QELNAHIVVS DPEDSTDCPR 130 140 150 160 170 180 TPDTPNSDSR SSTSNNRLMA LQKQLDIELK VKQGAENMIQ MYSNGSSKDR KLHGTAQQLL 190 200 210 220 230 240 QDSKTKIEVI RMQILQAVQT NELAFDNAKP VISPLELRME ELRHHFKIEF AVAEGAKNVM 250 260 270 280 290 300 KLLGSGKVTD RKALSEAQAR FNESSQKLDL LKYSLEQRLN ELPRNHPKSS VVIEELSLVA 310 320 330 340 350 360 SPTLSPRQSM LSTQNQYSTL SKPAALTGTL EVRLMGCQDI LENVPGRSKA TSVALPGWSP 370 380 390 400 410 420 SDNRSSFMSR TSKSKSGSSR NLLKTDDLSN DVCAVLKLDN TVVGQTSWKP ISNQSWDQKF 430 440 450 460 470 480 TLELDRSREL EISVYWRDWR SLCAVKFLRL EDFLDNQRHG MCLYLEPQGT LFAEVTFFNP 490 500 510 520 530 540 VIERRPKLQR QKKIFSKQQG KTFLRAPQMN INIATWGRLV RRAIPTVNHS GTFSPQTPVP 550 560 570 580 590 600 ATVPVVDARI PDLAPPASDS TVTKLDFDLE PEPPPAPPRA SSLGETDESS ELRVLDIPGQ 610 620 630 640 650 660 GSETVFNIEN DRNNLRPKSK SEYELSIPDS GRSCWGVGEL DDKRAQQRFQ FSLQDFRCCA 670 680 690 700 710 720 VLGRGHFGKV LLAEYKHTNE MFAIKALKKG DIVARDEVDS LMCEKRIFET VNSVRHPFLV 730 740 750 760 770 780 NLFACFQTKE HVCFVMEYAA GGDLMMHIHT DVFSEPRAVF YAACVVLGLQ YLHEHKIVYR 790 800 810 820 830 840 DLKLDNLLLD TEGFVKIADF GLCKEGMGYG DRTSTFCGTP EFLAPEVLTE TSYTRAVDWW 850 860 870 880 890 900 GLGVLIYEML VGESPFPGDD EEEVFDSIVN DEVRYPRFLS TEAISIMRRL LRRNPERRLG 910 920 930 940 950 960 AGEKDAEDVK KHPFFRLTDW SALMDKKVKP PFVPTIRGRE DVSNFDDEFT SEAPILTPPR 970 980 EPRILLEEEQ EMFHDFDYVA DWC
	Isoform 2 [UniParc]. Checksum: C9A3BA0A953341F7		972	110,337
	10 20 30 40 50 60 MASNPDRGEI LLTELQGDSR TLPFSENVSA VQKLDDIKDR IKREIRKELK IKEGAENLRK 70 80 90 100 110 120 VTTDKKNLAY VDHILKKSNK KLEELHHKLQ ELNAHIVVSD PEDSTDCPRT PDTPNSDSRS 130 140 150 160 170 180 STSNNRLMAL QKQLDIELKV KQGAENMIQM YSNGSSKDRK LHGTAQQLLQ DSKTKIEVIR 190 200 210 220 230 240 MQILQAVQTN ELAFDNAKPV ISPLELRMEE LRHHFKIEFA VAEGAKNVMK LLGSGKVTDR 250 260 270 280 290 300 KALSEAQARF NESSQKLDLL KYSLEQRLNE LPRNHPKSSV VIEELSLVAS PTLSPRQSML 310 320 330 340 350 360 STQNQYSTLS KPAALTGTLE VRLMGCQDIL ENVPGRSKAT SVALPGWSPS DNRSSFMSRT 370 380 390 400 410 420 SKSKSGSSRN LLKTDDLSND VCAVLKLDNT VVGQTSWKPI SNQSWDQKFT LELDRSRELE 430 440 450 460 470 480 ISVYWRDWRS LCAVKFLRLE DFLDNQRHGM CLYLEPQGTL FAEVTFFNPV IERRPKLQRQ 490 500 510 520 530 540 KKIFSKQQGK TFLRAPQMNI NIATWGRLVR RAIPTVNHSG TFSPQTPVPA TVPVVDARIP 550 560 570 580 590 600 DLAPPASDST VTKLDFDLEP EPPPAPPRAS SLGETDESSE LRVLDIPGQG SETVFNIEND 610 620 630 640 650 660 RNNLRPKSKS EYELSIPDSG RSCWGVGELD DKRAQQRFQF SLQDFRCCAV LGRGHFGKVL 670 680 690 700 710 720 LAEYKHTNEM FAIKALKKGD IVARDEVDSL MCEKRIFETV NSVRHPFLVN LFACFQTKEH 730 740 750 760 770 780 VCFVMEYAAG GDLMMHIHTD VFSEPRAVFY AACVVLGLQY LHEHKIVYRD LKLDNLLLDT 790 800 810 820 830 840 EGFVKIADFG LCKEGMGYGD RTSTFCGTPE FLAPEVLTET SYTRAVDWWG LGVLIYEMLV 850 860 870 880 890 900 GESPFPGDDE EEVFDSIVND EVRYPRFLST EAISIMRRLL RRNPERRLGA GEKDAEDVKK 910 920 930 940 950 960 HPFFRLTDWS ALMDKKVKPP FVPTIRGRED VSNFDDEFTS EAPILTPPRE PRILLEEEQE 970 MFHDFDYVAD WC

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J. Tissue: Spinal cord.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: C57BL/6. Tissue: Brain.
[3]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed: 17242355] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-582, MASS SPECTROMETRY. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AK049713 mRNA. Translation: BAC33888.1. AK083425 mRNA. Translation: BAC38910.1. BC052073 mRNA. Translation: AAH52073.1.
RefSeq	NP_848769.1.
UniGene	Mm.244236 Mm.440835
3D structure databases
HSSP	HSSP built from PDB template 1URF based on UniProtKB Q16512.
ModBase	Search...
PTM databases
PhosphoSite	Q8BWW9.
Genome annotation databases
Ensembl	ENSMUSG00000004591. Mus musculus. [Contig view]
GeneID	109333.
KEGG	mmu:109333.
Organism-specific databases
MGI	MGI:109211. Pkn2.
Phylogenomic databases
HOGENOM	Q8BWW9.
HOVERGEN	Q8BWW9.
Gene expression databases
ArrayExpress	Q8BWW9.
CleanEx	MM_PKN2.
GermOnline	ENSMUSG00000004591. Mus musculus.
Family and domain databases
InterPro	IPR000008. C2_Ca-dep. IPR000861. HR1-like_rho-bd. IPR011072. HR1_rho-bd. IPR000961. Pkinase_C. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_bd_CS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Gene3D	G3DSA:1.10.287.160. HR1_rho-bd. 3 hits.
Pfam	PF02185. HR1. 3 hits. PF00069. Pkinase. 1 hit. PF00433. Pkinase_C. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00239. C2. 1 hit. SM00742. Hr1. 3 hits. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS50004. C2. False negative. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
BLOCKS	Search...
Other Resources
SOURCE	Search...
ProtoNet	Search...

Entry information

Entry name

PKN2_MOUSE

Accession

Primary (citable) accession number: Q8BWW9
Secondary accession number(s): Q80WS2, Q8BJL7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 23, 2004
Last sequence update:	November 23, 2004
Last modified:	July 22, 2008
This is version 56 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)