Reviewed, UniProtKB/Swiss-Prot Q8CFX8 (SYAC_MESAU)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase, cytoplasmic
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name(s)=AlaRS

Gene names

Name:

AARS

Organism

Mesocricetus auratus (Golden hamster)

Taxonomic identifier

10036 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Mesocricetus

Protein attributes

Sequence length	968 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).
Subunit structure	Monomer By similarity.
Subcellular location	CytoplasmPotential.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Coding sequence diversity	Polymorphism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 968	968	Alanyl-tRNA synthetase, cytoplasmic
Regions
	Region	1 – 497	497	Catalytic By similarity
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine By similarity
	Modified residue	578	1	Phosphothreonine By similarity
	Modified residue	580	1	Phosphotyrosine By similarity
Natural variations
	Natural variant	321	1	G → R in ts ET12; a temperature-sensitive mutant; cells undergo apoptosis 48 hours after a temperature-shift to 39.5 degrees Celsius.

Sequences

	Sequence		Length	Mass (Da)
	Q8CFX8-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 20490C36A57321E6	FASTA	968	106,714
	10 20 30 40 50 60 MDSPLTAREI RERFINFFRR NEHTYVHSSA TIPLDDPTLL FANAGMNQFK PIFLNTIDPS 70 80 90 100 110 120 HPLAKLSRAA NTQKCIRAGG KHNDLDDVGK DVYHHTFFEM LGSWSFGDYF KELACKMALE 130 140 150 160 170 180 LLTQEFAIPV ERLYVTYFGG DEAAGLEPDL ECKQIWQNLG LDDAKILPGN MKDNFWEMGD 190 200 210 220 230 240 TGPCGPCSEI HYDRIGGRDA AHLVNQDDPN VLEIWNLVFI QYNRETDGLL KPLPKKSIDT 250 260 270 280 290 300 GMGLERLVSV LQNKMSNYDT DLFVPYFEAI QKGTGARPYT GKVGAEDADG IDMAYRVLAD 310 320 330 340 350 360 HARTITVALA DGGRPDNTGR GYVLRRILRR AVRYSHEKLN ASRGFFATLV DVVVQSLGDA 370 380 390 400 410 420 FPELKKDPDM VKDIINEEEV QFLKTLSRGR RILDRKIQSL GDCKTIPGDT AWLLYDTYGF 430 440 450 460 470 480 PVDLTGLIAE EKGLVVDMDG FEEERKLAQL KSQGKGAGGE DLIMLDIYAI EELRAKGLEA 490 500 510 520 530 540 TDDSPKYNYH SDSSGAYVFE CTVATVLALR REKMFVEEVV TGQECGVVLD KTCFYAEQGG 550 560 570 580 590 600 QIYDEGYLVK VDDSSEDKTE FTVKNAQVRG GYVLHIGTIY GNLKVGDQVR LFIDEPRRRP 610 620 630 640 650 660 VMSNHTATHI LNFALRSVLG EADQKGSLVA PDRLRFDFTA KGAMSTQQIK EAEEIVNAMI 670 680 690 700 710 720 EAAKPVYTQD CPLAAAKAIQ GLRAVFDETY PDPVRVVSIG VPVSELLDDP SGPAGSLTSV 730 740 750 760 770 780 EFCGGTHLQN SSHAGAFVIV TEEAIAKGIR RIVAVTGAEA QKALRKAESL RRSLSIMEAK 790 800 810 820 830 840 VKAQTAPNKD VQREIADLGE ALATAVIPQW QKDEQRETLK SLKKVMDDLD RASKADVQKR 850 860 870 880 890 900 VLEKTKQLID SNPNQPLVIL EMESGASAKA LNEALKLFKT HSPQTSAMLF TVDNEAGKIT 910 920 930 940 950 960 CLCQVPQNAA NRGLKASEWV QQVSGLMDGK GGGKDVSAQA TGKNVGCLQE ALQLATSFAQ LRLGAVEN

References

[1]	"A hamster temperature-sensitive alanyl-tRNA synthetase mutant causes degradation of cell-cycle related proteins and apoptosis." Wang Y., Sekiguchi T., Noguchi E., Nishimoto T. J. Biochem. 135:7-16(2004) [PubMed: 14999004] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ARG-321.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB097816 mRNA. Translation: BAC44844.1.
3D structure databases
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q8CFX8.
Family and domain databases
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProDom	Q8CFX8. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

SYAC_MESAU

Accession

Primary (citable) accession number: Q8CFX8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 3, 2006
Last sequence update:	March 1, 2003
Last modified:	July 22, 2008
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)