Reviewed, UniProtKB/Swiss-Prot Q93070 (NAR4_HUMAN)

Names and origin

Protein names

Recommended name:
    Ecto-ADP-ribosyltransferase 4
    EC=2.4.2.31
Alternative name(s):
    NAD(P)(+)--arginine ADP-ribosyltransferase 4
    Mono(ADP-ribosyl)transferase 4
    Dombrock blood group carrier molecule
    CD_antigen=CD297

Gene names

Name:	ART4
Synonyms:	DO, DOK1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	NAD(+) + protein-L-arginine = nicotinamide + N(omega)-(ADP-D-ribosyl)-protein-L-arginine. NADP(+) + protein-L-arginine = nicotinamide + N(omega)-((2'-phospho-ADP)-D-ribosyl)-protein-L-arginine.
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor.
Tissue specificity	Expressed in spleen and T-cells.
Polymorphism	DO is responsible for the Dombrock blood group system. The molecular basis of the Do(a)/Do(b) blood group antigen is a single variation in position 265; Asn-265 corresponds to Do(a) and Asp-265 to Do(b). It is also responsible for the antigens Gregory [Gy(a)], Holley [Hy] and Joseph [Jo(a)].
Sequence similarities	Belongs to the Arg-specific ADP-ribosyltransferase family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Signal
Ligand	NAD NADP
Molecular function	Blood group antigen Glycosyltransferase Transferase
PTM	GPI-anchor Glycoprotein Lipoprotein
Gene Ontology (GO)
Biological process	arginine metabolic process Ref.1 Non-traceable author statement. Source: UniProtKB protein amino acid ADP-ribosylation Ref.5 Non-traceable author statement. Source: UniProtKB
Cellular component	membrane Ref.5 Non-traceable author statement. Source: UniProtKB
Molecular function	NAD(P)+-protein-arginine ADP-ribosyltransferase activity Ref.5 Non-traceable author statement. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Signal peptide	1 – 46	46	Potential
	Chain	47 – 285	239	Ecto-ADP-ribosyltransferase 4
	Propeptide	286 – 314	29	Removed in mature form Potential
Sites
	Active site	251	1	By similarity
Amino acid modifications
	Lipidation	285	1	GPI-anchor amidated alanine Potential
	Glycosylation	114	1	N-linked (GlcNAc...) Potential
	Glycosylation	178	1	N-linked (GlcNAc...) Potential
	Glycosylation	222	1	N-linked (GlcNAc...) Potential
	Glycosylation	257	1	N-linked (GlcNAc...) Potential
	Glycosylation	274	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	108	1	G → V in Hy1 and Hy2.
	Natural variant	117	1	T → I in Jo(a).
	Natural variant	135	1	D → E
	Natural variant	189	1	T → M
	Natural variant	265	1	N → D in Do(b), Hy1 and Hy2. dbSNP rs11276.
	Natural variant	300	1	L → V in Hy1.
Experimental info
	Sequence conflict	48	1	E → Q in CAA65095. Ref.5
	Sequence conflict	116	1	T → S in CAA65095. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q93070-1 [UniParc]. Last modified August 13, 2002. Version 2. Checksum: 174913890C9D158F	FASTA	314	35,878
	10 20 30 40 50 60 MGPLINRCKK ILLPTTVPPA TMRIWLLGGL LPFLLLLSGL QRPTEGSEVA IKIDFDFAPG 70 80 90 100 110 120 SFDDQYQGCS KQVMEKLTQG DYFTKDIEAQ KNYFRMWQKA HLAWLNQGKV LPQNMTTTHA 130 140 150 160 170 180 VAILFYTLNS NVHSDFTRAM ASVARTPQQY ERSFHFKYLH YYLTSAIQLL RKDSIMENGT 190 200 210 220 230 240 LCYEVHYRTK DVHFNAYTGA TIRFGQFLST SLLKEEAQEF GNQTLFTIFT CLGAPVQYFS 250 260 270 280 290 300 LKKEVLIPPY ELFKVINMSY HPRGNWLQLR STGNLSTYNC QLLKASSKKC IPDPIAIASL 310 SFLTSVIIFS KSRV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of the Dombrock blood group glycoprotein as a polymorphic member of the ADP-ribosyltransferase gene family." Gubin A.N., Njoroge J.M., Wojda U., Pack S.D., Rios M., Reid M.E., Miller J.L. Blood 96:2621-2627(2000) [PubMed: 11001920] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT DO(B).
[2]	"Insights into the Holley- and Joseph- phenotypes." Rios M., Hue-Roye K., Oyen R., Miller J., Reid M.E. Transfusion 42:52-58(2002) [PubMed: 11896313] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS DO(B); JO(A); HY1 AND HY2.
[3]	SeattleSNPs program for genomic applications Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS VAL-108; ILE-117; GLU-135; MET-189; ASP-265 AND VAL-300.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[5]	"Two novel human members of an emerging mammalian gene family related to mono-ADP-ribosylating bacterial toxins." Koch-Nolte F., Haag F., Braren R., Kuehl M., Hoovers J., Balasubramanian S., Bazan J.F., Thiele H.-G. Genomics 39:370-376(1997) [PubMed: 9119374] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 48-314.
[6]	"Polymerase chain reaction with sequence-specific primers-based genotyping of the human Dombrock blood group DO1 and DO2 alleles and the DO gene frequencies in Chinese blood donors." Wu G.-G., Jin S.-Z., Deng Z.-H., Zhao T.-M. Vox Sang. 81:49-51(2001) [PubMed: 11520417] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 49-284, VARIANT DO(B).

