Reviewed, UniProtKB/Swiss-Prot Q92796 (DLG3_HUMAN)

Names and origin

Protein names

Recommended name:
    Disks large homolog 3
Alternative name(s):
    Synapse-associated protein 102
      Short name=SAP102
    Neuroendocrine-DLG
    XLMR

Gene names

Name:	DLG3
Synonyms:	KIAA1232

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	817 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required for learning most likely through its role in synaptic plasticity following NMDA receptor signaling.
Subunit structure	Interacts through its PDZ domains with GRIN2B and SYNGAP1. Interacts through its guanylate kinase-like domain with DLGAP1, DLGAP2, DLGAP3 and DLGAP4 By similarity. Interacts through its PDZ domains with APC. Interacts through its first two PDZ domains with ERBB4. Interacts through its third PDZ domain with NLGN1, and probably with NLGN2 and NLGN3.
Involvement in disease	Defects in DLG3 are the cause of non-syndromic mental retardation X-linked type 90 (MRX90) [MIM:300189]. Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Non-syndromic mental retardation patients do not manifest other clinical signs.
Sequence similarities	Belongs to the MAGUK family. Contains 1 guanylate kinase-like domain. Contains 3 PDZ (DHR) domains. Contains 1 SH3 domain.

Ontologies

Keywords
Coding sequence diversity	Polymorphism
Disease	Mental retardation
Domain	Repeat SH3 domain
PTM	Phosphoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	negative regulation of cell proliferation Ref.1 Non-traceable author statement. Source: UniProtKB
Cellular component	nucleolus Inferred from direct assay. Source: HPA
Molecular function	guanylate kinase activity Non-traceable author statement. Source: UniProtKB protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ABCA1	O95477	1	EBI-80440,EBI-784112
ANXA1	P04083	1	EBI-80440,EBI-354007
CAMK2A	Q9UQM7	1	EBI-80440,EBI-1383687
CUL2	Q13617	1	EBI-80440,EBI-456179
HIST1H2BC	P62807	1	EBI-80440,EBI-354552
KLHDC3	Q9BQ90	1	EBI-80440,EBI-1055396
KRT31	Q15323	1	EBI-80440,EBI-948001
KRT34	O76011	1	EBI-80440,EBI-1047093
KRT82	Q9NSB4	1	EBI-80440,EBI-1045341
KRT85	P78386	1	EBI-80440,EBI-1049371
S100A3	P33764	1	EBI-80440,EBI-1044747

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 817

817

Disks large homolog 3

PRO_0000094557

Regions

Domain

130 – 217

88

PDZ 1

Domain

226 – 311

86

PDZ 2

Domain

379 – 465

87

PDZ 3

Domain

503 – 568

66

SH3

Domain

627 – 802

176

Guanylate kinase-like

Amino acid modifications

Modified residue

673

1

Phosphotyrosine

Modified residue

808

1

Phosphotyrosine

Natural variations

Natural variant

40

1

G → R in a colorectal cancer sample; somatic mutation.

VAR_036591

Experimental info

Sequence conflict

330 – 381

52

FTALA…EEDFT → AARRERGAMERARKFSGSGL AMGLGSASASAWRRASQRWA WPLRSLRPGGDA Ref.2

Sequence conflict

592 – 606

15

DFPGL…GAKNL → SIKTKRKKSFRLSRKFPFYK SKENMAQESSIQEQGVTSNT SDSESSS Ref.2

Secondary structure

1

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817

Helix Strand Turn

Details...

