Transcription factor IIIB 90 kDa subunit

Names and origin

Protein names

Recommended name:
    Transcription factor IIIB 90 kDa subunit
      Short name(s)=TFIIIB90, hTFIIIB90
Alternative name(s):
    B-related factor 1
      Short name(s)=BRF-1, hBRF
    TATA box-binding protein-associated factor, RNA polymerase III, subunit 2
    TAF3B2

Gene names

Name:	BRF1
Synonyms:	BRF, GTF3B, TAF3B2, TAF3C

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	677 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	General activator of RNA polymerase which utilizes different TFIIIB complexes at structurally distinct promoters. The isoform 1 is involved in the transcription of tRNA, adenovirus VA1, 7SL and 5S RNA. Isoform 2 is required for transcription of the U6 promoter.
Subunit structure	TFIIIB comprises at least the TATA-binding protein (TBP) and the B-related factor 1 (BRF1/TFIIIB90). Interacts with BDP1.
Subcellular location	NucleusBy similarity.
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR.
Sequence similarities	Belongs to the TFIIB family. Contains 1 TFIIB-type zinc finger.
Sequence caution	AAC50170.1 sequence differs from that shown. Reason: Frameshift at positions 344 and 405.

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Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	rRNA transcription Ref.1 Traceable author statement. Source: ProtInc tRNA transcription Ref.2 Traceable author statement. Source: ProtInc transcription initiation from RNA polymerase III promoter Ref.1 Traceable author statement. Source: ProtInc
Cellular component	transcription factor TFIIIB complex Ref.2 Non-traceable author statement. Source: UniProtKB
Molecular function	RNA polymerase III transcription factor activity Ref.2 Traceable author statement. Source: ProtInc protein binding Ref.1 Traceable author statement. Source: ProtInc transcription factor activity Non-traceable author statement. Source: UniProtKB
Complete GO annotation...

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Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q92994-1) Also known as: hBRF1; This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q92994-2) Also known as: hBRF2; The sequence of this isoform differs from the canonical sequence as follows: 1-204: Missing. 293-338: SYTAGQRKLR...EIELENSRPK → IEEGGQTEAR...CGRGGFTLCP 339-677: Missing.

Isoform 3 (identifier: Q92994-3) Also known as: hBRF3; The sequence of this isoform differs from the canonical sequence as follows: 1-204: Missing.

Isoform 4 (identifier: Q92994-4) Also known as: hBRF4; The sequence of this isoform differs from the canonical sequence as follows: 159-242: NVYVLGKTFL...LLVAARMHDF → DSLRPASFPT...HLPMLGTVGC 243-677: Missing.

Isoform 5 (identifier: Q92994-5) The sequence of this isoform differs from the canonical sequence as follows: 63-89: Missing.

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Molecule processing

Chain

1 – 677

677

Transcription factor IIIB 90 kDa subunit

Regions

Repeat

91 – 172

82

1

Repeat

185 – 269

85

2

Zinc finger

2 – 33

32

TFIIB-type

Sites

Metal binding

6

1

Zinc By similarity

Metal binding

9

1

Zinc By similarity

Metal binding

25

1

Zinc By similarity

Metal binding

28

1

Zinc By similarity

Amino acid modifications

Modified residue

433

1

Phosphoserine

Modified residue

553

1

Phosphoserine

Natural variations

Alternative sequence

1 – 204

204

Missing in isoform 2 and isoform 3.

Alternative sequence

63 – 89

27

Missing in isoform 5.

Alternative sequence

159 – 242

84

NVYVL…RMHDF → DSLRPASFPTWGCDLGVVTR VVTGVYPRCASRISVAGLCC LPSQEVLVCRMRGLHDMGVT VRDLWECGSPWQEGHLPMLG TVGC in isoform 4.

Alternative sequence

243 – 677

435

Missing in isoform 4.

Alternative sequence

293 – 338

46

SYTAG…NSRPK → IEEGGQTEAREPPQASSWEG PSTTRRRSQLWHGCPGCGRG GFTLCP in isoform 2.

Alternative sequence

339 – 677

339

Missing in isoform 2.

