Reviewed, UniProtKB/Swiss-Prot Q8R455 (TRPM8_RAT)

Names and origin

Protein names

Recommended name:
    Transient receptor potential cation channel subfamily M member 8
Alternative name(s):
    Cold menthol receptor 1

Gene names

Name:	Trpm8
Synonyms:	Cmr1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	1104 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonists menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing.
Subcellular location	Membrane; Multi-pass membrane proteinProbable.
Tissue specificity	Expressed in dorsal root and trigeminal ganglia. Specifically expressed in a subset of sensory neurons, including cold-sensitive neurons in trigeminal neurons.
Sequence similarities	Belongs to the transient receptor family. LTrpC subfamily.

Ontologies

Keywords
Biological process	Ion transport Sensory transduction Transport
Cellular component	Membrane
Domain	Transmembrane
Molecular function	Ionic channel
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 1104	1104	Transient receptor potential cation channel subfamily M member 8
Regions
	Topological domain	1 – 691	691	Cytoplasmic Potential
	Transmembrane	692 – 712	21	1 Potential
	Topological domain	713 – 734	22	Extracellular Potential
	Transmembrane	735 – 755	21	2 Potential
	Topological domain	756 – 759	4	Cytoplasmic Potential
	Transmembrane	760 – 780	21	3 Potential
	Topological domain	781 – 793	13	Extracellular Potential
	Transmembrane	794 – 814	21	4 Potential
	Topological domain	815 – 829	15	Cytoplasmic Potential
	Transmembrane	830 – 850	21	5 Potential
	Topological domain	851 – 958	108	Extracellular Potential
	Transmembrane	959 – 979	21	6 Potential
	Topological domain	980 – 1104	125	Cytoplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8R455-1 [UniParc]. Last modified January 4, 2005. Version 4. Checksum: D07BFF14EB23970A	FASTA	1,104	127,629
	10 20 30 40 50 60 MSFEGARLSM RSRRNGTLGS TRTLYSSVSR STDVSYSESD LVNFIQANFK KRECVFFTRD 70 80 90 100 110 120 SKAMESICKC GYAQSQHIEG TQINQNEKWN YKKHTKEFPT DAFGDIQFET LGKKGKYLRL 130 140 150 160 170 180 SCDTDSETLY ELLTQHWHLK TPNLVISVTG GAKNFALKPR MRKIFSRLIY IAQSKGAWIL 190 200 210 220 230 240 TGGTHYGLMK YIGEVVRDNT ISRNSEENIV AIGIAAWGMV SNRDTLIRNC DDEGHFSAQY 250 260 270 280 290 300 IMDDFMRDPL YILDNNHTHL LLVDNGCHGH PTVEAKLRNQ LEKYISERTS QDSNYGGKIP 310 320 330 340 350 360 IVCFAQGGGR ETLKAINTSV KSKIPCVVVE GSGQIADVIA SLVEVEDVLT SSMVKEKLVR 370 380 390 400 410 420 FLPRTVSRLP EEEIESWIKW LKEILESPHL LTVIKMEEAG DEVVSSAISY ALYKAFSTNE 430 440 450 460 470 480 QDKDNWNGQL KLLLEWNQLD LASDEIFTND RRWESADLQE VMFTALIKDR PKFVRLFLEN 490 500 510 520 530 540 GLNLQKFLTN EVLTELFSTH FSTLVYRNLQ IAKNSYNDAL LTFVWKLVAN FRRSFWKEDR 550 560 570 580 590 600 SSREDLDVEL HDASLTTRHP LQALFIWAIL QNKKELSKVI WEQTKGCTLA ALGASKLLKT 610 620 630 640 650 660 LAKVKNDINA AGESEELANE YETRAVELFT ECYSSDEDLA EQLLVYSCEA WGGSNCLELA 670 680 690 700 710 720 VEATDQHFIA QPGVQNFLSK QWYGEISRDT KNWKIILCLF IIPLVGCGLV SFRKKPIDKH 730 740 750 760 770 780 KKLLWYYVAF FTSPFVVFSW NVVFYIAFLL LFAYVLLMDF HSVPHTPELI LYALVFVLFC 790 800 810 820 830 840 DEVRQWYMNG VNYFTDLWNV MDTLGLFYFI AGIVFRLHSS NKSSLYSGRV IFCLDYIIFT 850 860 870 880 890 900 LRLIHIFTVS RNLGPKIIML QRMLIDVFFF LFLFAVWMVA FGVARQGILR QNEQRWRWIF 910 920 930 940 950 960 RSVIYEPYLA MFGQVPSDVD STTYDFSHCT FSGNESKPLC VELDEYNLPR FPEWITIPLV 970 980 990 1000 1010 1020 CIYMLSTNIL LVNLLVAMFG YTVGIVQENN DQVWKFQRYF LVQEYCNRLN IPFPFVVFAY 1030 1040 1050 1060 1070 1080 FYMVVKKCFK CCCKEKNTES SACCFRNEDN ETLAWEGVMK ENYLVKINTK ANDNAEEMRH 1090 1100 RFRQLDTKLN DLKGLLKEIA NKIK

References

[1]	"Identification of a cold receptor reveals a general role for TRP channels in thermosensation." McKemy D.D., Neuhausser W.M., Julius D. Nature 416:52-58(2002) [PubMed: 11882888] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY. Tissue: Trigeminal ganglion.
[2]	McKemy D.D., Neuhausser W.M., Julius D. Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 449. Tissue: Trigeminal ganglion.
[3]	"TRPM8 mRNA is expressed in a subset of cold-responsive trigeminal neurons from rat." Nealen M.L., Gold M.S., Thut P.D., Caterina M.J. J. Neurophysiol. 90:515-520(2003) [PubMed: 12634279] [Abstract] Cited for: TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY072788 mRNA. Translation: AAL68394.2.
RefSeq	NP_599198.2.
UniGene	Rn.81225
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSRNOG00000019035. Rattus norvegicus. [Contig view]
GeneID	171384.
KEGG	rno:171384.
NMPDR	fig|10116.3.peg.30442.
Organism-specific databases
RGD	620762. Trpm8.
Phylogenomic databases
HOVERGEN	Q8R455.
Gene expression databases
ArrayExpress	Q8R455.
GermOnline	ENSRNOG00000019035. Rattus norvegicus.
Family and domain databases
InterPro	IPR005821. Ion_trans. [Graphical view]
Pfam	PF00520. Ion_trans. 1 hit. [Graphical view]
ProDom	Q8R455. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

TRPM8_RAT

Accession

Primary (citable) accession number: Q8R455

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 21, 2004
Last sequence update:	January 4, 2005
Last modified:	July 22, 2008
This is version 37 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)