Beta strand

127 – 135

9

Beta strand

142 – 147

6

Beta strand

160 – 165

6

Helix

170 – 174

5

Beta strand

182 – 186

5

Helix

196 – 205

10

Beta strand

208 – 217

10

Beta strand

224 – 230

7

Beta strand

238 – 242

5

Beta strand

255 – 260

6

Helix

265 – 269

5

Beta strand

277 – 281

5

Helix

291 – 299

9

Beta strand

303 – 310

8

Beta strand

383 – 390

8

Beta strand

396 – 398

3

Beta strand

416 – 418

3

Helix

419 – 422

4

Beta strand

431 – 437

7

Helix

445 – 453

9

Beta strand

457 – 464

8

Sequences

	Sequence		Length	Mass (Da)
	Q92796-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 3D7512EC4713FC4E	FASTA	817	90,344
	10 20 30 40 50 60 MHKHQHCCKC PECYEVTRLA ALRRLEPPGY GDWQVPDPYG PGGGNGASAG YGGYSSQTLP 70 80 90 100 110 120 SQAGATPTPR TKAKLIPTGR DVGPVPLKPV PGKSTPKLNG SGPSWWPECT CTNRDWYEQV 130 140 150 160 170 180 NGSDGMFKYE EIVLERGNSG LGFSIAGGID NPHVPDDPGI FITKIIPGGA AAMDGRLGVN 190 200 210 220 230 240 DCVLRVNEVE VSEVVHSRAV EALKEAGPVV RLVVRRRQPP PETIMEVNLL KGPKGLGFSI 250 260 270 280 290 300 AGGIGNQHIP GDNSIYITKI IEGGAAQKDG RLQIGDRLLA VNNTNLQDVR HEEAVASLKN 310 320 330 340 350 360 TSDMVYLKVA KPGSLHLNDM YAPPDYASTF TALADNHISH NSSLGYLGAV ESKVSYPAPP 370 380 390 400 410 420 QVPPTRYSPI PRHMLAEEDF TREPRKIILH KGSTGLGFNI VGGEDGEGIF VSFILAGGPA 430 440 450 460 470 480 DLSGELRRGD RILSVNGVNL RNATHEQAAA ALKRAGQSVT IVAQYRPEEY SRFESKIHDL 490 500 510 520 530 540 REQMMNSSMS SGSGSLRTSE KRSLYVRALF DYDRTRDSCL PSQGLSFSYG DILHVINASD 550 560 570 580 590 600 DEWWQARLVT PHGESEQIGV IPSKKRVEKK ERARLKTVKF HARTGMIESN RDFPGLSDDY 610 620 630 640 650 660 YGAKNLKGQE DAILSYEPVT RQEIHYARPV IILGPMKDRV NDDLISEFPH KFGSCVPHTT 670 680 690 700 710 720 RPRRDNEVDG QDYHFVVSRE QMEKDIQDNK FIEAGQFNDN LYGTSIQSVR AVAERGKHCI 730 740 750 760 770 780 LDVSGNAIKR LQQAQLYPIA IFIKPKSIEA LMEMNRRQTY EQANKIYDKA MKLEQEFGEY 790 800 810 FTAIVQGDSL EEIYNKIKQI IEDQSGHYIW VPSPEKL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of NE-dlg: a novel human homolog of the Drosophila discs large (dlg) tumor suppressor protein interacts with the APC protein." Makino K., Kuwahara H., Masuko N., Nishiyama Y., Morisaki T., Sasaki J., Nakao M., Kuwano A., Nakata M., Ushio Y., Saya H. Oncogene 14:2425-2433(1997) [PubMed: 9188857] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH APC. Tissue: Fetal brain.
[2]	"Prediction of the coding sequences of unidentified human genes. XV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Ishikawa K., Kikuno R., Hirosawa M., Nomura N., Ohara O. DNA Res. 6:337-345(1999) [PubMed: 10574462] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 330-817. Tissue: Brain.
[3]	"Binding of neuroligins to PSD-95." Irie M., Hata Y., Takeuchi M., Ichtchenko K., Toyoda A., Hirao K., Takai Y., Rosahl T.W., Suedhof T.C. Science 277:1511-1515(1997) [PubMed: 9278515] [Abstract] Cited for: INTERACTION WITH NLGN1; NLGN2 AND NLGN3.
[4]	"The neuregulin receptor ErbB-4 interacts with PDZ-containing proteins at neuronal synapses." Garcia R.A., Vasudevan K., Buonanno A. Proc. Natl. Acad. Sci. U.S.A. 97:3596-3601(2000) [PubMed: 10725395] [Abstract] Cited for: INTERACTION WITH ERBB4.
[5]	"Mutations in the DLG3 gene cause nonsyndromic X-linked mental retardation." Tarpey P., Parnau J., Blow M., Woffendin H., Bignell G., Cox C., Cox J., Davies H., Edkins S., Holden S., Korny A., Mallya U., Moon J., O'Meara S., Parker A., Stephens P., Stevens C., Teague J. , Donnelly A., Mangelsdorf M., Mulley J., Partington M., Turner G., Stevenson R., Schwartz C., Young I., Easton D., Bobrow M., Futreal P.A., Stratton M.R., Gecz J., Wooster R., Raymond F.L. Am. J. Hum. Genet. 75:318-324(2004) [PubMed: 15185169] [Abstract] Cited for: DISEASE.
[6]	"Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer." Rikova K., Guo A., Zeng Q., Possemato A., Yu J., Haack H., Nardone J., Lee K., Reeves C., Li Y., Hu Y., Tan Z., Stokes M., Sullivan L., Mitchell J., Wetzel R., Macneill J., Ren J.M. , Yuan J., Bakalarski C.E., Villen J., Kornhauser J.M., Smith B., Li D., Zhou X., Gygi S.P., Gu T.-L., Polakiewicz R.D., Rush J., Comb M.J. Cell 131:1190-1203(2007) [PubMed: 18083107] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-673 AND TYR-808, MASS SPECTROMETRY.
[7]	"Solution structure of the third PDZ domain of synapse-associated protein 102." RIKEN structural genomics initiative (RSGI) Submitted (MAR-2004) to the PDB data bank Cited for: STRUCTURE BY NMR OF 382-475.
[8]	"The crystal structure of the second PDZ domain of human DLG3." Structural genomics consortium (SGC) Submitted (DEC-2005) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.1 ANGSTROMS) OF 223-314.
[9]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed: 16959974] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] ARG-40.
+	Additional computationally mapped references.

