Reviewed, UniProtKB/Swiss-Prot Q92316 (OST5_YEAST)

Names and origin

Protein names

Recommended name:
    Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST5
      Short name(s)=Oligosaccharyl transferase subunit OST5
    EC=2.4.1.119
Alternative name(s):
    Oligosaccharyl transferase subunit zeta
    Oligosaccharyl transferase 9.5 kDa subunit

Gene names

Name:	OST5
Ordered Locus Names:	YGL226C-A
ORF Names:	YGL226BC

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	86 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Essential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.
Catalytic activity	Dolichyl diphosphooligosaccharide + protein L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L-asparagine.
Pathway	Protein modification; protein glycosylation.
Subunit structure	Component of the oligosaccharyl transferase (OST) complex, which apears to exist in two assemblies comprising OST1, OST2, OST4, OST5, STT3, SWP1, WPB1, and either OST3 or OST6. OST1 and OST5 probably form a subcomplex. Ma interact directly with OST1, OST2 and OST4.
Subcellular location	Membrane; Multi-pass membrane proteinPotential.
Miscellaneous	Present with 1010 molecules/cell in log phase SD medium.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid N-linked glycosylation Ref.4 Inferred from physical interaction. Source: SGD
Cellular component	oligosaccharyl transferase complex Ref.4 Ref.7 Inferred from physical interaction. Source: SGD
Molecular function	dolichyl-diphosphooligosaccharide-protein glycotransferase activity Ref.4 Inferred from physical interaction. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 86	86	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST5
Regions
	Topological domain	1 – 27	27	Cytoplasmic Potential
	Transmembrane	28 – 48	21	Potential
	Transmembrane	57 – 77	21	Potential
	Topological domain	78 – 86	9	Cytoplasmic Potential
Experimental info
	Sequence conflict	46	1	A → T in CAA66147. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q92316-1 [UniParc]. Last modified May 10, 2005. Version 2. Checksum: CAA1914962819AD6	FASTA	86	9,517
	10 20 30 40 50 60 MTYEQLYKEF HSSKSFQPFI HLDTQPKFAI CGLIVTLAVL SSALFAVGSK SSYIKKLFFY 70 80 TILSVIGSLF AGLTTVFASN SFGVYV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A specific screen for oligosaccharyltranferase mutations identifies the 9 kDa OST5 protein required for optimal acitivty in vivo and in vitro." Reiss G., te Heesen S., Gilmore R., Zufferey R., Aebi M. EMBO J. 16:1164-1172(1997) [PubMed: 9135133] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"The Saccharomyces cerevisiae oligosaccharyltransferase is a protein complex composed of Wbp1p, Swp1p, and four additional polypeptides." Kelleher D.J., Gilmore R. J. Biol. Chem. 269:12908-12917(1994) [PubMed: 8175708] [Abstract] Cited for: IDENTIFICATION IN THE OLIGOSACCHARYL TRANSFERASE COMPLEX.
[4]	"The highly conserved Stt3 protein is a subunit of the yeast oligosaccharyltransferase and forms a subcomplex with Ost3p and Ost4p." Karaoglu D., Kelleher D.J., Gilmore R. J. Biol. Chem. 272:32513-32520(1997) [PubMed: 9405463] [Abstract] Cited for: IDENTIFICATION IN THE OLIGOSACCHARYL TRANSFERASE COMPLEX.
[5]	"The oligosaccharyltransferase complex from yeast." Knauer R., Lehle L. Biochim. Biophys. Acta 1426:259-273(1999) [PubMed: 9878773] [Abstract] Cited for: REVIEW ON OLIGOSACCHARYL TRANSFERASE.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Yeast oligosaccharyltransferase consists of two functionally distinct sub-complexes, specified by either the Ost3p or Ost6p subunit." Schwarz M., Knauer R., Lehle L. FEBS Lett. 579:6564-6568(2005) [PubMed: 16297388] [Abstract] Cited for: COMPOSITION OF OLIGOSACCHARYL TRANSFERASE COMPLEXES.
[8]	"The 3.4-kDa Ost4 protein is required for the assembly of two distinct oligosaccharyltransferase complexes in yeast." Spirig U., Bodmer D., Wacker M., Burda P., Aebi M. Glycobiology 15:1396-1406(2005) [PubMed: 16096346] [Abstract] Cited for: COMPOSITION OF OLIGOSACCHARYL TRANSFERASE COMPLEXES.
[9]	"Two oligosaccharyl transferase complexes exist in yeast and associate with two different translocons." Yan A., Lennarz W.J. Glycobiology 15:1407-1415(2005) [PubMed: 16096345] [Abstract] Cited for: COMPOSITION OF OLIGOSACCHARYL TRANSFERASE COMPLEXES.
[10]	"Studies of yeast oligosaccharyl transferase subunits using the split-ubiquitin system: topological features and in vivo interactions." Yan A., Wu E., Lennarz W.J. Proc. Natl. Acad. Sci. U.S.A. 102:7121-7126(2005) [PubMed: 15886282] [Abstract] Cited for: TOPOLOGY, INTERACTION WITH OST1; OST2 AND OST4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X97545 Genomic DNA. Translation: CAA66147.1. Z72749 Genomic DNA. Translation: CAA96944.1.
PIR	S71576.
RefSeq	NP_011288.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:2458N.
IntAct	Q92316.
Genome annotation databases
Ensembl	YGL226C-A. Saccharomyces cerevisiae. [Contig view]
GeneID	852625.
GenomeReviews	Gene locus YGL226C-A in contig Y13135_GR.
KEGG	sce:YGL226C-A.
NMPDR	fig|4932.3.peg.2391.
Organism-specific databases
CYGD	YGL226c-a.
SGD	S000003194. OST5.
Phylogenomic databases
HOGENOM	Q92316.
Gene expression databases
GermOnline	YGL226C-A. Saccharomyces cerevisiae.
Family and domain databases
ProDom	Q92316. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
LinkHub	Q92316.
ProtoNet	Search...

Entry information

Entry name

OST5_YEAST

Accession

Primary (citable) accession number: Q92316
Secondary accession number(s): P87030

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 10, 2005
Last modified:	July 22, 2008
This is version 52 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)