Reviewed, UniProtKB/Swiss-Prot Q8IVW6 (ARI3B_HUMAN)

Names and origin

Protein names

Recommended name:
    AT-rich interactive domain-containing protein 3B
Alternative name(s):
    ARID domain-containing protein 3B
    Bright-like
    Bright and dead ringer protein

Gene names

Name:	ARID3B
Synonyms:	BDP, DRIL2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	561 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Transcription factor which may be involved in neuroblastoma growth and malignant transformation. Favors nuclear targeting of ARID3A.
Subunit structure	Heterodimer with ARID3A. Interacts with unphosphorylated RB1.
Subcellular location	Nucleus.
Tissue specificity	Expressed in placenta, testis and leukocytes. Expressed in neuroblastoma. Present in K562 erythrocytic leukemia cells (at protein level).
Sequence similarities	Contains 1 ARID domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	DNA-binding
Molecular function	Oncogene
Gene Ontology (GO)
Cellular component	nucleus Ref.1 Non-traceable author statement. Source: UniProtKB
Molecular function	DNA binding Ref.1 Non-traceable author statement. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8IVW6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8IVW6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     234-253: TPINRIPIMAKQILDLYMLY → ECASTHHSNSGNTDRVPTVC
     254-561: Missing.

Notes: No experimental confirmation available.

Isoform 3 (identifier: Q8IVW6-3)

The sequence of this isoform differs from the canonical sequence as follows:
     390-471: DGAPVTTVPV...RQLPMKIRIN → QQGSWGRRKK...PVPLQVMEPQ
     472-561: Missing.

