Reviewed, UniProtKB/Swiss-Prot Q8X3W8 (SYA_ECO57)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	Z3999, ECs3554

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	876 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Domain	Zinc-finger
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 876	876	Alanyl-tRNA synthetase
Regions
	Zinc finger	179 – 192	14	C2H2-type
	Region	1 – 461	461	Catalytic
Experimental info
	Sequence conflict	856 – 876	21	DAAAL…SAKLQ → GCCGLTCSVSQCERLGQREI AII in AAG57801. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q8X3W8-1 [UniParc]. Last modified May 10, 2002. Version 2. Checksum: 676A2EE7D57F9776	FASTA	876	96,032
	10 20 30 40 50 60 MSKSTAEIRQ AFLDFFHSKG HQVVASSSLV PHNDPTLLFT NAGMNQFKDV FLGLDKRNYS 70 80 90 100 110 120 RATTSQRCVR AGGKHNDLEN VGYTARHHTF FEMLGNFSFG DYFKHDAIQF AWELLTSEKW 130 140 150 160 170 180 FALPKERLWV TVYESDDEAY EIWEKEVGIP RERIIRIGDN KGAPYASDNF WQMGDTGPCG 190 200 210 220 230 240 PCTEIFYDHG DHIWGGPPGS PEEDGDRYIE IWNIVFMQFN RQADGTMEPL PKPSVDTGMG 250 260 270 280 290 300 LERIAAVLQH VNSNYDIDLF RTLIQAVAKV TGATDLSNKS LRVIADHIRS CAFLIADGVM 310 320 330 340 350 360 PSNENRGYVL RRIIRRAVRH GNMLGAKETF FYKLVGPLID VMGSAGEDLK RQQAQVEQVL 370 380 390 400 410 420 KTEEEQFART LERGLALLDE ELAKLSGDTL DGETAFRLYD TYGFPVDLTA DVCRERNIKV 430 440 450 460 470 480 DEAGFEAAME EQRRRAREAS GFGADYNAMI RVDSASEFKG YDHLELNGKV TALFVDGKAV 490 500 510 520 530 540 DAINAGQEAV VVLDQTPFYA ESGGQVGDKG ELKGANFSFV VEDTQKYGQA IGHIGKLAAG 550 560 570 580 590 600 SLKVGDAVQA DVDEARRARI RLNHSATHLM HAALRQVLGT HVSQKGSLVN DKVLRFDFSH 610 620 630 640 650 660 NEAMKPEEIR AVEDLVNAQI RRNLPIETNI MDLEAAKAKG AMALFGEKYD ERVRVLSMGD 670 680 690 700 710 720 FSTELCGGTH ASRTGDIGLF RIISESGTAA GVRRIEAVTG EGAITTVHAD SDRLSEVAHL 730 740 750 760 770 780 LKGDSNNLAD KVRSVLERTR QLEKELQQLK EQAAAQESAN LSSKAIDVNG VKLLVSELSG 790 800 810 820 830 840 VEPKMLRTMV DDLKNQLGST IIVLATVAEG KVSLIAGVSK DVTDRVKAGE LIGMVAQQVG 850 860 870 GKGGGRPDMA QAGGTDAAAL PAALASVKGW VSAKLQ

References

[1]

"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed: 11206551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.

[2]

"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed: 11258796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005174 Genomic DNA. Translation: AAG57801.1. BA000007 Genomic DNA. Translation: BAB36977.1.
PIR	B91073. E85917.
RefSeq	NP_289243.1. NP_311581.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	914724. 958243.
GenomeReviews	Gene locus ECs3554 in contig BA000007_GR. Gene locus Z3999 in contig AE005174_GR.
KEGG	ece:Z3999. ecs:ECs3554.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8X3W8.
Enzyme and pathway databases
BioCyc	ECOL83334:ECS3554-MON.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProDom	Q8X3W8. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

SYA_ECO57

Accession

Primary (citable) accession number: Q8X3W8
Secondary accession number(s): Q8X3D4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2002
Last sequence update:	May 10, 2002
Last modified:	July 22, 2008
This is version 43 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)