Natural variant

542

1

V → M in a colorectal cancer sample; somatic mutation.

Experimental info

Sequence conflict

88

1

N → D in AAC50170. Ref.1

Sequence conflict

151

1

D → V in AAC50170. Ref.1

Sequence conflict

224

1

R → G in AAC50170. Ref.1

Sequence conflict

231

1

A → G in AAC50170. Ref.1

Sequence conflict

339 – 340

2

AK → R in AAC50170. Ref.1

Sequence conflict

594

1

P → T in AAC50170. Ref.1

Sequence conflict

618

1

G → E in AAC50170. Ref.1

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Sequences

Sequence		Length	Mass (Da)
Isoform 1 (hBRF1) [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 5866304B879EC1DE Show »	FASTA	677	73,840
10 20 30 40 50 60 MTGRVCRGCG GTDIELDAAR GDAVCTACGS VLEDNIIVSE VQFVESSGGG SSAVGQFVSL 70 80 90 100 110 120 DGAGKTPTLG GGFHVNLGKE SRAQTLQNGR RHIHHLGNQL QLNQHCLDTA FNFFKMAVSR 130 140 150 160 170 180 HLTRGRKMAH VIAACLYLVC RTEGTPHMLL DLSDLLQVNV YVLGKTFLLL ARELCINAPA 190 200 210 220 230 240 IDPCLYIPRF AHLLEFGEKN HEVSMTALRL LQRMKRDWMH TGRRPSGLCG AALLVAARMH 250 260 270 280 290 300 DFRRTVKEVI SVVKVCESTL RKRLTEFEDT PTSQLTIDEF MKIDLEEECD PPSYTAGQRK 310 320 330 340 350 360 LRMKQLEQVL SKKLEEVEGE ISSYQDAIEI ELENSRPKAK GGLASLAKDG STEDTASSLC 370 380 390 400 410 420 GEEDTEDEEL EAAASHLNKD LYRELLGGAP GSSEAAGSPE WGGRPPALGS LLDPLPTAAS 430 440 450 460 470 480 LGISDSIREC ISSQSSDPKD ASGDGELDLS GIDDLEIDRY ILNESEARVK AELWMRENAE 490 500 510 520 530 540 YLREQREKEA RIAKEKELGI YKEHKPKKSC KRREPIQAST AREAIEKMLE QKKISSKINY 550 560 570 580 590 600 SVLRGLSSAG GGSPHREDAQ PEHSASARKL SRRRTPASRS GADPVTSVGK RLRPLVSTQP 610 620 630 640 650 660 AKKVATGEAL LPSSPTLGAE PARPQAVLVE SGPVSYHADE EADEEEPDEE DGEPCVSALQ 670 MMGSNDYGCD GDEDDGY « Hide
Isoform 2 (hBRF2) [UniParc]. Checksum: 0F56B878F16C484B Show »		134	15,159
10 20 30 40 50 60 MTALRLLQRM KRDWMHTGRR PSGLCGAALL VAARMHDFRR TVKEVISVVK VCESTLRKRL 70 80 90 100 110 120 TEFEDTPTSQ LTIDEFMKID LEEECDPPIE EGGQTEAREP PQASSWEGPS TTRRRSQLWH 130 GCPGCGRGGF TLCP « Hide
Isoform 3 (hBRF3) [UniParc]. Checksum: EF95D8EDB737C5C4 Show »		473	51,798
10 20 30 40 50 60 MTALRLLQRM KRDWMHTGRR PSGLCGAALL VAARMHDFRR TVKEVISVVK VCESTLRKRL 70 80 90 100 110 120 TEFEDTPTSQ LTIDEFMKID LEEECDPPSY TAGQRKLRMK QLEQVLSKKL EEVEGEISSY 130 140 150 160 170 180 QDAIEIELEN SRPKAKGGLA SLAKDGSTED TASSLCGEED TEDEELEAAA SHLNKDLYRE 190 200 210 220 230 240 LLGGAPGSSE AAGSPEWGGR PPALGSLLDP LPTAASLGIS DSIRECISSQ SSDPKDASGD 250 260 270 280 290 300 GELDLSGIDD LEIDRYILNE SEARVKAELW MRENAEYLRE QREKEARIAK EKELGIYKEH 310 320 330 340 350 360 KPKKSCKRRE PIQASTAREA IEKMLEQKKI SSKINYSVLR GLSSAGGGSP HREDAQPEHS 370 380 390 400 410 420 ASARKLSRRR TPASRSGADP VTSVGKRLRP LVSTQPAKKV ATGEALLPSS PTLGAEPARP 430 440 450 460 470 QAVLVESGPV SYHADEEADE EEPDEEDGEP CVSALQMMGS NDYGCDGDED DGY « Hide
Isoform 4 (hBRF4) [UniParc]. Checksum: B6FC691408BA8E26 Show »		242	25,922
10 20 30 40 50 60 MTGRVCRGCG GTDIELDAAR GDAVCTACGS VLEDNIIVSE VQFVESSGGG SSAVGQFVSL 70 80 90 100 110 120 DGAGKTPTLG GGFHVNLGKE SRAQTLQNGR RHIHHLGNQL QLNQHCLDTA FNFFKMAVSR 130 140 150 160 170 180 HLTRGRKMAH VIAACLYLVC RTEGTPHMLL DLSDLLQVDS LRPASFPTWG CDLGVVTRVV 190 200 210 220 230 240 TGVYPRCASR ISVAGLCCLP SQEVLVCRMR GLHDMGVTVR DLWECGSPWQ EGHLPMLGTV GC « Hide
