Reviewed, UniProtKB/Swiss-Prot Q8KG04 (SYA_CHLTE)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	CT0166

Organism

Chlorobium tepidum [Complete proteome] [HAMAP]

Taxonomic identifier

1097 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobaculum

Protein attributes

Sequence length	885 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 885	885	Alanyl-tRNA synthetase

Sequences

	Sequence		Length	Mass (Da)
	Q8KG04-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: BD8C19516FC0A1A7	FASTA	885	97,181
	10 20 30 40 50 60 MNSREIRQSF LDFFAGKEHR IVRSAPVIPA EDPTLLFTNA GMNQFKDVFL GKGTREYTRA 70 80 90 100 110 120 ADTQKCIRAS GKHNDLEDVG RDTYHHTFFE MLGNWSFGDY YKKEAIGWAW ELMTEVWKLP 130 140 150 160 170 180 KERLYATVYH DDEESFKLWQ SETDIEHSHI LKFGDKDNFW EMGETGPCGP CSEIHIDLTP 190 200 210 220 230 240 DGSGGPLVNV GDHRVIELWN LVFIQYDRQA DGTLVPLPQK HVDTGMGFER VAAVLQGKSS 250 260 270 280 290 300 NYDSDVFAPL FDRITELTGT VYTASLDSPS DIAMRVIADH CRTLTFALSD GAMPGNEGRG 310 320 330 340 350 360 YVLRRILRRA VRYAGTLGCH EPIIYKMVEV LVRTMGDVFP ELEKQQATVE KIIRAEEESF 370 380 390 400 410 420 LVTLGRGTEI FNEVVADMKT AGSTTISGAD AFKLYDTFGF PLDLTRLMAA EVGLGIDEAG 430 440 450 460 470 480 FEHCMMEQKT RARMDRKGKM QMQDDGGEWQ WFAPEAPTTF VGYDMLETEA TLVAARVSGD 490 500 510 520 530 540 KLLMVLDQTP FYAESGGQVG DHGTLETAGY RLDVTDTRKD GEQIVHVVTS AHDKVRDCAV 550 560 570 580 590 600 TPADVSFDGV VSVKAAVDRD RRVATERNHT ATHLLHAALR KVLGEHVQQK GSLVTPERLR 610 620 630 640 650 660 FDFSHFSKVS AEELEQVEHE VNAEIRKAAS VTKHADVPYE EALALGALAF FGDKYADRVR 670 680 690 700 710 720 VVEVPGISIE LCGGTHVGNI GQIGMVKIVS ESSVAAGIRR IEAVTGAAAE ALLWQEYRDL 730 740 750 760 770 780 QEIKNLLKLK ADEEAGPKIK ELLEEKKALD KQLQESRLAG LLDRLAASLA GGEEVNGCRI 790 800 810 820 830 840 MTERLDGVSG DELRQAAVAL RERVPCAVGL LCGVADGKVS LVAFASDEAV KSLKLDAGKL 850 860 870 880 VKEAAASVKG GGGGKLELAT AGGKEPAGID KAIEVFVASV KSALQ

References

[1]

"The complete genome sequence of Chlorobium tepidum TLS, a photosynthetic, anaerobic, green-sulfur bacterium." Eisen J.A., Nelson K.E., Paulsen I.T., Heidelberg J.F., Wu M., Dodson R.J., DeBoy R.T., Gwinn M.L., Nelson W.C., Haft D.H., Hickey E.K., Peterson J.D., Durkin A.S., Kolonay J.F., Yang F., Holt I.E., Umayam L.A., Mason T.M. , Brenner M., Shea T.P., Parksey D.S., Nierman W.C., Feldblyum T.V., Hansen C.L., Craven M.B., Radune D., Vamathevan J.J., Khouri H.M., White O., Gruber T.M., Ketchum K.A., Venter J.C., Tettelin H., Bryant D.A., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:9509-9514(2002) [PubMed: 12093901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49652 / DSM 12025 / TLS.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE006470 Genomic DNA. Translation: AAM71414.1.
RefSeq	NP_661072.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1007188.
GenomeReviews	Gene locus CT0166 in contig AE006470_GR.
KEGG	cte:CT0166.
NMPDR	fig|194439.1.peg.166.
TIGR	CT0166.
Phylogenomic databases
HOGENOM	Q8KG04.
Enzyme and pathway databases
BioCyc	CTEP194439:CT_0166-MON.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProDom	Q8KG04. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

SYA_CHLTE

Accession

Primary (citable) accession number: Q8KG04

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	October 1, 2002
Last modified:	July 22, 2008
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)