Reviewed, UniProtKB/Swiss-Prot Q8LB02 (DHSB2_ARATH)

Names and origin

Protein names

Recommended name:
    Succinate dehydrogenase [ubiquinone] iron-sulfur subunit 2, mitochondrial
    EC=1.3.5.1
Alternative name(s):
    Iron-sulfur subunit of complex II
      Short name=Ip

Gene names

Name:	SDH2-2
Ordered Locus Names:	At5g40650
ORF Names:	MNF13.21, MNF13.170

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	280 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) By similarity.
Catalytic activity	Succinate + ubiquinone = fumarate + ubiquinol.
Cofactor	Binds 1 2Fe-2S cluster By similarity. Binds 1 3Fe-4S cluster By similarity. Binds 1 4Fe-4S cluster By similarity.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle.
Subunit structure	Component of complex II composed of four subunits: the flavoprotein (FP) SDHA, iron-sulfur protein (IP) SDHB, and a cytochrome b composed of a large and a small subunit By similarity.
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein; Matrix side.
Tissue specificity	Ubiquitous. Preferentially expressed in flowers, inflorescences and root tips.
Developmental stage	Expressed in floral meristems and sex organ primordia at early stages of development. Later expressed in anthers, particularly in the tapetum, pollen mother cells, and microspores.
Sequence similarities	Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family. Contains 1 2Fe-2S ferredoxin-type domain. Contains 1 4Fe-4S ferredoxin-type domain.

Ontologies

Keywords
Biological process	Electron transport Transport Tricarboxylic acid cycle
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Ligand	2Fe-2S 3Fe-4S 4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Transit peptide	1 – 28	28	Mitochondrion Potential
	Chain	29 – 280	252	Succinate dehydrogenase [ubiquinone] iron-sulfur subunit 2, mitochondrial
Regions
	Domain	51 – 140	90	2Fe-2S ferredoxin-type
	Domain	183 – 213	31	4Fe-4S ferredoxin-type
Sites
	Metal binding	101	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	106	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	109	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	121	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	193	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	196	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	199	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	203	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	250	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	256	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	260	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Binding site	208	1	Ubiquinone; shared with DHSD By similarity
Experimental info
	Sequence conflict	14	1	S → P Ref.1 Ref.4
	Sequence conflict	31	1	T → K Ref.1 Ref.4
	Sequence conflict	127	1	S → F in CAC19856. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q8LB02-1 [UniParc]. Last modified July 25, 2006. Version 2. Checksum: 1F505A8C7D68DACD	FASTA	280	31,141
	10 20 30 40 50 60 MAFGLIGRVV GTKSSRLSTA ARLIPARWTS TGSEAQSKAS TGGGGASLKT FQIYRWNPDN 70 80 90 100 110 120 PGKPELQDYK IDLKDCGPMV LDALIKIKNE MDPSLTFRRS CREGICGSCA MNIDGCNGLA 130 140 150 160 170 180 CLTKIESGSK ETTITPLPHM FVIKDLVVDM TNFYNQYKSI EPWLKRKNPA SVPGKEILQS 190 200 210 220 230 240 KKDRAKLDGM YECILCACCS TSCPSYWWNP ESYLGPAALL HANRWISDSR DEYTKERLEA 250 260 270 280 IDDEFKLYRC HTILNCARAC PKGLNPGKQI THIKQLQKSG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Three different genes encode the iron-sulphur subunit of succinate dehydrogenase in Arabidopsis thaliana." Figueroa P., Leon G., Elorza A., Holuigue L., Jordana X. Plant Mol. Biol. 46:241-250(2001) [PubMed: 11442063] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Strain: cv. Columbia.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence features of the regions of 1,456,315 bp covered by nineteen physically assigned P1 and TAC clones." Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N., Tabata S. DNA Res. 5:41-54(1998) [PubMed: 9628582] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"The four subunits of mitochondrial respiratory complex II are encoded by multiple nuclear genes and targeted to mitochondria in Arabidopsis thaliana." Figueroa P., Leon G., Elorza A., Holuigue L., Araya A., Jordana X. Plant Mol. Biol. 50:725-734(2002) [PubMed: 12374303] [Abstract] Cited for: SUBUNIT, TISSUE SPECIFICITY.
[6]	"Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins." Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H. Plant Cell 16:241-256(2004) [PubMed: 14671022] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Nuclear SDH2-1 and SDH2-2 genes, encoding the iron-sulfur subunit of mitochondrial complex II in Arabidopsis, have distinct cell-specific expression patterns and promoter activities." Elorza A., Leon G., Gomez I., Mouras A., Holuigue L., Araya A., Jordana X. Plant Physiol. 136:4072-4087(2004) [PubMed: 15563621] [Abstract] Cited for: DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ278911 mRNA. Translation: CAC19856.1. AB009052 Genomic DNA. Translation: BAB08537.1. AY074299 mRNA. Translation: AAL66996.1. AY123036 mRNA. Translation: AAM67569.1. AY087504 mRNA. Translation: AAM65047.1.
RefSeq	NP_198881.1.
UniGene	At.20145
3D structure databases
HSSP	HSSP built from PDB template 1NEK based on UniProtKB P07014.
ModBase	Search...
Genome annotation databases
GeneID	834065.
GenomeReviews	Gene locus AT5G40650 in contig BA000015_GR.
KEGG	ath:AT5G40650.
NMPDR	fig|3702.1.peg.25769.
Organism-specific databases
GeneFarm	2169. 175.
TAIR	At5g40650.
Gene expression databases
GermOnline	AT5G40650. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR006058. 2Fe2S_fd_BS. IPR001450. 4Fe4S_Fe_S_bd. IPR012675. b-grasp_ferredoxin-like. IPR001041. Ferredoxin. IPR012285. Fum_reductase_C. IPR004489. Succ_DHase/fum_Rdtase_Fe-S. [Graphical view]
Gene3D	G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit. G3DSA:1.10.1060.10. Fum_reductase_C. 1 hit.
Pfam	PF00111. Fer2. 1 hit. [Graphical view]
TIGRFAMs	TIGR00384. dhsB. 1 hit.
PROSITE	PS00197. 2FE2S_FER_1. 1 hit. PS51085. 2FE2S_FER_2. 1 hit. PS00198. 4FE4S_FER_1. 1 hit. PS51379. 4FE4S_FER_2. 1 hit. [Graphical view]
ProDom	Q8LB02. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

DHSB2_ARATH

Accession

Primary (citable) accession number: Q8LB02
Secondary accession number(s): Q9FM32, Q9G3L9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	July 25, 2006
Last modified:	September 2, 2008
This is version 47 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)