Reviewed, UniProtKB/Swiss-Prot Q8WXI4 (ACO11_HUMAN)

Names and origin

Protein names

Recommended name:
    Acyl-coenzyme A thioesterase 11
      Short name=Acyl-CoA thioesterase 11
    EC=3.1.2.-
Alternative name(s):
    Acyl-CoA thioester hydrolase 11
    Brown fat-inducible thioesterase
      Short name=BFIT
    Adipose-associated thioesterase

Gene names

Name:	ACOT11
Synonyms:	BFIT, KIAA0707, THEA

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	607 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Has acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates.
Subcellular location	Cytoplasm.
Tissue specificity	Isoform 1 is predominantly expressed in skeletal muscle, liver, testis, stomach, spleen, lung and brain. Isoform 2 is predominantly expressed in kidney, uterus, hibernoma and white adipose tissue.
Induction	By cold exposure and repressed by heat exposure.
Sequence similarities	Contains 2 acyl coenzyme A hydrolase domains. Contains 1 START domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat
Molecular function	Hydrolase Serine esterase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	fatty acid metabolic process Ref.1 Non-traceable author statement. Source: UniProtKB intracellular signaling cascade Ref.1 Non-traceable author statement. Source: UniProtKB response to temperature stimulus Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	cytoplasm Ref.1 Inferred by curator. Source: UniProtKB
Molecular function	acyl-CoA thioesterase activity Ref.1 Traceable author statement. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8WXI4-1)

Also known as: BFIT1;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8WXI4-2)

Also known as: BFIT2;

The sequence of this isoform differs from the canonical sequence as follows:
     544-607: CCWVRVSLTE...NSRFGYLQDT → VSYYNQATPG...NDLAPSLQTL

