Reviewed, UniProtKB/Swiss-Prot Q94361 (GEI17_CAEEL)

Names and origin

Protein names

Recommended name:
    E3 SUMO-protein ligase gei-17
Alternative name(s):
    Gex-3-interacting protein 17

Gene names

Name:	gei-17
ORF Names:	W10D5.3

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	790 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Functions as an E3-type smo-1 ligase. In the early embryo, specifically suppresses checkpoint activation in response to DNA damage, maybe by promoting mus-101 sumoylation.
Pathway	Protein modification; protein sumoylation.
Subunit structure	May interact with gex-3.
Sequence similarities	Belongs to the PIAS family. Contains 1 SP-RING-type zinc finger.

Ontologies

Keywords
Biological process	DNA damage DNA repair Ubl conjugation pathway
Coding sequence diversity	Alternative splicing
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	body morphogenesis Inferred from mutant phenotype. Source: WormBase embryonic development ending in birth or egg hatching Ref.2 Inferred from mutant phenotype. Source: WormBase hermaphrodite genitalia development Inferred from mutant phenotype. Source: WormBase positive regulation of growth rate Inferred from mutant phenotype. Source: WormBase positive regulation of multicellular organism growth Inferred from mutant phenotype. Source: WormBase
Complete GO annotation...

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q94361-1)

Also known as: c;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Notes: No experimental confirmation available.

Isoform 2 (identifier: Q94361-2)

Also known as: f;

The sequence of this isoform differs from the canonical sequence as follows:
     162-216: Missing.
     773-790: GAFAYYPPQYPQQQYRQN → DTNNIQPPRILSMAEIQANASRLLVNGHLMIDHSGVTMRNPRGSRN

Notes: No experimental confirmation available.

Isoform 3 (identifier: Q94361-3)

Also known as: g;

The sequence of this isoform differs from the canonical sequence as follows:
     95-216: Missing.
     671-671: Missing.
     773-790: GAFAYYPPQYPQQQYRQN → DTNNIQPPRILSMAEIQANASRLLVNGHLMIDHSGVTMRNPRGSRN

Notes: No experimental confirmation available.

Isoform 4 (identifier: Q94361-4)

Also known as: e;

The sequence of this isoform differs from the canonical sequence as follows:
     162-216: Missing.
     569-584: Missing.
     671-716: Missing.

Notes: No experimental confirmation available.

Isoform 5 (identifier: Q94361-5)

Also known as: a;

The sequence of this isoform differs from the canonical sequence as follows:
     162-216: Missing.
     671-716: Missing.

Notes: No experimental confirmation available.

Isoform 6 (identifier: Q94361-6)

Also known as: d;

The sequence of this isoform differs from the canonical sequence as follows:
     95-96: Missing.
     162-216: Missing.
     671-716: Missing.

Notes: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 790	790	E3 SUMO-protein ligase gei-17
Regions
	Zinc finger	410 – 487	78	SP-RING-type
	Compositional bias	128 – 156	29	Gln-rich
	Compositional bias	182 – 188	7	Poly-Ala
	Compositional bias	516 – 521	6	Poly-Asp
	Compositional bias	560 – 563	4	Poly-Asp
	Compositional bias	721 – 789	69	Gln-rich
Natural variations
	Alternative sequence	95 – 216	122	Missing in isoform 3.
	Alternative sequence	95 – 96	2	Missing in isoform 6.
	Alternative sequence	162 – 216	55	Missing in isoform 2, isoform 4, isoform 5 and isoform 6.
	Alternative sequence	569 – 584	16	Missing in isoform 4.
	Alternative sequence	671 – 716	46	Missing in isoform 4, isoform 5 and isoform 6.
	Alternative sequence	671	1	Missing in isoform 3.
	Alternative sequence	773 – 790	18	GAFAY…QYRQN → DTNNIQPPRILSMAEIQANA SRLLVNGHLMIDHSGVTMRN PRGSRN in isoform 2 and isoform 3.

