Reviewed, UniProtKB/Swiss-Prot Q8KA60 (ASSY_BUCAP)

Names and origin

Protein names

Recommended name:
    Argininosuccinate synthase
    EC=6.3.4.5
Alternative name(s):
    Citrulline--aspartate ligase

Gene names

Name:	argG
Ordered Locus Names:	BUsg_047

Organism

Buchnera aphidicola subsp. Schizaphis graminum [Complete proteome] [HAMAP]

Taxonomic identifier

98794 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	401 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3.
Subunit structure	Homotetramer By similarity.
Subcellular location	CytoplasmProbable.
Sequence similarities	Belongs to the argininosuccinate synthase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP argininosuccinate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 401	401	Argininosuccinate synthase
Regions
	Nucleotide binding	10 – 18	9	ATP By similarity
Sites
	Binding site	37	1	ATP; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	89	1	Citrulline By similarity
	Binding site	119	1	ATP; via amide nitrogen By similarity
	Binding site	121	1	Aspartate By similarity
	Binding site	125	1	Aspartate By similarity
	Binding site	125	1	Citrulline By similarity
	Binding site	126	1	Aspartate By similarity
	Binding site	129	1	Citrulline By similarity
	Binding site	178	1	Citrulline By similarity
	Binding site	187	1	Citrulline By similarity
	Binding site	263	1	Citrulline By similarity
	Binding site	275	1	Citrulline By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8KA60-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 16DD4B6F98E13D8C	FASTA	401	45,124
	10 20 30 40 50 60 MNNFKKVVLA YSGGLDTSAI IPWLKENYHV DVVAFVADIG QSKKDLNGIE KKALQSGAID 70 80 90 100 110 120 CRVFDLKEEF IKDYVYPVLK TGSLYEGNYL LGTAIARPII AKKQVEFASS IGAKFLCHGA 130 140 150 160 170 180 TGKGNDQVRF EIAYAALAPY MKVIAPWREW NLNSRESLLE YLREKNIPTS ATLEKIYSRD 190 200 210 220 230 240 ENSLHISTEG GLLEDLWNKP NADCWNWTVE LEDAPEQPEY ISLIVKNGSV VSVNNEFLTP 250 260 270 280 290 300 LKCIEKLNKI GSKHAIGRID IVENRLVGIK SRGCYETPGG TIMHIALRAI EQLVFDRESF 310 320 330 340 350 360 KWREKIGLEM SSVVYDGRWF TPIRKSLQAS ADSLSSEVNG EVVLKLYKGS VIPVQKKSSN 370 380 390 400 SLYSKEYATF GEDEVYKHSD AEGFIRLFSL SSRIRAQNKL K

References

[1]

"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE013218 Genomic DNA. Translation: AAM67618.1.
RefSeq	NP_660407.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1005863.
GenomeReviews	Gene locus BUsg_047 in contig AE013218_GR.
KEGG	bas:BUsg047.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8KA60.
Enzyme and pathway databases
BioCyc	BAPH198804:BUSG047-MON.
Family and domain databases
HAMAP	MF_00005. [Tree]
InterPro	IPR001518. Arginosuc_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11587. Arginosuc_synth. 1 hit.
Pfam	PF00764. Arginosuc_synth. 1 hit. [Graphical view]
TIGRFAMs	TIGR00032. argG. 1 hit.
PROSITE	PS00564. ARGININOSUCCIN_SYN_1. 1 hit. PS00565. ARGININOSUCCIN_SYN_2. 1 hit. [Graphical view]
ProDom	Q8KA60. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

ASSY_BUCAP

Accession

Primary (citable) accession number: Q8KA60

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	October 1, 2002
Last modified:	July 22, 2008
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)