Reviewed, UniProtKB/Swiss-Prot Q8LEA8 (EID1_ARATH)

Names and origin

Protein names

Recommended name:
    Phytochrome A-associated F-box protein
Alternative name(s):
    Empfindlicher im dunkelroten Licht protein 1

Gene names

Name:	EID1
Ordered Locus Names:	At4g02440
ORF Names:	T14P8.22

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Negative regulator of the phyA signaling pathway that shifts the responsiveness of the phyA signaling system associated with hypocotyl elongation from red to far-red wavelength.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Probable component of an E3 ubiquitin ligase SCF complex. Interacts with SKP1A and SKP1B.
Subcellular location	Nucleus.
Domain	The F-box domain is required for the interaction with SKP1A and SKP1B.
Miscellaneous	'Empfindlicher im dunkelroten licht' means sensitive to far-red light in German.
Sequence similarities	Contains 1 F-box domain.

Ontologies

Keywords
Biological process	Phytochrome signaling pathway Ubl conjugation pathway
Cellular component	Nucleus
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SKP1A	Q39255	2	EBI-687388,EBI-532357
SKP1B	Q9FHW7	2	EBI-687388,EBI-604076

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 336	336	Phytochrome A-associated F-box protein
Regions
	Domain	3 – 55	53	F-box
	Motif	197 – 201	5	Nuclear localization signal
Experimental info
	Mutagenesis	10	1	P → A: Disrupts interaction with SKP1A and SKP1B
	Mutagenesis	101	1	G → C in eid1-2; enhances sensitivity to red and far-red light
	Mutagenesis	197 – 198	2	RK → SE: Cytoplasmic localization instead of nuclear
	Sequence conflict	16	1	N → K in AAM62754. Ref.4
	Sequence conflict	71	1	A → AA in AAM62754. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q8LEA8-1 [UniParc]. Last modified June 21, 2005. Version 2. Checksum: 0E4231CCF243487C	FASTA	336	37,398
	10 20 30 40 50 60 MAESVFSCIP EDVVFNIFFK LQDDPRNWAR LACVCTKFSS IVRNVCCKTQ CYSAIPTVIS 70 80 90 100 110 120 DLLPLPPSAA ASASSSTAAD SSLTPPGGWA SLYKLAVCCP GLFHAGILLE NSDFGLEREL 130 140 150 160 170 180 GPDQNLDPKP TTTDLALNDE EVSKPVGSGL ETTSFWSLYD DLYTDTIPAP PPEDSIDDQE 190 200 210 220 230 240 EEIETSEIRP GRDLPVRKRR KICRSLGSHL ASGGWNLSRE QGNKLLASRF RGDCLYICNW 250 260 270 280 290 300 PGCIHVEEKR NYMLFRGVFK DFKRSRVWRT INDGNRSKTS GLKCAFCLCD ETWDLHSSFC 310 320 330 LRRVFGFHDD GEPVVRAYVC ENGHVSGAWT ALPLYT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed: 10617198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"eid1: a new Arabidopsis mutant hypersensitive in phytochrome A-dependent high-irradiance responses." Bueche C., Poppe C., Schaefer E., Kretsch T. Plant Cell 12:547-558(2000) [PubMed: 10760243] [Abstract] Cited for: FUNCTION.
[6]	"EID1, an F-box protein involved in phytochrome A-specific light signaling." Dieterle M., Zhou Y.-C., Schaefer E., Funk M., Kretsch T. Genes Dev. 15:939-944(2001) [PubMed: 11316788] [Abstract] Cited for: SUBCELLULAR LOCATION, NUCLEAR LOCALIZATION SIGNAL, INTERACTION WITH SKP1A AND SKP1B, DOMAIN, MUTAGENESIS OF PRO-10; GLY-101 AND 197-ARG-LYS-198.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF069298 Genomic DNA. Translation: AAC19270.1. AL161494 Genomic DNA. Translation: CAB80737.1. BT002038 mRNA. Translation: AAN72049.1. BT008731 mRNA. Translation: AAP42744.1. AK229833 mRNA. Translation: BAF01662.1. AY085530 mRNA. Translation: AAM62754.1.
PIR	T01306.
RefSeq	NP_192153.1.
UniGene	At.43977
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8LEA8.
Genome annotation databases
GeneID	828008.
GenomeReviews	Gene locus AT4G02440 in contig CT486007_GR.
KEGG	ath:AT4G02440.
NMPDR	fig|3702.1.peg.18057.
Organism-specific databases
TAIR	At4g02440.
Gene expression databases
GermOnline	AT4G02440. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR001810. F-box. [Graphical view]
Pfam	PF00646. F-box. 1 hit. [Graphical view]
PROSITE	PS50181. FBOX. False negative. [Graphical view]
ProDom	Q8LEA8. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

EID1_ARATH

Accession

Primary (citable) accession number: Q8LEA8
Secondary accession number(s): O81289, Q0WMI9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	June 21, 2005
Last modified:	July 22, 2008
This is version 42 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)