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (c) [UniParc]. Last modified October 1, 2003. Version 2. Checksum: 78EC9A033B160EF4	FASTA	790	89,227
	10 20 30 40 50 60 MLPNNQWQIP INNGLTHQEN MAAHAAVMKL RVHDLQSIIS QLSLRKPRPQ KSEHQKVVVE 70 80 90 100 110 120 SLRDPHHARQ IYQMVISLIF SSLQASNFPN GNYEMQKRPA TTSQVRSHPY VLPSRSGASN 130 140 150 160 170 180 HLVNHHYQQQ QQQQPQPHNL LHQQMMASHH SHLQQQHHPS TVRWLTPELL EEQLRGSMRY 190 200 210 220 230 240 GAPAAAAATN APLHSSFPNH GRSSQQSLQK SEKSNRPKKM YADNFEPLPL PFYDVISVLL 250 260 270 280 290 300 KPVELHSSDS PTLKQTKQLQ FPFLLTAEHI SKISYRADVT PLPRYELQLR FFNLTEPVQG 310 320 330 340 350 360 PQKDDFPLNC YARVDDSVVQ LPNVIPTNKT NAEPKRPSRP VNITSNMNRY KKEHTVAVEW 370 380 390 400 410 420 LADKRVWAAG VYFVHRVNSD ILFKRLNQNV SRHRSLEVTK QEVIKKLSGG EDDIAMDRLN 430 440 450 460 470 480 ISLLDPLCKT RMTTPSRCQD CTHLQCFDLL SYLMMNEKKP TWQCPVCSSN CPYDRLIVDD 490 500 510 520 530 540 YFLDMLAKVD KNTTEVELKE DGSYDVIKEE AFCISDDDDD DVVPATVNGT ASCSSTNGNG 550 560 570 580 590 600 LANEAAKKKP ADDDIITLSD DDDEELNRGI MNSLNDSFSP GRHTASAELA AQKTPPQQKK 610 620 630 640 650 660 KTKDDDIEII TLDDTPPRPV AASANLPMRQ MSQQNQMPVG SSPSGMASTQ MGMNEGASKT 670 680 690 700 710 720 IRDALNKIGE QSANSSTQSS PLVQLHHTTH PLNFAQSSYM NPSSGSQTPT SQYGYSPMIN 730 740 750 760 770 780 QAPHFQMQNG LIGRNNQMVH MQQHHLQQQQ QQQQSPQIMS PSFYAQQQMS NGGAFAYYPP 790 QYPQQQYRQN
	Isoform 2 (f) [UniParc]. Checksum: 49DF14DDDD87AC80		763	86,060
	10 20 30 40 50 60 MLPNNQWQIP INNGLTHQEN MAAHAAVMKL RVHDLQSIIS QLSLRKPRPQ KSEHQKVVVE 70 80 90 100 110 120 SLRDPHHARQ IYQMVISLIF SSLQASNFPN GNYEMQKRPA TTSQVRSHPY VLPSRSGASN 130 140 150 160 170 180 HLVNHHYQQQ QQQQPQPHNL LHQQMMASHH SHLQQQHHPS TPKKMYADNF EPLPLPFYDV 190 200 210 220 230 240 ISVLLKPVEL HSSDSPTLKQ TKQLQFPFLL TAEHISKISY RADVTPLPRY ELQLRFFNLT 250 260 270 280 290 300 EPVQGPQKDD FPLNCYARVD DSVVQLPNVI PTNKTNAEPK RPSRPVNITS NMNRYKKEHT 310 320 330 340 350 360 VAVEWLADKR VWAAGVYFVH RVNSDILFKR LNQNVSRHRS LEVTKQEVIK KLSGGEDDIA 370 380 390 400 410 420 MDRLNISLLD PLCKTRMTTP SRCQDCTHLQ CFDLLSYLMM NEKKPTWQCP VCSSNCPYDR 430 440 450 460 470 480 LIVDDYFLDM LAKVDKNTTE VELKEDGSYD VIKEEAFCIS DDDDDDVVPA TVNGTASCSS 490 500 510 520 530 540 TNGNGLANEA AKKKPADDDI ITLSDDDDEE LNRGIMNSLN DSFSPGRHTA SAELAAQKTP 550 560 570 580 590 600 PQQKKKTKDD DIEIITLDDT PPRPVAASAN LPMRQMSQQN QMPVGSSPSG MASTQMGMNE 610 620 630 640 650 660 GASKTIRDAL NKIGEQSANS STQSSPLVQL HHTTHPLNFA QSSYMNPSSG SQTPTSQYGY 670 680 690 700 710 720 SPMINQAPHF QMQNGLIGRN NQMVHMQQHH LQQQQQQQQS PQIMSPSFYA QQQMSNGDTN 730 740 750 760 NIQPPRILSM AEIQANASRL LVNGHLMIDH SGVTMRNPRG SRN
