Reviewed, UniProtKB/Swiss-Prot Q8NZE1 (LACG_STRP8)

Names and origin

Protein names

Recommended name:
    6-phospho-beta-galactosidase
    EC=3.2.1.85
Alternative name(s):
    Beta-D-phosphogalactoside galactohydrolase
      Short name=PGALase
    P-beta-Gal
      Short name=PBG

Gene names

Name:	lacG
Ordered Locus Names:	spyM18_1984

Organism

Streptococcus pyogenes serotype M18 [Complete proteome] [HAMAP]

Taxonomic identifier

301451 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	468 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	A 6-phospho-beta-D-galactoside + H(2)O = 6-phospho-D-galactose + an alcohol.
Pathway	Carbohydrate metabolism; lactose degradation; D-galactose 6-phosphate and beta-D-glucose from lactose 6-phosphate: step 1/1.
Sequence similarities	Belongs to the glycosyl hydrolase 1 family.

Ontologies

Keywords
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	galactosidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 468	468	6-phospho-beta-galactosidase
Sites
	Active site	160	1	Proton donor By similarity
	Active site	375	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8NZE1-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 370C3623B3385D9A	FASTA	468	53,830
	10 20 30 40 50 60 MTKTLPKDFI FGGATAAYQA EGATHTDGKG PVAWDKYLED NYWYTAEPAS DFYNRYPVDL 70 80 90 100 110 120 KLSEEFGVNG IRISIAWSRI FPTGKGDVNP KGVEYYHNLF AECHKRHVEP FVTLHHFDTP 130 140 150 160 170 180 EALHSDGDFL NRENIEHFVN YAEFCFKEFS EVNYWTTFNE IGPIGDGQYL VGKFPPGIQY 190 200 210 220 230 240 DLAKVFQSHH NMMVSHARAV KLFKDGGYSG EIGVVHALPT KYPFDANNPD DVRAAELEDI 250 260 270 280 290 300 IHNKFILDAT YLGKYSDKTM EGVNHILEVN GGELDLREED FVALDAAKDL NDFLGINYYM 310 320 330 340 350 360 SDWMQAFDGE TEIIHNGKGE KGSSKYQIKG VGRRKAPVDV PKTDWDWIIF PQGLYDQIMR 370 380 390 400 410 420 VKADYPNYKK IYITENGLGY KDEFVDNTVY DDGRIDYVKK HLEVISDAIS DGANVKGYFM 430 440 450 460 WSLMDVFSWS NGYEKRYGLF YVDFETQERY PKKSAYWYKK VAETQVIE

References

[1]

"Genome sequence and comparative microarray analysis of serotype M18 group A Streptococcus strains associated with acute rheumatic fever outbreaks." Smoot J.C., Barbian K.D., Van Gompel J.J., Smoot L.M., Chaussee M.S., Sylva G.L., Sturdevant D.E., Ricklefs S.M., Porcella S.F., Parkins L.D., Beres S.B., Campbell D.S., Smith T.M., Zhang Q., Kapur V., Daly J.A., Veasy L.G., Musser J.M. Proc. Natl. Acad. Sci. U.S.A. 99:4668-4673(2002) [PubMed: 11917108] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MGAS8232 / Serotype M18.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE010103 Genomic DNA. Translation: AAL98470.1.
3D structure databases
HSSP	HSSP built from PDB template 1PBG based on UniProtKB P11546.
SMR	Q8NZE1. Positions 1-468.
ModBase	Search...
Genome annotation databases
GenomeReviews	Gene locus spyM18_1984 in contig AE009949_GR.
KEGG	spm:spyM18_1984.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8NZE1.
Enzyme and pathway databases
BioCyc	SPYO186103:SPYM18_1984-MON.
Family and domain databases
HAMAP	MF_01574. [Tree]
InterPro	IPR001360. Glyco_hydro_1. IPR013781. Glyco_hydro_sub_cat. IPR005928. LacG. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR10353. Glyco_hydro_1. 1 hit.
Pfam	PF00232. Glyco_hydro_1. 1 hit. [Graphical view]
PRINTS	PR00131. GLHYDRLASE1.
TIGRFAMs	TIGR01233. lacG. 1 hit.
PROSITE	PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit. PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit. [Graphical view]
ProDom	Q8NZE1. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

LACG_STRP8

Accession

Primary (citable) accession number: Q8NZE1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	October 1, 2002
Last modified:	September 2, 2008
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)