Reviewed, UniProtKB/Swiss-Prot Q920H4 (ACM5_MOUSE)

Names and origin

Protein names

Recommended name:
    Muscarinic acetylcholine receptor M5

Gene names

Name:

Chrm5

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	532 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
Domain	Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein Phosphoprotein
Gene Ontology (GO)
Biological process	dopamine transport Inferred from direct assay. Source: MGI transmission of nerve impulse Inferred from direct assay. Source: MGI
Molecular function	glycine-inhibited chloride channel activity Traceable author statement. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 532	532	Muscarinic acetylcholine receptor M5
Regions
	Topological domain	1 – 29	29	Extracellular Potential
	Transmembrane	30 – 53	24	1 Potential
	Topological domain	54 – 66	13	Cytoplasmic Potential
	Transmembrane	67 – 87	21	2 Potential
	Topological domain	88 – 104	17	Extracellular Potential
	Transmembrane	105 – 126	22	3 Potential
	Topological domain	127 – 146	20	Cytoplasmic Potential
	Transmembrane	147 – 169	23	4 Potential
	Topological domain	170 – 191	22	Extracellular Potential
	Transmembrane	192 – 214	23	5 Potential
	Topological domain	215 – 443	229	Cytoplasmic Potential
	Transmembrane	444 – 464	21	6 Potential
	Topological domain	465 – 478	14	Extracellular Potential
	Transmembrane	479 – 498	20	7 Potential
	Topological domain	499 – 532	34	Cytoplasmic Potential
Amino acid modifications
	Modified residue	501	1	Phosphothreonine Potential
	Modified residue	505	1	Phosphothreonine Potential
	Glycosylation	8	1	N-linked (GlcNAc...) Potential
	Disulfide bond	103 ↔ 183		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q920H4-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 181254ED8B6F6D78	FASTA	532	60,343
	10 20 30 40 50 60 MEGESYHNET TVNGTPVNHQ ALERHGLWEV ITIAAVTAVV SLMTIVGNVL VMISFKVNSQ 70 80 90 100 110 120 LKTVNNYYLL SLACADLIIG IFSMNLYTTY ILMGRWVLGS LACDLWLALD YVASNASVMN 130 140 150 160 170 180 LLVISFDRYF SITRPLTYRA KRTPKRAGIM IGLAWLVSFI LWAPAILCWQ YLVGKRTVPP 190 200 210 220 230 240 DECQIQFLSE PTITFGTAIA AFYIPVSVMT ILYCRIYRET EKRTKDLADL QGSDSVAEVK 250 260 270 280 290 300 KRKPAHRTLL RSFFSCPRPS LAQRERNQAS WSSSRRSTST TGKPTQATDL SADWEKAEQV 310 320 330 340 350 360 TNCSSCPSSE DEAKATTDPV FQVVYKNEAK ESPGKEFNTQ ETKETFVSPR TENNDYDTPK 370 380 390 400 410 420 YFLSPGAAHR LKSQKCVAYK FRLVVKADGT QETNNGCRKV KIMPCSFPVS KDPSTKGLDP 430 440 450 460 470 480 HLSHQMTKRK RMVLVKERKA AQTLSAILLA FIITWTPYNI MVLVSTFCDK CVPVTLWHLG 490 500 510 520 530 YWLCYVNSTI NPICYALCNR TFRKTFKLLL LCRWKKKKVE EKLYWQGNSK LP

References

[1]	"Isolation, sequence and functional expression of the mouse M1-M5 muscarinic acetylcholine receptor genes." Yamada M., Wess J. Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF264051 Genomic DNA. Translation: AAL26028.1.
RefSeq	NP_991352.1.
UniGene	Mm.297624
3D structure databases
HSSP	HSSP built from PDB template 1HLL based on UniProtKB P08913.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
Genome annotation databases
Ensembl	ENSMUSG00000074939. Mus musculus. [Contig view]
GeneID	213788.
KEGG	mmu:213788.
Organism-specific databases
MGI	MGI:109248. Chrm5.
Phylogenomic databases
HOGENOM	Q920H4.
HOVERGEN	Q920H4.
Gene expression databases
ArrayExpress	Q920H4.
CleanEx	MM_CHRM5.
GermOnline	ENSMUSG00000074939. Mus musculus.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_supfam. IPR000502. Musac_M5_rcpt. IPR000995. Musac_rcpt. [Graphical view]
PANTHER	PTHR19266:SF36. Musac_M5_rcpt. 1 hit.
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00243. MUSCARINICR. PR00542. MUSCRINICM5R.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProDom	Q920H4. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
SOURCE	Search...
ProtoNet	Search...

Entry information

Entry name

ACM5_MOUSE

Accession

Primary (citable) accession number: Q920H4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	December 1, 2001
Last modified:	July 22, 2008
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)