Notes: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 561	561	AT-rich interactive domain-containing protein 3B
Regions
	Domain	215 – 307	93	ARID
	Region	203 – 365	163	Interaction with RB1
	Region	490 – 513	24	Interaction with ARID3A
	Compositional bias	5 – 49	45	Gln-rich
	Compositional bias	341 – 353	13	Poly-Ala
	Compositional bias	523 – 560	38	Ser-rich
Natural variations
	Alternative sequence	234 – 253	20	TPINR…LYMLY → ECASTHHSNSGNTDRVPTVC in isoform 2.
	Alternative sequence	254 – 561	308	Missing in isoform 2.
	Alternative sequence	390 – 471	82	DGAPV…KIRIN → QQGSWGRRKKGRKGCQFPVF KTGVLGQEGQLWIGPAPVPL QVMEPQ in isoform 3.
	Alternative sequence	472 – 561	90	Missing in isoform 3.
Experimental info
	Mutagenesis	240	1	P → H: Impairs binding to RB1
	Mutagenesis	271	1	W → S: Impairs binding to RB1
	Sequence conflict	7	1	Q → QQ in AAH41792. Ref.3
	Sequence conflict	474	1	Missing in AAD09133. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 10, 2007. Version 2. Checksum: 5F0CFF83BAFC12D2	FASTA	561	60,637
	10 20 30 40 50 60 MEPLQQQQQQ QQQQQKQPHL APLQMDAREK QGQQMREAQF LYAQKLVTQP TLLSATAGRP 70 80 90 100 110 120 SGSTPLGPLA RVPPTAAVAQ VFERGNMNSE PEEEDGGLED EDGDDEVAEV AEKETQAASK 130 140 150 160 170 180 YFHVQKVARQ DPRVAPMSNL LPAPGLPPHG QQAKEDHTKD ASKASPSVST AGQPNWNLDE 190 200 210 220 230 240 QLKQNGGLAW SDDADGGRGR EISRDFAKLY ELDGDPERKE FLDDLFVFMQ KRGTPINRIP 250 260 270 280 290 300 IMAKQILDLY MLYKLVTEKG GLVEIINKKI WREITKGLNL PTSITSAAFT LRTQYMKYLY 310 320 330 340 350 360 AYECEKKALS SPAELQAAID GNRREGRRPS YSSSLFGYSP AAATAAAAAG APALLSPPKI 370 380 390 400 410 420 RFPILGLGSS SGTNTSSPRI SPATTLRKGD GAPVTTVPVP NRLAVPVTLA SQQAGTRTAA 430 440 450 460 470 480 LEQLRERLES GEPAEKKASR LSEEEQRLVQ QAFQRNFFSM ARQLPMKIRI NGRAEDRAEA 490 500 510 520 530 540 SAAALNLTTS SIGSINMSVD IDGTTYAGVL FAQKPVVHLI TGSAPQSLGS SASSSSSSHC 550 560 SPSPTSSRGT PSAEPSTSWS L
	Isoform 2 [UniParc]. Checksum: CCD27200866E8B03		253	27,837
	10 20 30 40 50 60 MEPLQQQQQQ QQQQQKQPHL APLQMDAREK QGQQMREAQF LYAQKLVTQP TLLSATAGRP 70 80 90 100 110 120 SGSTPLGPLA RVPPTAAVAQ VFERGNMNSE PEEEDGGLED EDGDDEVAEV AEKETQAASK 130 140 150 160 170 180 YFHVQKVARQ DPRVAPMSNL LPAPGLPPHG QQAKEDHTKD ASKASPSVST AGQPNWNLDE 190 200 210 220 230 240 QLKQNGGLAW SDDADGGRGR EISRDFAKLY ELDGDPERKE FLDDLFVFMQ KRGECASTHH 250 SNSGNTDRVP TVC
	Isoform 3 [UniParc]. Checksum: 10364C23137AFDB9		435	47,661
	10 20 30 40 50 60 MEPLQQQQQQ QQQQQKQPHL APLQMDAREK QGQQMREAQF LYAQKLVTQP TLLSATAGRP 70 80 90 100 110 120 SGSTPLGPLA RVPPTAAVAQ VFERGNMNSE PEEEDGGLED EDGDDEVAEV AEKETQAASK 130 140 150 160 170 180 YFHVQKVARQ DPRVAPMSNL LPAPGLPPHG QQAKEDHTKD ASKASPSVST AGQPNWNLDE 190 200 210 220 230 240 QLKQNGGLAW SDDADGGRGR EISRDFAKLY ELDGDPERKE FLDDLFVFMQ KRGTPINRIP 250 260 270 280 290 300 IMAKQILDLY MLYKLVTEKG GLVEIINKKI WREITKGLNL PTSITSAAFT LRTQYMKYLY 310 320 330 340 350 360 AYECEKKALS SPAELQAAID GNRREGRRPS YSSSLFGYSP AAATAAAAAG APALLSPPKI 370 380 390 400 410 420 RFPILGLGSS SGTNTSSPRI SPATTLRKGQ QGSWGRRKKG RKGCQFPVFK TGVLGQEGQL 430 WIGPAPVPLQ VMEPQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Bdp, a new member of a family of DNA-binding proteins, associates with the retinoblastoma gene product." Numata S., Claudio P.P., Dean C., Giordano A., Croce C.M. Cancer Res. 59:3741-3747(1999) [PubMed: 10446990] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, SUBCELLULAR LOCATION, DNA-BINDING, INTERACTION WITH RB1, MUTAGENESIS OF PRO-240 AND TRP-271. Tissue: Testis.
[2]	Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S., Ohara O., Nagase T., Kikuno R.F. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Brain.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Placenta and Testis.
[4]	"ARID3B induces malignant transformation of mouse embryonic fibroblasts and is strongly associated with malignant neuroblastoma." Kobayashi K., Era T., Takebe A., Jakt L.M., Nishikawa S. Cancer Res. 66:8331-8336(2006) [PubMed: 16951138] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
[5]	"REKLES is an ARID3-restricted multifunctional domain." Kim D., Probst L., Das C., Tucker P.W. J. Biol. Chem. 282:15768-15777(2007) [PubMed: 17400556] [Abstract] Cited for: INTERACTION WITH ARID3A, SUBCELLULAR LOCATION, FUNCTION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF116846 mRNA. Translation: AAD09133.1. AB208830 mRNA. Translation: BAD92067.1. Different initiation. BC041792 mRNA. Translation: AAH41792.1. BC060824 mRNA. Translation: AAH60824.1.
RefSeq	NP_006456.1.
UniGene	Hs.655532
3D structure databases
HSSP	HSSP built from PDB template 1KQQ based on UniProtKB Q24573.
SMR	Q8IVW6. Positions 197-327.
ModBase	Search...
PTM databases
PhosphoSite	Q8IVW6.
Genome annotation databases
Ensembl	ENSG00000179361. Homo sapiens. [Contig view]
GeneID	10620.
KEGG	hsa:10620.
Organism-specific databases
HGNC	HGNC:14350. ARID3B.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	Q8IVW6.
HOVERGEN	Q8IVW6.
Gene expression databases
ArrayExpress	Q8IVW6.
CleanEx	HS_ARID3B.
Family and domain databases
InterPro	IPR001606. ARID. [Graphical view]
Gene3D	G3DSA:1.10.150.60. ARID. 1 hit.
Pfam	PF01388. ARID. 1 hit. [Graphical view]
SMART	SM00501. BRIGHT. 1 hit. [Graphical view]
PROSITE	PS51011. ARID. 1 hit. [Graphical view]
ProDom	Q8IVW6. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

ARI3B_HUMAN

Accession

Primary (citable) accession number: Q8IVW6
Secondary accession number(s): O95443, Q59HC9, Q6P9C9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	July 10, 2007
Last modified:	July 22, 2008
This is version 32 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)