Isoform 5 [UniParc]. Checksum: EC6958C644660237 Show »		650	71,119
10 20 30 40 50 60 MTGRVCRGCG GTDIELDAAR GDAVCTACGS VLEDNIIVSE VQFVESSGGG SSAVGQFVSL 70 80 90 100 110 120 DGRRHIHHLG NQLQLNQHCL DTAFNFFKMA VSRHLTRGRK MAHVIAACLY LVCRTEGTPH 130 140 150 160 170 180 MLLDLSDLLQ VNVYVLGKTF LLLARELCIN APAIDPCLYI PRFAHLLEFG EKNHEVSMTA 190 200 210 220 230 240 LRLLQRMKRD WMHTGRRPSG LCGAALLVAA RMHDFRRTVK EVISVVKVCE STLRKRLTEF 250 260 270 280 290 300 EDTPTSQLTI DEFMKIDLEE ECDPPSYTAG QRKLRMKQLE QVLSKKLEEV EGEISSYQDA 310 320 330 340 350 360 IEIELENSRP KAKGGLASLA KDGSTEDTAS SLCGEEDTED EELEAAASHL NKDLYRELLG 370 380 390 400 410 420 GAPGSSEAAG SPEWGGRPPA LGSLLDPLPT AASLGISDSI RECISSQSSD PKDASGDGEL 430 440 450 460 470 480 DLSGIDDLEI DRYILNESEA RVKAELWMRE NAEYLREQRE KEARIAKEKE LGIYKEHKPK 490 500 510 520 530 540 KSCKRREPIQ ASTAREAIEK MLEQKKISSK INYSVLRGLS SAGGGSPHRE DAQPEHSASA 550 560 570 580 590 600 RKLSRRRTPA SRSGADPVTS VGKRLRPLVS TQPAKKVATG EALLPSSPTL GAEPARPQAV 610 620 630 640 650 LVESGPVSYH ADEEADEEEP DEEDGEPCVS ALQMMGSNDY GCDGDEDDGY « Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and function of a human transcription factor TFIIIB subunit that is evolutionarily conserved and contains both TFIIB- and high-mobility-group protein 2-related domains." Wang Z., Roeder R.G. Proc. Natl. Acad. Sci. U.S.A. 92:7026-7030(1995) [PubMed: 7624363] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PROTEIN SEQUENCE OF 264-281; 314-338; 441-469 AND 471-488.
[2]	"RNA polymerase III transcription from the human U6 and adenovirus type 2 VAI promoters has different requirements for human BRF, a subunit of human TFIIIB." Mital R., Kobayashi R., Hernandez N. Mol. Cell. Biol. 16:7031-7042(1996) [PubMed: 8943358] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PROTEIN SEQUENCE OF 248-254; 263-280; 313-320; 343-348; 380-396; 440-449; 471-488; 514-527; 538-561 AND 570-602.
[3]	"Alternatively spliced hBRF variants function at different RNA polymerase III promoters." McCulloch V., Hardin P., Peng W., Ruppert J.M., Lobo-Ruppert S.M. EMBO J. 19:4134-4143(2000) [PubMed: 10921893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2; 3 AND 4).
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 5). Tissue: Brain and Skin.
[5]	"TFIIIB is phosphorylated, disrupted and selectively released from tRNA promoters during mitosis in vivo." Fairley J.A., Scott P.H., White R.J. EMBO J. 22:5841-5850(2003) [PubMed: 14592981] [Abstract] Cited for: INTERACTION WITH BDP1.
[6]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-553, MASS SPECTROMETRY. Tissue: Epithelium.
[7]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-433, MASS SPECTROMETRY.
[8]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. Velculescu V.E. Science 314:268-274(2006) [PubMed: 16959974] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] MET-542.

