Reviewed, UniProtKB/Swiss-Prot Q8R4D5 (TRPM8_MOUSE)

Names and origin

Protein names

Recommended name:
    Transient receptor potential cation channel subfamily M member 8
Alternative name(s):
    Long transient receptor potential channel 6
      Short name(s)=LTrpC6
    Transient receptor potential-p8
      Short name(s)=Trp-p8

Gene names

Name:	Trpm8
Synonyms:	Ltrpc6, Trpp8

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	1104 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonists menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing.
Subcellular location	Membrane; Multi-pass membrane proteinProbable.
Tissue specificity	Expressed in dorsal root and trigeminal ganglia. Specifically expressed in a subset of pain- and temperature-sensing neurons. Not expressed in heavily myelinated neurons. Not expressed in neurons expressing TRPA1 or TRPV1.
Sequence similarities	Belongs to the transient receptor family. LTrpC subfamily.

Ontologies

Keywords
Biological process	Ion transport Sensory transduction Transport
Cellular component	Membrane
Domain	Transmembrane
Molecular function	Ionic channel
Gene Ontology (GO)
Biological process	cellular calcium ion homeostasis Ref.1 Inferred from direct assay. Source: MGI response to cold Ref.3 Inferred from direct assay. Source: MGI thermoception Ref.3 Inferred from direct assay. Source: MGI
Cellular component	membrane Ref.1 Inferred from direct assay. Source: MGI
Molecular function	calcium channel activity Ref.1 Ref.3 Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 1104	1104	Transient receptor potential cation channel subfamily M member 8
Regions
	Topological domain	1 – 691	691	Cytoplasmic Potential
	Transmembrane	692 – 712	21	1 Potential
	Topological domain	713 – 734	22	Extracellular Potential
	Transmembrane	735 – 755	21	2 Potential
	Topological domain	756 – 759	4	Cytoplasmic Potential
	Transmembrane	760 – 780	21	3 Potential
	Topological domain	781 – 794	14	Extracellular Potential
	Transmembrane	795 – 815	21	4 Potential
	Topological domain	816 – 829	14	Cytoplasmic Potential
	Transmembrane	830 – 850	21	5 Potential
	Topological domain	851 – 958	108	Extracellular Potential
	Transmembrane	959 – 979	21	6 Potential
	Topological domain	980 – 1104	125	Cytoplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8R4D5-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 16B5D6FBED2BE96D	FASTA	1,104	127,710
	10 20 30 40 50 60 MSFEGARLSM RSRRNGTMGS TRTLYSSVSR STDVSYSDSD LVNFIQANFK KRECVFFTRD 70 80 90 100 110 120 SKAMENICKC GYAQSQHIEG TQINQNEKWN YKKHTKEFPT DAFGDIQFET LGKKGKYLRL 130 140 150 160 170 180 SCDTDSETLY ELLTQHWHLK TPNLVISVTG GAKNFALKPR MRKIFSRLIY IAQSKGAWIL 190 200 210 220 230 240 TGGTHYGLMK YIGEVVRDNT ISRNSEENIV AIGIAAWGMV SNRDTLIRSC DDEGHFSAQY 250 260 270 280 290 300 IMDDFTRDPL YILDNNHTHL LLVDNGCHGH PTVEAKLRNQ LEKYISERTS QDSNYGGKIP 310 320 330 340 350 360 IVCFAQGGGR ETLKAINTSV KSKIPCVVVE GSGQIADVIA SLVEVEDVLT SSMVKEKLVR 370 380 390 400 410 420 FLPRTVSRLP EEEIESWIKW LKEILESSHL LTVIKMEEAG DEIVSNAISY ALYKAFSTNE 430 440 450 460 470 480 QDKDNWNGQL KLLLEWNQLD LASDEIFTND RRWESADLQE VMFTALIKDR PKFVRLFLEN 490 500 510 520 530 540 GLNLQKFLTN EVLTELFSTH FSTLVYRNLQ IAKNSYNDAL LTFVWKLVAN FRRSFWKEDR 550 560 570 580 590 600 SSREDLDVEL HDASLTTRHP LQALFIWAIL QNKKELSKVI WEQTKGCTLA ALGASKLLKT 610 620 630 640 650 660 LAKVKNDINA AGESEELANE YETRAVELFT ECYSNDEDLA EQLLVYSCEA WGGSNCLELA 670 680 690 700 710 720 VEATDQHFIA QPGVQNFLSK QWYGEISRDT KNWKIILCLF IIPLVGCGLV SFRKKPIDKH 730 740 750 760 770 780 KKLLWYYVAF FTSPFVVFSW NVVFYIAFLL LFAYVLLMDF HSVPHTPELI LYALVFVLFC 790 800 810 820 830 840 DEVRQWYMNG VNYFTDLWNV MDTLGLFYFI AGIVFRLHSS NKSSLYSGRV IFCLDYIIFT 850 860 870 880 890 900 LRLIHIFTVS RNLGPKIIML QRMLIDVFFF LFLFAVWMVA FGVARQGILR QNEQRWRWIF 910 920 930 940 950 960 RSVIYEPYLA MFGQVPSDVD STTYDFSHCT FSGNESKPLC VELDEHNLPR FPEWITIPLV 970 980 990 1000 1010 1020 CIYMLSTNIL LVNLLVAMFG YTVGIVQENN DQVWKFQRYF LVQEYCNRLN IPFPFVVFAY 1030 1040 1050 1060 1070 1080 FYMVVKKCFK CCCKEKNMES NACCFRNEDN ETLAWEGVMK ENYLVKINTK ANDNSEEMRH 1090 1100 RFRQLDSKLN DLKSLLKEIA NNIK