Web resources

BGMUT

Blood group antigen gene mutation database"

SeattleSNPs

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF290204 mRNA. Translation: AAG17845.1. AF382213, AF382211, AF382212 Genomic DNA. Translation: AAM21462.1. AF382216, AF382214, AF382215 Genomic DNA. Translation: AAM21464.1. AF382219, AF382217, AF382218 Genomic DNA. Translation: AAM21465.1. AF382222, AF382220, AF382221 Genomic DNA. Translation: AAM21466.1. AF382225, AF382223, AF382224 Genomic DNA. Translation: AAM21467.1. AY899803 Genomic DNA. Translation: AAW65375.1. BC074727 mRNA. Translation: AAH74727.1. X95826 Genomic DNA. Translation: CAA65095.1. AF340233 Genomic DNA. Translation: AAK11274.1. AF340234 Genomic DNA. Translation: AAK11275.1.
RefSeq	NP_066549.2.
UniGene	Hs.591158 Hs.655792 Hs.668803
3D structure databases
HSSP	HSSP built from PDB template 1GXY based on UniProtKB P20974.
ModBase	Search...
Genome annotation databases
Ensembl	ENSG00000111339. Homo sapiens. [Contig view]
GeneID	420.
KEGG	hsa:420.
Organism-specific databases
H-InvDB	HIX0036719.
HGNC	HGNC:726. ART4.
MIM	110600. gene+phenotype.
PharmGKB	PA142672580.
GenAtlas	Search...
GeneCards	Search...
GeneLynx	Search...
Phylogenomic databases
HOGENOM	Q93070.
HOVERGEN	Q93070.
Gene expression databases
ArrayExpress	Q93070.
CleanEx	HS_ART4. HS_DOK1.
GermOnline	ENSG00000111339. Homo sapiens.
Family and domain databases
InterPro	IPR000768. ART. [Graphical view]
PANTHER	PTHR10339. ART. 1 hit.
Pfam	PF01129. ART. 1 hit. [Graphical view]
PRINTS	PR00970. RIBTRNSFRASE.
PROSITE	PS01291. ART. 1 hit. [Graphical view]
ProDom	Q93070. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
SOURCE	Search...
ProtoNet	Search...

Entry information

Entry name

NAR4_HUMAN

Accession

Primary (citable) accession number: Q93070
Secondary accession number(s): Q9BZ50, Q9BZ51, Q9HB06

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	August 13, 2002
Last modified:	July 22, 2008
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)