	Isoform 3 (g) [UniParc]. Checksum: 54CF3A0BA9E1CB74		695	78,126
	10 20 30 40 50 60 MLPNNQWQIP INNGLTHQEN MAAHAAVMKL RVHDLQSIIS QLSLRKPRPQ KSEHQKVVVE 70 80 90 100 110 120 SLRDPHHARQ IYQMVISLIF SSLQASNFPN GNYEPKKMYA DNFEPLPLPF YDVISVLLKP 130 140 150 160 170 180 VELHSSDSPT LKQTKQLQFP FLLTAEHISK ISYRADVTPL PRYELQLRFF NLTEPVQGPQ 190 200 210 220 230 240 KDDFPLNCYA RVDDSVVQLP NVIPTNKTNA EPKRPSRPVN ITSNMNRYKK EHTVAVEWLA 250 260 270 280 290 300 DKRVWAAGVY FVHRVNSDIL FKRLNQNVSR HRSLEVTKQE VIKKLSGGED DIAMDRLNIS 310 320 330 340 350 360 LLDPLCKTRM TTPSRCQDCT HLQCFDLLSY LMMNEKKPTW QCPVCSSNCP YDRLIVDDYF 370 380 390 400 410 420 LDMLAKVDKN TTEVELKEDG SYDVIKEEAF CISDDDDDDV VPATVNGTAS CSSTNGNGLA 430 440 450 460 470 480 NEAAKKKPAD DDIITLSDDD DEELNRGIMN SLNDSFSPGR HTASAELAAQ KTPPQQKKKT 490 500 510 520 530 540 KDDDIEIITL DDTPPRPVAA SANLPMRQMS QQNQMPVGSS PSGMASTQMG MNEGASKTIR 550 560 570 580 590 600 DALNKIGESA NSSTQSSPLV QLHHTTHPLN FAQSSYMNPS SGSQTPTSQY GYSPMINQAP 610 620 630 640 650 660 HFQMQNGLIG RNNQMVHMQQ HHLQQQQQQQ QSPQIMSPSF YAQQQMSNGD TNNIQPPRIL 670 680 690 SMAEIQANAS RLLVNGHLMI DHSGVTMRNP RGSRN
	Isoform 4 (e) [UniParc]. Checksum: 7DBA125BC7712F7C		673	76,543
	10 20 30 40 50 60 MLPNNQWQIP INNGLTHQEN MAAHAAVMKL RVHDLQSIIS QLSLRKPRPQ KSEHQKVVVE 70 80 90 100 110 120 SLRDPHHARQ IYQMVISLIF SSLQASNFPN GNYEMQKRPA TTSQVRSHPY VLPSRSGASN 130 140 150 160 170 180 HLVNHHYQQQ QQQQPQPHNL LHQQMMASHH SHLQQQHHPS TPKKMYADNF EPLPLPFYDV 190 200 210 220 230 240 ISVLLKPVEL HSSDSPTLKQ TKQLQFPFLL TAEHISKISY RADVTPLPRY ELQLRFFNLT 250 260 270 280 290 300 EPVQGPQKDD FPLNCYARVD DSVVQLPNVI PTNKTNAEPK RPSRPVNITS NMNRYKKEHT 310 320 330 340 350 360 VAVEWLADKR VWAAGVYFVH RVNSDILFKR LNQNVSRHRS LEVTKQEVIK KLSGGEDDIA 370 380 390 400 410 420 MDRLNISLLD PLCKTRMTTP SRCQDCTHLQ CFDLLSYLMM NEKKPTWQCP VCSSNCPYDR 430 440 450 460 470 480 LIVDDYFLDM LAKVDKNTTE VELKEDGSYD VIKEEAFCIS DDDDDDVVPA TVNGTASCSS 490 500 510 520 530 540 TNGNGLANEA AKKKPADDDI ITLSDDDDEE LNRASAELAA QKTPPQQKKK TKDDDIEIIT 550 560 570 580 590 600 LDDTPPRPVA ASANLPMRQM SQQNQMPVGS SPSGMASTQM GMNEGASKTI RDALNKIGEP 610 620 630 640 650 660 MINQAPHFQM QNGLIGRNNQ MVHMQQHHLQ QQQQQQQSPQ IMSPSFYAQQ QMSNGGAFAY 670 YPPQYPQQQY RQN
