Reviewed, UniProtKB/Swiss-Prot Q8H9A8 (COOMT_COPJA)

Names and origin

Protein names	Recommended name:     Columbamine O-methyltransferase       Short name=CoOMT     EC=2.1.1.118
Organism	Coptis japonica (Japanese goldthread)
Taxonomic identifier	3442 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › Ranunculales › Ranunculaceae › Coptis

Protein attributes

Sequence length	351 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the conversion of columbamine to palmatine, an isoquinoline alkaloid.
Catalytic activity	S-adenosyl-L-methionine + columbamine = S-adenosyl-L-homocysteine + palmatine.
Pathway	Alkaloid biosynthesis; palmatine biosynthesis; palmatine from columbamine: step 1/1.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. Type 2 family. COMT subfamily.
Biophysicochemical properties	Kinetic parameters: KM=66 µM for columbamine KM=35 µM for tetrahydrocolumbine KM=173 µM for (S)-scoulerine

Ontologies

Keywords
Biological process	Alkaloid metabolism
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	alkaloid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	columbamine O-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 351	351	Columbamine O-methyltransferase		PRO_0000204432
Sites
	Active site	259	1	Proton acceptor By similarity
	Binding site	198	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	221	1	S-adenosyl-L-methionine By similarity
	Binding site	241	1	S-adenosyl-L-methionine By similarity
	Binding site	242	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	255	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8H9A8-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: B0D2B88F477F9A28	FASTA	351	39,282
	10 20 30 40 50 60 MDTPNTFQND DEIKAQAQVW KHMFGFAETI MLRSTVSLGI PDIIHNNGPV TLSQLVTHLP 70 80 90 100 110 120 LKSTSIDRFH HFMRYLVHMQ LFTISTDQIT KEDKYELTPA SKLLVHGHQK SLAPYVMLQT 130 140 150 160 170 180 HPEEFSVWSH VINVLDGKKP YWESNDTSMY EKTEGDPEIN EILNDAMTSH STFMLPALVS 190 200 210 220 230 240 GLMKENVLDG VASIVDVGGN SGVVAKGIVD AFPHVKCSVM DLNHVIERVI KNPKLDYVAG 250 260 270 280 290 300 DMFTSIPNAD AILLKSTLHN YEDDDCIKIL NIAKEALPST GGKVILVEIV VDTENLPLFT 310 320 330 340 350 SARLSMGMDM MLMSGKERTK KEWEDLLRKA NFTSHQVIPI MAIESIIVAY S

References

[1]

"Molecular cloning of columbamine O-methyltransferase from cultured Coptis japonica cells." Morishige T., Dubouzet E., Choi K.-B., Yazaki K., Sato F. Eur. J. Biochem. 269:5659-5667(2002) [PubMed: 12423366] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ENZYME ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB073908 mRNA. Translation: BAC22084.1.
3D structure databases
HSSP	HSSP built from PDB template 1FP2 based on UniProtKB O24529.
ModBase	Search...
Family and domain databases
InterPro	IPR016461. O-MeTrfase_COMT_euk. IPR001077. O_MeTrfase_2. IPR012967. Plant_MeTrfase_dimerisation. IPR011991. Wing_hlx_DNA_bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
Pfam	PF08100. Dimerisation. 1 hit. PF00891. Methyltransf_2. 1 hit. [Graphical view]
PIRSF	PIRSF005739. O-mtase. 1 hit.
BLOCKS	Search...
ProtoNet	Search...

Entry information

Entry name

COOMT_COPJA

Accession

Primary (citable) accession number: Q8H9A8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	March 1, 2003
Last modified:	November 4, 2008
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)