Reviewed, UniProtKB/Swiss-Prot Q8KA63 (MURB_BUCAP)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylenolpyruvoylglucosamine reductase
    EC=1.1.1.158
Alternative name(s):
    UDP-N-acetylmuramate dehydrogenase

Gene names

Name:	murB
Ordered Locus Names:	BUsg_042

Organism

Buchnera aphidicola subsp. Schizaphis graminum [Complete proteome] [HAMAP]

Taxonomic identifier

98794 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	UDP-N-acetylmuramate + NADP(+) = UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH.
Cofactor	FAD By similarity.
Pathway	Cell wall biosynthesis; peptidoglycan biosynthesis.
Subcellular location	CytoplasmProbable.
Sequence similarities	Belongs to the murB family. Contains 1 FAD-binding PCMH-type domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	UDP-N-acetylmuramate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 344	344	UDP-N-acetylenolpyruvoylglucosamine reductase
Regions
	Domain	19 – 189	171	FAD-binding PCMH-type
Sites
	Active site	165	1	By similarity
	Active site	235	1	Proton donor By similarity
	Active site	331	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8KA63-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 4D3E909931F725CB	FASTA	344	39,519
	10 20 30 40 50 60 MYKKKKSYQS LKNLNTFSIN VTAKKIIFVK TIQSLMKIWK TCNLSNIPYI ILGEGSNVLF 70 80 90 100 110 120 LENYAGVVII NRIKGIRIEE KKKNWLLHVF SGEKWHDLVK YTLRMGFFGL ENLALIPGSV 130 140 150 160 170 180 GSAAIQNIGA YGLELKNICQ YVDVISLENG KTIRLKKKTC NFSYRSSIFK YKYNNGYAVI 190 200 210 220 230 240 AVGIKIKKNW KPVIFSSLLK SKKILEINAY KIFNIVCQIR KKKLPNLKKL GNAGSFFKNP 250 260 270 280 290 300 IITSKKTKKI LSSYMKMPYY IQKNGFIKIP AAWLIEKYNF KNIQIGDAAI YKKQKLILIN 310 320 330 340 LKKANSKDIL KLAQIIQKCI LKKFGIYLEP EVDFINSLGK VKLL

References

[1]

"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE013218 Genomic DNA. Translation: AAM67613.1.
RefSeq	NP_660402.1.
3D structure databases
HSSP	HSSP built from PDB template 2MBR based on UniProtKB P08373.
ModBase	Search...
Genome annotation databases
GeneID	1005858.
GenomeReviews	Gene locus BUsg_042 in contig AE013218_GR.
KEGG	bas:BUsg042.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8KA63.
Enzyme and pathway databases
BioCyc	BAPH198804:BUSG042-MON.
Family and domain databases
HAMAP	MF_00037. [Tree]
InterPro	IPR016169. CO_DHase_flavot_FAD-bd_sub2. IPR016167. FAD-bd_2_sub1. IPR003170. MurB. IPR011601. MurB_C. IPR006094. Oxid_FAD_bind_N. [Graphical view]
Gene3D	G3DSA:3.30.465.10. CO_DH_flavoprot_FAD-bd_sub2. 1 hit. G3DSA:3.30.43.10. FAD-binding_2_sub1. 1 hit. G3DSA:3.90.78.10. MurB_C. 1 hit.
PANTHER	PTHR21071. MurB. 1 hit.
Pfam	PF01565. FAD_binding_4. 1 hit. PF02873. MurB_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00179. murB. 1 hit.
PROSITE	PS51387. FAD_PCMH. 1 hit. [Graphical view]
ProDom	Q8KA63. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

MURB_BUCAP

Accession

Primary (citable) accession number: Q8KA63

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	October 1, 2002
Last modified:	July 22, 2008
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)