	Isoform 5 (a) [UniParc]. Checksum: 57A205414D9A82D8		689	78,258
	10 20 30 40 50 60 MLPNNQWQIP INNGLTHQEN MAAHAAVMKL RVHDLQSIIS QLSLRKPRPQ KSEHQKVVVE 70 80 90 100 110 120 SLRDPHHARQ IYQMVISLIF SSLQASNFPN GNYEMQKRPA TTSQVRSHPY VLPSRSGASN 130 140 150 160 170 180 HLVNHHYQQQ QQQQPQPHNL LHQQMMASHH SHLQQQHHPS TPKKMYADNF EPLPLPFYDV 190 200 210 220 230 240 ISVLLKPVEL HSSDSPTLKQ TKQLQFPFLL TAEHISKISY RADVTPLPRY ELQLRFFNLT 250 260 270 280 290 300 EPVQGPQKDD FPLNCYARVD DSVVQLPNVI PTNKTNAEPK RPSRPVNITS NMNRYKKEHT 310 320 330 340 350 360 VAVEWLADKR VWAAGVYFVH RVNSDILFKR LNQNVSRHRS LEVTKQEVIK KLSGGEDDIA 370 380 390 400 410 420 MDRLNISLLD PLCKTRMTTP SRCQDCTHLQ CFDLLSYLMM NEKKPTWQCP VCSSNCPYDR 430 440 450 460 470 480 LIVDDYFLDM LAKVDKNTTE VELKEDGSYD VIKEEAFCIS DDDDDDVVPA TVNGTASCSS 490 500 510 520 530 540 TNGNGLANEA AKKKPADDDI ITLSDDDDEE LNRGIMNSLN DSFSPGRHTA SAELAAQKTP 550 560 570 580 590 600 PQQKKKTKDD DIEIITLDDT PPRPVAASAN LPMRQMSQQN QMPVGSSPSG MASTQMGMNE 610 620 630 640 650 660 GASKTIRDAL NKIGEPMINQ APHFQMQNGL IGRNNQMVHM QQHHLQQQQQ QQQSPQIMSP 670 680 SFYAQQQMSN GGAFAYYPPQ YPQQQYRQN
	Isoform 6 (d) [UniParc]. Checksum: F3C969C0E5CEB230		687	77,999
	10 20 30 40 50 60 MLPNNQWQIP INNGLTHQEN MAAHAAVMKL RVHDLQSIIS QLSLRKPRPQ KSEHQKVVVE 70 80 90 100 110 120 SLRDPHHARQ IYQMVISLIF SSLQASNFPN GNYEKRPATT SQVRSHPYVL PSRSGASNHL 130 140 150 160 170 180 VNHHYQQQQQ QQPQPHNLLH QQMMASHHSH LQQQHHPSTP KKMYADNFEP LPLPFYDVIS 190 200 210 220 230 240 VLLKPVELHS SDSPTLKQTK QLQFPFLLTA EHISKISYRA DVTPLPRYEL QLRFFNLTEP 250 260 270 280 290 300 VQGPQKDDFP LNCYARVDDS VVQLPNVIPT NKTNAEPKRP SRPVNITSNM NRYKKEHTVA 310 320 330 340 350 360 VEWLADKRVW AAGVYFVHRV NSDILFKRLN QNVSRHRSLE VTKQEVIKKL SGGEDDIAMD 370 380 390 400 410 420 RLNISLLDPL CKTRMTTPSR CQDCTHLQCF DLLSYLMMNE KKPTWQCPVC SSNCPYDRLI 430 440 450 460 470 480 VDDYFLDMLA KVDKNTTEVE LKEDGSYDVI KEEAFCISDD DDDDVVPATV NGTASCSSTN 490 500 510 520 530 540 GNGLANEAAK KKPADDDIIT LSDDDDEELN RGIMNSLNDS FSPGRHTASA ELAAQKTPPQ 550 560 570 580 590 600 QKKKTKDDDI EIITLDDTPP RPVAASANLP MRQMSQQNQM PVGSSPSGMA STQMGMNEGA 610 620 630 640 650 660 SKTIRDALNK IGEPMINQAP HFQMQNGLIG RNNQMVHMQQ HHLQQQQQQQ QSPQIMSPSF 670 680 YAQQQMSNGG AFAYYPPQYP QQQYRQN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2.