References

[1]	"A TRP channel that senses cold stimuli and menthol." Peier A.M., Moqrich A., Hergarden A.C., Reeve A.J., Andersson D.A., Story G.M., Earley T.J., Dragon I., McIntyre P., Bevan S., Patapoutian A. Cell 108:705-715(2002) [PubMed: 11893340] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, PROBABLE SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Strain: C57BL/6. Tissue: Spinal ganglion.
[2]	"The murine homologue of TRPM8(Trpp8) gene: cloning, sequencing and tissue distribution." Tsavaler L., Laus R. Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Testis.
[3]	"ANKTM1, a TRP-like channel expressed in nociceptive neurons, is activated by cold temperatures." Story G.M., Peier A.M., Reeve A.J., Eid S.R., Mosbacher J., Hricik T.R., Earley T.J., Hergarden A.C., Anderson D.A., Hwang S.W., McIntyre P., Jegla T., Bevan S., Patapoutian A. Cell 112:819-829(2003) [PubMed: 12654248] [Abstract] Cited for: TISSUE SPECIFICITY.
[4]	"TRPM8 activation by menthol, icilin, and cold is differentially modulated by intracellular pH." Andersson D.A., Chase H.W., Bevan S. J. Neurosci. 24:5364-5369(2004) [PubMed: 15190109] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF481480 mRNA. Translation: AAL79553.1. AY095352 mRNA. Translation: AAM23261.1.
RefSeq	NP_599013.1.
UniGene	Mm.218753
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSMUSG00000036251. Mus musculus. [Contig view]
GeneID	171382.
KEGG	mmu:171382.
Organism-specific databases
MGI	MGI:2181435. Trpm8.
Phylogenomic databases
HOGENOM	Q8R4D5.
HOVERGEN	Q8R4D5.
Gene expression databases
ArrayExpress	Q8R4D5.
GermOnline	ENSMUSG00000036251. Mus musculus.
Family and domain databases
InterPro	IPR005821. Ion_trans. [Graphical view]
Pfam	PF00520. Ion_trans. 1 hit. [Graphical view]
ProDom	Q8R4D5. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
SOURCE	Search...
ProtoNet	Search...

Entry information

Entry name

TRPM8_MOUSE

Accession

Primary (citable) accession number: Q8R4D5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 21, 2004
Last sequence update:	June 1, 2002
Last modified:	July 22, 2008
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)