Reviewed, UniProtKB/Swiss-Prot Q8NQ22 (SYA_CORGL)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name(s)=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	Cgl1632, cg1838

Organism

Corynebacterium glutamicum (Brevibacterium flavum) [Complete proteome] [HAMAP]

Taxonomic identifier

1718 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	888 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 888	888	Alanyl-tRNA synthetase

Sequences

	Sequence		Length	Mass (Da)
	Q8NQ22-1 [UniParc]. Last modified October 25, 2002. Version 1. Checksum: 18AE4DC95F5DDD59	FASTA	888	96,226
	10 20 30 40 50 60 MQTHEIRERF TNHFVNAGHQ AVPSASLILD DPNLLFVNAG MVPFKPYFLG QQTPPFENGT 70 80 90 100 110 120 ATSIQKCVRT LDIEEVGITT RHNTFFQMAG NFSFGQYFKE GAITHAWGLL TGSVADGGFG 130 140 150 160 170 180 LDPERLWVTV YLDDDEAAEI WEKKIGVPSE RIQRLGMADN YWSMGVPGPC GPCSEIYYDR 190 200 210 220 230 240 GEKYGKEGGP VADDNRYMEI WNLVFMEKER GQGIGKDNFD ILGDLPKKNI DTGMGVERVA 250 260 270 280 290 300 CILQDVENVY ETDLLRPVID VAETLTGTKY GSDNTSDIRF RVIADHSRTG MMLILDGVTP 310 320 330 340 350 360 GNEGRGYILR RLLRRIIRSA RLLGATGETM EQFMNTIMDT MTPSYPEIAD NRERIMRVAV 370 380 390 400 410 420 TEERAFLKTL VSGTHLFEEA ATSIKAAGST KVAGAQAFAL HDTYGFPIDL TLEMAAEAGL 430 440 450 460 470 480 EVDVEGFDSL MAEQRSRAKA DSQAKKHGHT DLSIYREWVD NNPTVFTGFE ELDSQSKVLG 490 500 510 520 530 540 LLSDGAKISE ATEGQEVEVI LDQSPLYAES GGQLGDRGQI LLGDTVLDVH DVQKIGKKLW 550 560 570 580 590 600 VHKALVANGG LAVGDEVVAS VDKQWRHAAR QAHTATHLIH AALRQVLGPT ALQAGSMNKP 610 620 630 640 650 660 GYLRFDFNYT EQLTPAQVEQ IQAITNEAVD TDWAVNTVET SLEEAKAMGA MALFGENYGS 670 680 690 700 710 720 TVRVVEIGGP FSMELCGGTH VAHSSQIGPV ALLGESSIGS GVRRIEAYSG LNSFNYLSKE 730 740 750 760 770 780 RALAEGLASS LKAPSEELPE RVAQLVDKLK AAEKEIEALH RQQLMAQTAD LLNNAQEIGG 790 800 810 820 830 840 VTTLLLRVKD NTNAGDLRTI ATTLKDKLGD REGVLVIASD NAGKVPFVVA ATKAAVARGA 850 860 870 880 HSGNLVKLVG SYIDGRGGGK ADLAQGSGAN IAGLESAFGA VRAEIEAL

References

[1]

"Complete genomic sequence of Corynebacterium glutamicum ATCC 13032." Nakagawa S. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

[2]

"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins." Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A. J. Biotechnol. 104:5-25(2003) [PubMed: 12948626] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000036 Genomic DNA. Translation: BAB99025.1. BX927152 Genomic DNA. Translation: CAF21641.1.
RefSeq	NP_600846.1. YP_225917.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1019601. 3343630.
GenomeReviews	Gene locus cg1838 in contig BX927147_GR. Gene locus Cgl1632 in contig BA000036_GR.
KEGG	cgb:cg1838. cgl:NCgl1570.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8NQ22.
Enzyme and pathway databases
BioCyc	CGLU196627-1:CG1838-MON.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProDom	Q8NQ22. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

SYA_CORGL

Accession

Primary (citable) accession number: Q8NQ22

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2002
Last sequence update:	October 25, 2002
Last modified:	July 22, 2008
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)