Reviewed, UniProtKB/Swiss-Prot Q8CNF8 (LACG_STAES)

Names and origin

Protein names

Recommended name:
    6-phospho-beta-galactosidase
    EC=3.2.1.85
Alternative name(s):
    Beta-D-phosphogalactoside galactohydrolase
      Short name=PGALase
    P-beta-Gal
      Short name=PBG

Gene names

Name:	lacG
Ordered Locus Names:	SE_1781

Organism

Staphylococcus epidermidis (strain ATCC 12228) [Complete proteome] [HAMAP]

Taxonomic identifier

176280 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	470 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	A 6-phospho-beta-D-galactoside + H(2)O = 6-phospho-D-galactose + an alcohol.
Pathway	Carbohydrate metabolism; lactose degradation; D-galactose 6-phosphate and beta-D-glucose from lactose 6-phosphate: step 1/1.
Sequence similarities	Belongs to the glycosyl hydrolase 1 family.

Ontologies

Keywords
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	galactosidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 470	470	6-phospho-beta-galactosidase
Sites
	Active site	160	1	Proton donor By similarity
	Active site	375	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8CNF8-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: E81C610AB95A77EE	FASTA	470	54,836
	10 20 30 40 50 60 MTKKLPDDFI FGGATAAYQA EGATQTDGKG RVAWDTYLEE NYWYTAEPAS DFYNRYPVDL 70 80 90 100 110 120 ELSERFGVNG IRISIAWSRI FPKGYGEVNQ KGVEYYHNLF KECHKRHVEP FVTLHHFDTP 130 140 150 160 170 180 EVLHKDGDFL NRKTIDYFVD YAEFCFKEFP EVKYWTTFNE IGPIGDGQYL VGKFPPGIKY 190 200 210 220 230 240 DFEKVFQSHH NMMVAHARAV KLFKDENYKG EIGVVHALPT KYPYDPSNPE DVRAAELEDI 250 260 270 280 290 300 IHNKFILDAT YLGKYSRETM EGVQHILSVN GGQLEISDED YKILDEAKDL NDFLGINYYM 310 320 330 340 350 360 SDWMRGFEGE SEITHNATGD KGGSKYQLKG VGQREFDVDV PRTDWDWMIY PQGLYDQIMR 370 380 390 400 410 420 VVKDYPNYHK IYITENGLGY KDVFDEKEKT VHDDARIDYI KQHLSVIADA IADGANVKGY 430 440 450 460 470 FLWSLMDVFS WSNGYEKRYG LFYVDFETQE RFPKKSAYWY KELAESKEIK

References

[1]

"Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228)." Zhang Y.-Q., Ren S.-X., Li H.-L., Wang Y.-X., Fu G., Yang J., Qin Z.-Q., Miao Y.-G., Wang W.-Y., Chen R.-S., Shen Y., Chen Z., Yuan Z.-H., Zhao G.-P., Qu D., Danchin A., Wen Y.-M. Mol. Microbiol. 49:1577-1593(2003) [PubMed: 12950922] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015929 Genomic DNA. Translation: AAO05422.1.
RefSeq	NP_765336.1.
3D structure databases
HSSP	HSSP built from PDB template 1PBG based on UniProtKB P11546.
SMR	Q8CNF8. Positions 1-470.
ModBase	Search...
Genome annotation databases
GeneID	1057113.
GenomeReviews	Gene locus SE_1781 in contig AE015929_GR.
KEGG	sep:SE1781.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8CNF8.
Enzyme and pathway databases
BioCyc	SEPI176280:SE_1781-MON.
Family and domain databases
HAMAP	MF_01574. [Tree]
InterPro	IPR001360. Glyco_hydro_1. IPR013781. Glyco_hydro_sub_cat. IPR005928. LacG. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR10353. Glyco_hydro_1. 1 hit.
Pfam	PF00232. Glyco_hydro_1. 1 hit. [Graphical view]
PRINTS	PR00131. GLHYDRLASE1.
TIGRFAMs	TIGR01233. lacG. 1 hit.
PROSITE	PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit. PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit. [Graphical view]
ProDom	Q8CNF8. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

LACG_STAES

Accession

Primary (citable) accession number: Q8CNF8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	March 1, 2003
Last modified:	September 2, 2008
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)