Reviewed, UniProtKB/Swiss-Prot Q8LBZ7 (DHSB1_ARATH)

Names and origin

Protein names

Recommended name:
    Succinate dehydrogenase [ubiquinone] iron-sulfur subunit 1, mitochondrial
    EC=1.3.5.1
Alternative name(s):
    Iron-sulfur subunit of complex II
      Short name(s)=Ip

Gene names

Name:	SDH2-1
Ordered Locus Names:	At3g27380
ORF Names:	K1G2.9

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	279 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) By similarity.
Catalytic activity	Succinate + ubiquinone = fumarate + ubiquinol.
Cofactor	Binds 1 2Fe-2S cluster By similarity. Binds 1 3Fe-4S cluster By similarity. Binds 1 4Fe-4S cluster By similarity.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle.
Subunit structure	Component of complex II composed of four subunits: the flavoprotein (FP) SDHA, iron-sulfur protein (IP) SDHB, and a cytochrome b composed of a large and a small subunit By similarity.
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein; Matrix side.
Tissue specificity	Ubiquitous. Preferentially expressed in flowers and inflorescences.
Developmental stage	Expressed in floral meristems and sex organ primordia at early stages of development. Later expressed in anthers, particularly in the tapetum, pollen mother cells, and microspores.
Sequence similarities	Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family. Contains 1 2Fe-2S ferredoxin-type domain. Contains 1 4Fe-4S ferredoxin-type domain.

Ontologies

Keywords
Biological process	Electron transport Transport Tricarboxylic acid cycle
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Ligand	2Fe-2S 3Fe-4S 4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	succinate dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Transit peptide	1 – 28	28	Mitochondrion Potential
	Chain	29 – 279	251	Succinate dehydrogenase [ubiquinone] iron-sulfur subunit 1, mitochondrial
Regions
	Domain	52 – 141	90	2Fe-2S ferredoxin-type
	Domain	184 – 214	31	4Fe-4S ferredoxin-type
Sites
	Metal binding	102	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	107	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	110	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	122	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	194	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	197	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	200	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	204	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	251	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	257	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	261	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Binding site	209	1	Ubiquinone; shared with DHSD By similarity
Experimental info
	Sequence conflict	20	1	A → V Ref.1 Ref.4
	Sequence conflict	26	1	A → V Ref.1 Ref.4
	Sequence conflict	200	1	C → F in AAM63946. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q8LBZ7-1 [UniParc]. Last modified July 25, 2006. Version 2. Checksum: ADA885EC59A3EA36	FASTA	279	31,171
	10 20 30 40 50 60 MASGLIGRLV GTKPSKLATA ARLIPARWTS TGAEAETKAS SGGGRGSNLK TFQIYRWNPD 70 80 90 100 110 120 NPGKPELQNY QIDLKDCGPM VLDALIKIKN EMDPSLTFRR SCREGICGSC AMNIDGCNGL 130 140 150 160 170 180 ACLTKIQDEA SETTITPLPH MFVIKDLVVD MTNFYNQYKS IEPWLKRKTP ASVPAKEILQ 190 200 210 220 230 240 SKKDRAKLDG MYECILCACC STSCPSYWWN PESYLGPAAL LHANRWISDS RDEYTKERLE 250 260 270 AIDDEFKLYR CHTILNCARA CPKGLNPGKQ ITHIKQLQR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Three different genes encode the iron-sulphur subunit of succinate dehydrogenase in Arabidopsis thaliana." Figueroa P., Leon G., Elorza A., Holuigue L., Jordana X. Plant Mol. Biol. 46:241-250(2001) [PubMed: 11442063] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Strain: cv. Columbia.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S. DNA Res. 7:131-135(2000) [PubMed: 10819329] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W., Redman J.C., Wu H.C., Utterback T., Town C.D. Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"The four subunits of mitochondrial respiratory complex II are encoded by multiple nuclear genes and targeted to mitochondria in Arabidopsis thaliana." Figueroa P., Leon G., Elorza A., Holuigue L., Araya A., Jordana X. Plant Mol. Biol. 50:725-734(2002) [PubMed: 12374303] [Abstract] Cited for: SUBUNIT, TISSUE SPECIFICITY.
[7]	"Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins." Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H. Plant Cell 16:241-256(2004) [PubMed: 14671022] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Nuclear SDH2-1 and SDH2-2 genes, encoding the iron-sulfur subunit of mitochondrial complex II in Arabidopsis, have distinct cell-specific expression patterns and promoter activities." Elorza A., Leon G., Gomez I., Mouras A., Holuigue L., Araya A., Jordana X. Plant Physiol. 136:4072-4087(2004) [PubMed: 15563621] [Abstract] Cited for: DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ278910 mRNA. Translation: CAC19855.1. AB024028 Genomic DNA. Translation: BAA95713.1. AY035013 mRNA. Translation: AAK59518.1. AY063053 mRNA. Translation: AAL34227.1. AY924777 mRNA. Translation: AAX23852.1. AY086901 mRNA. Translation: AAM63946.1.
RefSeq	NP_001118718.1. NP_189374.1.
UniGene	At.72257
3D structure databases
HSSP	HSSP built from PDB template 1NEK based on UniProtKB P07014.
ModBase	Search...
Genome annotation databases
GeneID	822359.
GenomeReviews	Gene locus AT3G27380 in contig BA000014_GR.
KEGG	ath:AT3G27380.
NMPDR	fig|3702.1.peg.15028.
Organism-specific databases
GeneFarm	2168. 175.
TAIR	At3g27380.
Gene expression databases
GermOnline	AT3G27380. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR006058. 2Fe2S_fd_BS. IPR001450. 4Fe4S_Fe_S_bd. IPR012675. b-grasp_ferredoxin-like. IPR001041. Ferredoxin. IPR012285. Fum_reductase_C. IPR004489. Succ_DHase/fum_Rdtase_Fe-S. [Graphical view]
Gene3D	G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit. G3DSA:1.10.1060.10. Fum_reductase_C. 1 hit.
Pfam	PF00111. Fer2. 1 hit. [Graphical view]
TIGRFAMs	TIGR00384. dhsB. 1 hit.
PROSITE	PS00197. 2FE2S_FER_1. 1 hit. PS51085. 2FE2S_FER_2. 1 hit. PS00198. 4FE4S_FER_1. 1 hit. PS51379. 4FE4S_FER_2. 1 hit. [Graphical view]
ProDom	Q8LBZ7. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

DHSB1_ARATH

Accession

Primary (citable) accession number: Q8LBZ7
Secondary accession number(s): Q9G3M0, Q9LTZ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	July 25, 2006
Last modified:	July 22, 2008
This is version 43 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)