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 607	607	Acyl-coenzyme A thioesterase 11
Regions
	Domain	29 – 164	136	Acyl coenzyme A hydrolase 1
	Domain	205 – 336	132	Acyl coenzyme A hydrolase 2
	Domain	375 – 585	211	START
Amino acid modifications
	Modified residue	367	1	Phosphoserine
Natural variations
	Alternative sequence	544 – 607	64	CCWVR…YLQDT → VSYYNQATPGVLNYVTTNVA GLSSEFYTTFKACEQFLLDN RNDLAPSLQTL in isoform 2.
	Natural variant	165	1	P → L: dbSNP rs2304306.
	Natural variant	202	1	G → D: dbSNP rs1702003.
	Natural variant	212	1	M → I: dbSNP rs2304305.
Experimental info
	Sequence conflict	255	1	S → R Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (BFIT1) [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 12F2BCAB8AAC18EC	FASTA	607	68,492
	10 20 30 40 50 60 MIQNVGNHLR RGLASVFSNR TSRKSALRAG NDSAMADGEG YRNPTEVQMS QLVLPCHTNQ 70 80 90 100 110 120 RGELSVGQLL KWIDTTACLS AERHAGCPCV TASMDDIYFE HTISVGQVVN IKAKVNRAFN 130 140 150 160 170 180 SSMEVGIQVA SEDLCSEKQW NVCKALATFV ARREITKVKL KQITPRTEEE KMEHSVAAER 190 200 210 220 230 240 RRMRLVYADT IKDLLANCAI QGDLESRDCS RMVPAEKTRV ESVELVLPPH ANHQGNTFGG 250 260 270 280 290 300 QIMAWMENVA TIAASRLCRA HPTLKAIEMF HFRGPSQVGD RLVLKAIVNN AFKHSMEVGV 310 320 330 340 350 360 CVEAYRQEAE THRRHINSAF MTFVVLDADD QPQLLPWIRP QPGDGERRYR EASARKKIRL 370 380 390 400 410 420 DRKYIVSCKQ TEVPLSVPWD PSNQVYLSYN NVSSLKMLVA KDNWVLSSEI SQVRLYTLED 430 440 450 460 470 480 DKFLSFHMEM VVHVDAAQAF LLLSDLRQRP EWDKHYRSVE LVQQVDEDDA IYHVTSPALG 490 500 510 520 530 540 GHTKPQDFVI LASRRKPCDN GDPYVIALRS VTLPTHRETP EYRRGETLCS GFCLWREGDQ 550 560 570 580 590 600 LTKCCWVRVS LTELVSASGF YSWGLESRSK GRRSDGWNGK LAGGHLSTLK AIPVAKINSR FGYLQDT
	Isoform 2 (BFIT2) [UniParc]. Checksum: C9CD42FB285A8B53		594	67,152
	10 20 30 40 50 60 MIQNVGNHLR RGLASVFSNR TSRKSALRAG NDSAMADGEG YRNPTEVQMS QLVLPCHTNQ 70 80 90 100 110 120 RGELSVGQLL KWIDTTACLS AERHAGCPCV TASMDDIYFE HTISVGQVVN IKAKVNRAFN 130 140 150 160 170 180 SSMEVGIQVA SEDLCSEKQW NVCKALATFV ARREITKVKL KQITPRTEEE KMEHSVAAER 190 200 210 220 230 240 RRMRLVYADT IKDLLANCAI QGDLESRDCS RMVPAEKTRV ESVELVLPPH ANHQGNTFGG 250 260 270 280 290 300 QIMAWMENVA TIAASRLCRA HPTLKAIEMF HFRGPSQVGD RLVLKAIVNN AFKHSMEVGV 310 320 330 340 350 360 CVEAYRQEAE THRRHINSAF MTFVVLDADD QPQLLPWIRP QPGDGERRYR EASARKKIRL 370 380 390 400 410 420 DRKYIVSCKQ TEVPLSVPWD PSNQVYLSYN NVSSLKMLVA KDNWVLSSEI SQVRLYTLED 430 440 450 460 470 480 DKFLSFHMEM VVHVDAAQAF LLLSDLRQRP EWDKHYRSVE LVQQVDEDDA IYHVTSPALG 490 500 510 520 530 540 GHTKPQDFVI LASRRKPCDN GDPYVIALRS VTLPTHRETP EYRRGETLCS GFCLWREGDQ 550 560 570 580 590 LTKVSYYNQA TPGVLNYVTT NVAGLSSEFY TTFKACEQFL LDNRNDLAPS LQTL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"BFIT, a unique acyl-CoA thioesterase induced in thermogenic brown adipose tissue: cloning, organization of the human gene and assessment of a potential link to obesity." Adams S.H., Chui C., Schilbach S.L., Yu X.X., Goddard A.D., Grimaldi J.C., Lee J., Dowd P., Colman S., Lewin D.A. Biochem. J. 360:135-142(2001) [PubMed: 11696000] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
[2]	"Prediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro." Ishikawa K., Nagase T., Suyama M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 5:169-176(1998) [PubMed: 9734811] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Thyroid.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 35-255. Tissue: Skin.
[5]	"Quantitative phosphoproteome profiling of Wnt3a-mediated signaling network: indicating the involvement of ribonucleoside-diphosphate reductase M2 subunit phosphorylation at residue serine 20 in canonical Wnt signal transduction." Tang L.-Y., Deng N., Wang L.-S., Dai J., Wang Z.-L., Jiang X.-S., Li S.-J., Li L., Sheng Q.-H., Wu D.-Q., Li L., Zeng R. Mol. Cell. Proteomics 6:1952-1967(2007) [PubMed: 17693683] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-367, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF416921 mRNA. Translation: AAL40937.1. AF416922 mRNA. Translation: AAL40938.1. AB014607 mRNA. Translation: BAA31682.1. Different initiation. AK023937 mRNA. Translation: BAB14734.1. BC001517 mRNA. Translation: AAH01517.1.
PIR	T00351.
RefSeq	NP_056362.1. NP_671517.1.
UniGene	Hs.234786
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	Q8WXI4.
Genome annotation databases
Ensembl	ENSG00000162390. Homo sapiens. [Contig view]
GeneID	26027.
KEGG	hsa:26027.
Organism-specific databases
HGNC	HGNC:18156. ACOT11.
MIM	606803. gene.
PharmGKB	PA38303.
HUGE	Search...
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	Q8WXI4.
HOVERGEN	Q8WXI4.
Gene expression databases
ArrayExpress	Q8WXI4.
CleanEx	HS_ACOT11.
GermOnline	ENSG00000162390. Homo sapiens.
Family and domain databases
InterPro	IPR002913. START_lipid_bd. IPR006683. Thioestr_supf. [Graphical view]
Pfam	PF03061. 4HBT. 2 hits. PF01852. START. 1 hit. [Graphical view]
SMART	SM00234. START. 1 hit. [Graphical view]
PROSITE	PS50848. START. 1 hit. [Graphical view]
ProDom	Q8WXI4. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
SOURCE	Search...
ProtoNet	Search...

Entry information

Entry name

ACO11_HUMAN

Accession

Primary (citable) accession number: Q8WXI4
Secondary accession number(s): O75187, Q96DI1, Q9H883

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2002
Last sequence update:	March 1, 2002
Last modified:	July 22, 2008
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)