Reviewed, UniProtKB/Swiss-Prot Q8FT23 (SYA_COREF)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	CE1748

Organism

Corynebacterium efficiens [Complete proteome] [HAMAP]

Taxonomic identifier

152794 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	890 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 890	890	Alanyl-tRNA synthetase

Sequences

	Sequence		Length	Mass (Da)
	Q8FT23-1 [UniParc]. Last modified January 16, 2004. Version 2. Checksum: C808475D94D96DFB	FASTA	890	97,033
	10 20 30 40 50 60 MQTHEIRERF TNHFVNAGHT AVPSASLILD DPNLLFVNAG MVPFKPYFLG QQTPPFANGT 70 80 90 100 110 120 ATSIQKCVRT LDIEEVGITT RHNTFFQMAG NFSFGQYFKE GAITHAWTLL TNPVEEGGYG 130 140 150 160 170 180 LDPERLWVTV YLDDDEAAEI WEKKIGVPAE RIQRLGMADN YWSMGVPGPC GPCSEIYYDR 190 200 210 220 230 240 GPKYGNEGGP AVDDSRYLEI WNLVFMEFER GEGTGKDSFE IIGELPKKNI DTGMGVERVA 250 260 270 280 290 300 CILQGVENVY ETDLLRPVID VAETLTGATY GRDNTADIRF RVIADHSRTG MMLILDGVTP 310 320 330 340 350 360 GNEGRGYILR RLLRRIIRSA RLLGATGETM EKFMNTIMDT MTPSYPEIAE NRERILRVAI 370 380 390 400 410 420 TEERAFLKTL VSGTHLFEEA AASIKSSGST TVAGAQAFAL HDTYGFPIDL TLEMAAEAGL 430 440 450 460 470 480 EVDVEGFESL MAEQRSRAKA DSQAKKHGHA DLSIYREWVD NHPTVFTGFE ELESQSRVLG 490 500 510 520 530 540 LLSDGTRINE AAEGQEVEVI LDQSPLYAES GGQLGDRGRI LMGDTVVDIQ DVQKIGKKLW 550 560 570 580 590 600 VHKAVVANGG LAVGDEVVAA VDKQWRHAAR QAHTATHLIH AALRQVLGPT AVQAGSMNKP 610 620 630 640 650 660 GYLRFDFNYT DQLTPEQLNQ IQAITNEAVD TDWAVNTIET SLEEARAMGA MALFGENYGD 670 680 690 700 710 720 LVRVVEIGGP FSMELCGGTH VEHSSQIGPV ALLGESSIGS GVRRIEAYSG LNSFNYLSKE 730 740 750 760 770 780 RALAESLSSI LKTPSTDLPE RISQLLDKLK AAEKEIATLH RRELAAKSEE FITGAEEING 790 800 810 820 830 840 IRAVMVRVQD GLDAGDLRTL ATTLRDKLSN GEGLVVVASR SEDGTKVPFV AGATKQAVAR 850 860 870 880 890 GVHSGNLIKL IGSYIDGRGG GKPDMAQGSG SDIDGLDAAF NAVRAELENL

References

[1]

"Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens." Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T. Genome Res. 13:1572-1579(2003) [PubMed: 12840036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000035 Genomic DNA. Translation: BAC18558.1. Different initiation.
RefSeq	NP_738358.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1034248.
GenomeReviews	Gene locus CE1748 in contig BA000035_GR.
KEGG	cef:CE1748.
NMPDR	fig|196164.1.peg.1748.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8FT23.
Enzyme and pathway databases
BioCyc	CEFF196164:CE1748-MON.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProDom	Q8FT23. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

SYA_COREF

Accession

Primary (citable) accession number: Q8FT23

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	January 16, 2004
Last modified:	July 22, 2008
This is version 34 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)