Reviewed, UniProtKB/Swiss-Prot Q8UE87 (SYA_AGRT5)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	Atu1873
ORF Names:	AGR_C_3437

Organism

Agrobacterium tumefaciens (strain C58 / ATCC 33970) [Complete proteome] [HAMAP]

Taxonomic identifier

176299 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	887 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Domain	Zinc-finger
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 887	887	Alanyl-tRNA synthetase
Regions
	Zinc finger	169 – 182	14	C2H2-type

Sequences

	Sequence		Length	Mass (Da)
	Q8UE87-1 [UniParc]. Last modified August 2, 2002. Version 2. Checksum: 4AB5CDB1C58A4238	FASTA	887	95,738
	10 20 30 40 50 60 MSGVNEIRST FLDYFKKNGH EIVPSSPLVP RNDPTLMFTN AGMVQFKNVF TGLESRPYST 70 80 90 100 110 120 AASAQKCVRA GGKHNDLDNV GYTARHHTFF EMLGNFSFGD YFKEEAITHA WNLITKEFGI 130 140 150 160 170 180 DRNRLLVTVY HTDDEAFNLW KKIAGFSDDR IIRIPTSDNF WAMGDTGPCG PCSEIFYDHG 190 200 210 220 230 240 DHIWGGPPGS PEEDGDRFIE IWNLVFMQYE QLTKEERIDL PRPSIDTGMG LERISALLQG 250 260 270 280 290 300 KHDNYDTDLF RALIAASVEA TGVPAEGEHR ASHRVIADHL RSSAFLIADG VLPSSEGRGY 310 320 330 340 350 360 VLRRIMRRAM RHAELLGSRD PLIYRLLPAL IQQMGRAYPE LVRAEALISE TLKLEETRFR 370 380 390 400 410 420 KTLERGLSLL SDATSTLHKG DMLDGETAFK LYDTYGFPLD LTQDALRARE IGVDISGFTD 430 440 450 460 470 480 AMQRQKAEAR SHWAGSGDKA TETVWFELKE KFGATEFLGY DTESAEGVIQ AIVRDGKEVD 490 500 510 520 530 540 SAAEGETVHI VVNQTPFYGE SGGQMGDTGV IVGDAGTFDV SGTQKKGEGL FVHSGTVSKG 550 560 570 580 590 600 GLKVNEAVQL TVDHDRRSRL RANHSATHLL HEALREVLGT HVAQKGSLVA PERLRFDVSH 610 620 630 640 650 660 PKPMSAEELK VVEDMANEIV LQNSPVVTRL MSVDDAIAEG AMALFGEKYG DEVRVVSMGT 670 680 690 700 710 720 GLHGAKANRP YSVELCGGTH VAATGQIGLI RILGESAVGS GVRRLEAVTG QGALAYLAEQ 730 740 750 760 770 780 DERVKALASS LKVQPGDVLS RVEGLLDERK KLERELADAR KKLAMGGGSS DAGANDVQQV 790 800 810 820 830 840 AGVNFLAKSL SGIDAKDLKG LADEAKANLG SGVVLLIAVS DDGKASAVAA VTEDLTGRFS 850 860 870 880 AVDIVRTASA ALGGKGGGGR PDMAQAGGPD GTKAKEAIEA VAAALAA

References

[1]

"The genome of the natural genetic engineer Agrobacterium tumefaciens C58." Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J. , Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R., Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G., Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y., Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S., Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F., Gordon M.P., Olson M.V., Nester E.W. Science 294:2317-2323(2001) [PubMed: 11743193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58." Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C. , Allinger M., Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C., Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S. Science 294:2323-2328(2001) [PubMed: 11743194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE007869 Genomic DNA. Translation: AAK87639.2.
PIR	AG2806. F97585.
RefSeq	NP_354854.2.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1133911.
GenomeReviews	Gene locus Atu1873 in contig AE007869_GR.
KEGG	atc:AGR_C_3437. atu:Atu1873.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8UE87.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProDom	Q8UE87. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

SYA_AGRT5

Accession

Primary (citable) accession number: Q8UE87

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 2, 2002
Last sequence update:	August 2, 2002
Last modified:	July 22, 2008
This is version 44 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)