Web resources

GeneReviews

Cross-references

Sequence databases

EMBLGenBankDDBJ

U49089 mRNA. Translation: AAB61453.1.
AB033058 mRNA. Translation: BAA86546.1.

RefSeq

NP_065781.1.

UniGene

Hs.522680

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1UM7	NMR	-	A	382-475	[»]
2FE5	X-ray	1.10	A	223-314	[»]
2I1N	X-ray	1.85	A/B	126-222	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

Q92796.

PTM databases

PhosphoSite

Q92796.

Genome annotation databases

Ensembl

ENSG00000082458. Homo sapiens. [Contig view]

GeneID

1741.

KEGG

hsa:1741.

Organism-specific databases

H-InvDB

HIX0016853.

HGNC

HGNC:2902. DLG3.

HPA

HPA001733.

MIM

300189. gene+phenotype.

Orphanet

98463. Mental retardation, X-linked.

PharmGKB

PA27358.

HUGE

Search...

GenAtlas

Search...

GeneCards

Search...

Phylogenomic databases

HOVERGEN

Q92796.

Gene expression databases

ArrayExpress

Q92796.

CleanEx

HS_DLG3.

GermOnline

ENSG00000082458. Homo sapiens.

Family and domain databases

InterPro

IPR008144. Guanylate_kin.
IPR008145. Guanylt/Ca.
IPR016313. M-assoc_guanylate_kinase.
IPR001478. PDZ.
IPR001452. SH3.
[Graphical view]

Pfam

PF00625. Guanylate_kin. 1 hit.
PF00595. PDZ. 3 hits.
PF00018. SH3_1. 1 hit.
[Graphical view]

PIRSF

PIRSF001741. MAGUK_DLGH. 1 hit.

PRINTS

PR00452. SH3DOMAIN.

ProDom

PD000066. SH3. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00072. GuKc. 1 hit.
SM00228. PDZ. 3 hits.
SM00326. SH3. 1 hit.
[Graphical view]

PROSITE

PS00856. GUANYLATE_KINASE_1. 1 hit.
PS50052. GUANYLATE_KINASE_2. 1 hit.
PS50106. PDZ. 3 hits.
PS50002. SH3. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

7061.

SOURCE

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Entry information

Entry name

DLG3_HUMAN

Accession

Primary (citable) accession number: Q92796
Secondary accession number(s): Q9ULI8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	November 4, 2008
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)