Reviewed, UniProtKB/Swiss-Prot Q8K9E7 (SYA_BUCAP)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	BUsg_390

Organism

Buchnera aphidicola subsp. Schizaphis graminum [Complete proteome] [HAMAP]

Taxonomic identifier

98794 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	883 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 883	883	Alanyl-tRNA synthetase

Sequences

	Sequence		Length	Mass (Da)
	Q8K9E7-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 7D1DB0D46F640AF8	FASTA	883	102,345
	10 20 30 40 50 60 MKKTTNEIRQ SFLNFFKEKE HVIVPSSSLI PENDSTLLFT NAGMNQFKEY FLGQKKKFYP 70 80 90 100 110 120 RVTTVQNCLR TGGKHNDLEN VGYTKRHHTF FEMLGNFSFN DYFKKEAITY AWELLTSRKW 130 140 150 160 170 180 FNIDKNKLWI SVYEDDEETY KIWRDIIRIP CHHIVKIGSK NNSQYDSENF WQMGETGPCG 190 200 210 220 230 240 PCTEIFYNYD DSNKSNDFLK DKNESFIEIW NIVFIEFNRI SKTKIVPLIN KSIDTGMGLE 250 260 270 280 290 300 RISAVLQNVH SNYKIDIFQK LIQKISNFTE IKDLNNISLK IIADHIRSCA FLIAENILPS 310 320 330 340 350 360 NEHRGYVLRR IIRRALRHGH KIGIKNNFFY KLVPSLIEIM GDSAKILRKK EKIIEETLKI 370 380 390 400 410 420 EEIQFSQTLD KGLKILNAEI KKSTNKTISG KTAFYLYDTF GFPIDLTSDI CSEKNIKIDF 430 440 450 460 470 480 KGFNIAKEEQ KKRSSIKNKF YKDYNKDIII NDTCIFEGYK KNKTKSLVKY IFIKNESVFL 490 500 510 520 530 540 IYKGQTATIF LDKTSFYPES GGQIGDIGEL YHKKSRFIVE NTKKYGDTIG HYGKLISGKI 550 560 570 580 590 600 IVNDSIYSKI NHVYRNAIQL NHSATHLLHA ALQKVLGKNA IQKGSLVSNT HLRFDFSYSG 610 620 630 640 650 660 NINLSQIQNI ENIINKKIRS NDLIKIKNLS LEEAKKKKAI ALFDYKYQSS VRVVFIKDFS 670 680 690 700 710 720 IELCGGTHTK RTGNIGLFKI IEQSSVSSGI KRIEAVTGQQ AIDYLHIKDN DMQNISFLLK 730 740 750 760 770 780 CQNSKITEKI KKIIIQVEKL EKKTDQLQKR ENIYQIKKLS KKINNIKGIN LLINTFTNYD 790 800 810 820 830 840 QKSMKMIIDQ LKKELKISII IFINKNKNDF TVIIRVTKNL INYITALKII NIFIKKANGK 850 860 870 880 GGGKKEIAEG GGMNIKKLPM ILNYIKSWIT IQLENIKTKN FNN

References

[1]

"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE013218 Genomic DNA. Translation: AAM67942.1.
RefSeq	NP_660731.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1005767.
GenomeReviews	Gene locus BUsg_390 in contig AE013218_GR.
KEGG	bas:BUsg390.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8K9E7.
Enzyme and pathway databases
BioCyc	BAPH198804:BUSG390-MON.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProDom	Q8K9E7. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

SYA_BUCAP

Accession

Primary (citable) accession number: Q8K9E7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	October 1, 2002
Last modified:	July 22, 2008
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)