Reviewed, UniProtKB/Swiss-Prot Q92688 (AN32B_HUMAN)

Names and origin

Protein names

Recommended name:
    Acidic leucine-rich nuclear phosphoprotein 32 family member B
Alternative name(s):
    Acidic protein rich in leucines
    PHAPI2
    Silver-stainable protein SSP29

Gene names

Name:	ANP32B
Synonyms:	APRIL, PHAPI2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	251 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Multifunctional protein working as a cell cycle progression factor as well as a cell survival factor. Required for the progression from the G1 to the S phase. Anti-apoptotic protein which functions as a caspase-3 inhibitor. Has no phosphatase 2A (PP2A) inhibitor activity By similarity.
Subcellular location	Nucleus. Note= Accumulates in the nuclei at the S phase By similarity.
Tissue specificity	Expressed in heart, lung, pancreas, prostate and in spleen, thymus and placenta.
Sequence similarities	Belongs to the ANP32 family. Contains 4 LRR (leucine-rich) repeats.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Leucine-rich repeat Repeat
PTM	Phosphoprotein
Technical term	3D-structure
Gene Ontology (GO)
None. [Check GOA]

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q92688-1)

Also known as: Anp32b1; PHAPI2b;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q92688-2)

Also known as: Anp32b2; PHAPI2b;

The sequence of this isoform differs from the canonical sequence as follows:
     196-251: Missing.

Notes: No canonical donnor splice site. Cytoplasmic, lacks a nuclear localization signal.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 251	251	Acidic leucine-rich nuclear phosphoprotein 32 family member B
Regions
	Repeat	44 – 62	19	LRR 1
	Repeat	63 – 86	24	LRR 2
	Repeat	87 – 111	25	LRR 3
	Repeat	115 – 138	24	LRR 4
	Compositional bias	146 – 251	106	Asp/Glu-rich (highly acidic)
Amino acid modifications
	Modified residue	244	1	Phosphothreonine
Natural variations
	Alternative sequence	196 – 251	56	Missing in isoform 2.
Experimental info
	Sequence conflict	1 – 2	2	Missing in CAA69265. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Anp32b1) (PHAPI2b) [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 93A1AADF8EDE7D53	FASTA	251	28,788
	10 20 30 40 50 60 MDMKRRIHLE LRNRTPAAVR ELVLDNCKSN DGKIEGLTAE FVNLEFLSLI NVGLISVSNL 70 80 90 100 110 120 PKLPKLKKLE LSENRIFGGL DMLAEKLPNL THLNLSGNKL KDISTLEPLK KLECLKSLDL 130 140 150 160 170 180 FNCEVTNLND YRESVFKLLP QLTYLDGYDR EDQEAPDSDA EVDGVDEEEE DEEGEDEEDE 190 200 210 220 230 240 DDEDGEEEEF DEEDDEDEDV EGDEDDDEVS EEEEEFGLDE EDEDEDEDEE EEEGGKGEKR 250 KRETDDEGED D
	Isoform 2 (Anp32b2) (PHAPI2b) [UniParc]. Checksum: C504A0E604F16F73		195	22,277
	10 20 30 40 50 60 MDMKRRIHLE LRNRTPAAVR ELVLDNCKSN DGKIEGLTAE FVNLEFLSLI NVGLISVSNL 70 80 90 100 110 120 PKLPKLKKLE LSENRIFGGL DMLAEKLPNL THLNLSGNKL KDISTLEPLK KLECLKSLDL 130 140 150 160 170 180 FNCEVTNLND YRESVFKLLP QLTYLDGYDR EDQEAPDSDA EVDGVDEEEE DEEGEDEEDE 190 DDEDGEEEEF DEEDD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Vaesen M., Barnikol-Watanabe S., Kratzin H.D., Hilschmann N. Submitted (AUG-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Molecular cloning and partial characterization of a new silver-stainable protein." Zhu L., Henning D., Valdez B.C. Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]	"Expression analysis and chromosomal mapping of a novel human gene, APRIL, encoding an acidic protein rich in leucines." Mencinger M., Panagopoulos I., Contreras J.A., Mitelman F., Aman P. Biochim. Biophys. Acta 1395:176-180(1998) [PubMed: 9473664] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY. Tissue: Pancreas.
[4]	"DNA sequence and analysis of human chromosome 9." Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I. Nature 429:369-374(2004) [PubMed: 15164053] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Muscle and Testis.
[6]	"The Anp32 family of proteins containing leucine-rich repeats." Matilla A., Radrizzani M. Cerebellum 4:7-18(2005) [PubMed: 15895553] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[7]	"Quantitative phosphoproteome analysis using a dendrimer conjugation chemistry and tandem mass spectrometry." Tao W.A., Wollscheid B., O'Brien R., Eng J.K., Li X.-J., Bodenmiller B., Watts J.D., Hood L., Aebersold R. Nat. Methods 2:591-598(2005) [PubMed: 16094384] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-244, MASS SPECTROMETRY. Tissue: T-cell.
[8]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-244, MASS SPECTROMETRY. Tissue: Epithelium.
[9]	"Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography." Han G., Ye M., Zhou H., Jiang X., Feng S., Jiang X., Tian R., Wan D., Zou H., Gu J. Proteomics 8:1346-1361(2008) [PubMed: 18318008] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-244, MASS SPECTROMETRY. Tissue: Liver.

Cross-references

Sequence databases

EMBLGenBankDDBJ

Y07569 mRNA. Translation: CAA68855.1.
U70439 mRNA. Translation: AAB37579.1.
Y07969 mRNA. Translation: CAA69265.1.
Y07570 mRNA. Translation: CAA68856.1.
AL354726 Genomic DNA. Translation: CAI15260.1.
BC013003 mRNA. Translation: AAH13003.1.
BC019658 mRNA. Translation: AAH19658.1.

RefSeq

NP_006392.1.

UniGene

Hs.494604

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2ELL	NMR	-	A	1-161	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

Q92688.

PTM databases

PhosphoSite

Q92688.

Genome annotation databases

Ensembl

ENSG00000136938. Homo sapiens. [Contig view]

GeneID

10541.

KEGG

hsa:10541.

Organism-specific databases

H-InvDB

HIX0008219.

HGNC

HGNC:16677. ANP32B.

PharmGKB

PA24812.

GenAtlas

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GeneCards

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GeneLynx

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Phylogenomic databases

HOGENOM

Q92688.

HOVERGEN

Q92688.

Gene expression databases

ArrayExpress

Q92688.

CleanEx

HS_ANP32B.

GermOnline

ENSG00000136938. Homo sapiens.

Family and domain databases

InterPro

IPR001611. LRR.
IPR003603. U2A'_phosphoprotein32A_C.
[Graphical view]

Pfam

PF00560. LRR_1. 1 hit.
[Graphical view]

PRINTS

PR00019. LEURICHRPT.

SMART

SM00446. LRRcap. 1 hit.
[Graphical view]

ProDom

Q92688.
[Graphical view] [Entries sharing at least one domain]

BLOCKS

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Other Resources

LinkHub

Q92688.

ProtoNet

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Entry information

Entry name

AN32B_HUMAN

Accession

Primary (citable) accession number: Q92688
Secondary accession number(s): O00655, P78458, P78459

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2002
Last sequence update:	February 1, 1997
Last modified:	July 22, 2008
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)