Reviewed, UniProtKB/Swiss-Prot Q8KA71 (METE_BUCAP)

Names and origin

Protein names

Recommended name:
    5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
    EC=2.1.1.14
Alternative name(s):
    Methionine synthase, vitamin-B12 independent isozyme
    Cobalamin-independent methionine synthase

Gene names

Name:	metE
Ordered Locus Names:	BUsg_031

Organism

Buchnera aphidicola subsp. Schizaphis graminum [Complete proteome] [HAMAP]

Taxonomic identifier

98794 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	757 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity.
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1.
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 757	757	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
Sites
	Metal binding	640	1	Zinc By similarity
	Metal binding	642	1	Zinc By similarity
	Metal binding	725	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8KA71-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 637B1D9532325BCE	FASTA	757	88,098
	10 20 30 40 50 60 MTIFNHTLGF PRIGLNRELK KAQEDYWSGN LLYEDFLCVG SQLRRENWKK QKDAGIDYIP 70 80 90 100 110 120 VGDFAWYDHV LSTSMMLGNI PERHLNVNDN KIDLDCLFRI ARGCSPDVAA SEMTKWFNTN 130 140 150 160 170 180 YHYIVPEFNK NRILKFSWKQ ILEETDEALL LGYKVKPIIL GPITYLWLGK IKGEYFDRLD 190 200 210 220 230 240 LLKNILPIYK QVLRELSQRN IDFVQIDEPA LVLDLPEKWK KAYSYAYEYL NGTTKILLTT 250 260 270 280 290 300 YFDSIEHNIE YIRDLPIYGI HIDLVFGKYN LFDFNSKLPK EWILSLGVIN GRNVWRADLI 310 320 330 340 350 360 KLFKSISTIL KFRKKLLIGS SCSLLHSPID LQKEKNLDKE SKKWFSFAVQ KCTELSLLSK 370 380 390 400 410 420 ALNKNDTDSI QKWCSSIYER NFSKRVQKIE VQNRLSEILN VNFKRSDSYN IRSREQKKKF 430 440 450 460 470 480 NLPILPTTTI GSFPQTLQIR KLRRDYKQGL INDVDYEIGI KKHIKEVIKE QEKLGIDVLV 490 500 510 520 530 540 HGEPERNDMV EYFGENLDGF IFTDNGWVQS YGSRCVKPPI IIGDVSRLKP ITVKWSKYAQ 550 560 570 580 590 600 SLTDKPVKAM LTGPVTILFW SFPREDISLE KIAKQIGLAL RDEVLDLEKE KIEIIQIDEP 610 620 630 640 650 660 ALREGLPLRK SLWNEYLSWA VDAFRLSSSG VKNTTQIHTH MCYCEFHDIM HAISFLDADV 670 680 690 700 710 720 ITIETARSDM ELLKSFKTFK YPNEIGPGVY DIHSSNIPSI TSIKFLLNKA IAYIPVERIW 730 740 750 VNPDCGLKTR NWNETILALK NMVCAAKEMR DKIKKIT

References

[1]

"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE013218 Genomic DNA. Translation: AAM67602.1.
RefSeq	NP_660391.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1005847.
GenomeReviews	Gene locus BUsg_031 in contig AE013218_GR.
KEGG	bas:BUsg031.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8KA71.
Enzyme and pathway databases
BioCyc	BAPH198804:BUSG031-MON.
Family and domain databases
HAMAP	MF_00172. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR002629. Methionine_synth. IPR006276. MeTrfase_B12_ind. [Graphical view]
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
ProDom	PD004692. Methionine_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01371. met_syn_B12ind. 1 hit.
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

METE_BUCAP

Accession

Primary (citable) accession number: Q8KA71

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	October 1, 2002
Last modified:	July 22, 2008
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)