[2]	"Isolation of the interacting molecules with GEX-3 by a novel functional screening." Tsuboi D., Qadota H., Kasuya K., Amano M., Kaibuchi K. Biochem. Biophys. Res. Commun. 292:697-701(2002) [PubMed: 11922622] [Abstract] Cited for: POSSIBLE INTERACTION WITH GEX-3.
[3]	"Systematic, RNA-interference-mediated identification of mus-101 modifier genes in Caenorhabditis elegans." Holway A.H., Hung C., Michael W.M. Genetics 169:1451-1460(2005) [PubMed: 15654100] [Abstract] Cited for: FUNCTION.
[4]	"The T-box factor TBX-2 and the SUMO conjugating enzyme UBC-9 are required for ABa-derived pharyngeal muscle in C. elegans." Roy Chowdhuri S., Crum T., Woollard A., Aslam S., Okkema P.G. Dev. Biol. 295:664-677(2006) [PubMed: 16701625] [Abstract] Cited for: FUNCTION.
[5]	"Checkpoint silencing during the DNA damage response in Caenorhabditis elegans embryos." Holway A.H., Kim S.-H., La Volpe A., Michael W.M. J. Cell Biol. 172:999-1008(2006) [PubMed: 16549501] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z79758 Genomic DNA. Translation: CAB02133.2. Z79758 Genomic DNA. Translation: CAB02134.1. Z79758 Genomic DNA. Translation: CAB54321.1. Z79758 Genomic DNA. Translation: CAD98729.1. Z79758 Genomic DNA. Translation: CAD98730.1. Z79758 Genomic DNA. Translation: CAI79179.1.
PIR	T26330. T26331. T26332.
RefSeq	NP_492444.2.
UniGene	Cel.5457
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	W10D5.3. Caenorhabditis elegans. [Contig view]
GeneID	172733.
NMPDR	fig|6239.3.peg.2408.
Organism-specific databases
WormBase	WBGene00001574. gei-17.
WormPep	W10D5.3a. CE14782. [WorfDB] W10D5.3c. CE34390. [WorfDB] W10D5.3d. CE24047. [WorfDB] W10D5.3e. CE34391. [WorfDB] W10D5.3f. CE34392. [WorfDB] W10D5.3g. CE38434. [WorfDB]
Gene expression databases
ArrayExpress	Q94361.
Family and domain databases
InterPro	IPR004181. Znf_MIZ. [Graphical view]
Pfam	PF02891. zf-MIZ. 1 hit. [Graphical view]
PROSITE	PS51044. ZF_SP_RING. 1 hit. [Graphical view]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

GEI17_CAEEL

Accession

Primary (citable) accession number: Q94361
Secondary accession number(s): Q564X2 , Q7Z0X3, Q7Z0X4, Q94363, Q9U326

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	October 1, 2003
Last modified:	July 22, 2008
This is version 50 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project