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Cross-references

Sequence databases
	U28838 mRNA. Translation: AAC50170.1. Frameshift. U75276 mRNA. Translation: AAB38876.1. AJ297406 mRNA. Translation: CAC04512.1. AJ297407 mRNA. Translation: CAC04513.1. AJ297408 mRNA. Translation: CAC04514.1. BC071637 mRNA. Translation: AAH71637.1. BC086856 mRNA. Translation: AAH86856.1.
PIR	I39065.
RefSeq	NP_001510.2. NP_663718.1.
UniGene	Hs.424484
3D structure databases
HSSP	HSSP built from PDB template 1AIS based on UniProtKB P29095.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q92994.
PTM databases
PhosphoSite	Q92994.
Genome annotation databases
Ensembl	ENSG00000185024. Homo sapiens. [Contig view]
GeneID	2972.
KEGG	hsa:2972.
NMPDR	fig\|9606.3.peg.10278.
Organism-specific databases
H-InvDB	HIX0012028.
HGNC	HGNC:11551. BRF1.
MIM	604902. gene.
PharmGKB	PA25420.
GenAtlas	Search...
GeneCards	Search...
GeneLynx	Search...
Phylogenomic databases
HOVERGEN	Q92994.
Enzyme and pathway databases
Reactome	REACT_1788. Transcription.
Gene expression databases
ArrayExpress	Q92994.
CleanEx	HS_BRF1.
GermOnline	ENSG00000185024. Homo sapiens.
Family and domain databases
InterPro	IPR011665. BRF1_TBP_bd. IPR006670. Cyclin. IPR013763. Cyclin_related. IPR013150. TFIIB_cyclin. IPR000812. TFIIB_euk_relate. IPR013137. Znf_TFIIB. [Graphical view]
Gene3D	G3DSA:1.10.472.10. Cyclin_related. 2 hits.
PANTHER	PTHR11618. TFIIB_euk_relate. 1 hit.
Pfam	PF07741. BRF1. 1 hit. PF00382. TFIIB. 2 hits. PF08271. TFIIB_Zn_Ribbon. 1 hit. [Graphical view]
PRINTS	PR00685. TIFACTORIIB.
SMART	SM00385. CYCLIN. 2 hits. [Graphical view]
PROSITE	PS00782. TFIIB. False negative. PS51134. ZF_TFIIB. 1 hit. [Graphical view]
ProDom	Q92994. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
SOURCE	Search...
ProtoNet	Search...

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Entry information

Entry name

TF3B_HUMAN

Accession

Primary (citable) accession number: Q92994
Secondary accession number(s): Q13223

Q9HCW8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2002
Last sequence update:	February 1, 1997
Last modified:	July 22, 2008
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Human chromosome 14: entries, gene names and cross-references to MIM

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List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

UniProtKB secondary accession numbers

Index of UniProtKB secondary accession numbers

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Keywords

Gene Ontology (GO)

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents