Reviewed, UniProtKB/Swiss-Prot Q94BT6 (ADO1_ARATH)

Names and origin

Protein names

Recommended name:
    Adagio protein 1
Alternative name(s):
    Protein ZEITLUPE
    Clock-associated PAS protein ZTL
    LOV kelch protein 1
    Flavin-binding kelch repeat F-box protein 1-like protein 2
      Short name(s)=FKF1-like protein 2
    F-box only protein 2b
      Short name(s)=FBX2b

Gene names

Name:	ADO1
Synonyms:	FKL2, LKP1, ZTL
Ordered Locus Names:	At5g57360
ORF Names:	MSF19.2

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	609 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of E3 ubiquitin ligase complexes that plays a central role blue-light-dependent circadian cycles. Acts as a blue-light photoreceptor, due to the presence of FMN, that mediates light-regulated protein degradation of critical clock components by targeting them to the proteasome complex. The SCF(ADO1) E3 ubiquitin ligase complex is involved in the regulation of circadian clock-dependent processes including transition to flowering time, hypocotyl elongation, cotyledons and leaves movement rhythms. APRR1 and APRR5 seem to be substrates of the SCF(ADO1) complex.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with ADO2. Component of an E3 ubiquitin ligase SCF(ADO1) complex composed of SKP1A/ASK1 (or SKP1B/ASK2), CUL1, RBX1 and ADO1. Also interacts with SKP1D/ASK4, SKP1K/ASK11, CRY1, PHYB, APRR1 and APRR5, and probably with SKP1N/ASK14 and SKP1S/ASK19.
Subcellular location	Nucleus. Cytoplasm. Note= Nuclear after 9 hours of illumination (afternoon of long days). Cytoplasmic when plant have been subsequently grown 16 hours in light and 5 hours in dark (early morning of long days).
Tissue specificity	Ubiquitously expressed with higher levels in cotyledons and leaves.
Developmental stage	Mainly present during the light phase, and degraded in a proteasome-dependent manner in dark (at protein level).
Post-translational modification	May be ubiquitinated. Degraded in a proteasome-dependent manner. FMN binds covalently to cysteine after exposure to blue light and is reversed in the dark By similarity.
Miscellaneous	'Zeitlupe' means slow motion in German. 'Adagio' means slowly in Italian.
Sequence similarities	Belongs to the ADAGIO family. Contains 1 F-box domain. Contains 5 Kelch repeats. Contains 1 PAC (PAS-associated C-terminal) domain. Contains 1 PAS (PER-ARNT-SIM) domain.
Sequence caution	AAK64006.1 sequence differs from that shown. Reason: Frameshift at positions 462 and 467.

Ontologies

Keywords
Biological process	Biological rhythms Sensory transduction Ubl conjugation pathway
Cellular component	Cytoplasm Nucleus
Domain	Kelch repeat Repeat
Ligand	Chromophore FMN Flavoprotein
Molecular function	Photoreceptor protein Receptor
PTM	Ubl conjugation
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	protein binding Ref.4 Ref.8 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
APRR1	Q9LKL2	4	EBI-300691,EBI-618423
AT4g34210	O49484	2	EBI-300691,EBI-401185
CRY1	Q43125	1	EBI-300691,EBI-300703
GI	Q9SQI2	4	EBI-300691,EBI-446380
PHYB	P14713	1	EBI-300691,EBI-300727
SKP1A	Q39255	4	EBI-300691,EBI-532357
SKP1B	Q9FHW7	2	EBI-300691,EBI-604076

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 609	609	Adagio protein 1
Regions
	Domain	32 – 114	83	PAS
	Domain	118 – 161	44	PAC
	Domain	195 – 241	47	F-box
	Repeat	292 – 342	51	Kelch 1
	Repeat	345 – 392	48	Kelch 2
	Repeat	397 – 445	49	Kelch 3
	Repeat	450 – 501	52	Kelch 4
	Repeat	516 – 564	49	Kelch 5
Amino acid modifications
	Modified residue	82	1	S-4a-FMN cysteine By similarity
Experimental info
	Mutagenesis	200	1	L → A: No SCF(ADO1) complex formation and reduced cyclic degradation of ADO1; when associated with A-213
	Mutagenesis	213	1	L → A: No SCF(ADO1) complex formation and reduced cyclic degradation of ADO1; when associated with A-200
	Mutagenesis	320	1	D → N in ztl-2; affects circadian clock by lengthening the free-running period of clock-controlled processes
	Mutagenesis	425	1	D → N in ztl-1; affects circadian clock by lengthening the free-running period of clock-controlled processes

Sequences

	Sequence		Length	Mass (Da)
	Q94BT6-1 [UniParc]. Last modified November 8, 2005. Version 2. Checksum: B25192FCBE019093	FASTA	609	65,906
	10 20 30 40 50 60 MEWDSGSDLS ADDASSLADD EEGGLFPGGG PIPYPVGNLL HTAPCGFVVT DAVEPDQPII 70 80 90 100 110 120 YVNTVFEMVT GYRAEEVLGG NCRFLQCRGP FAKRRHPLVD SMVVSEIRKC IDEGIEFQGE 130 140 150 160 170 180 LLNFRKDGSP LMNRLRLTPI YGDDDTITHI IGIQFFIETD IDLGPVLGSS TKEKSIDGIY 190 200 210 220 230 240 SALAAGERNV SRGMCGLFQL SDEVVSMKIL SRLTPRDVAS VSSVCRRLYV LTKNEDLWRR 250 260 270 280 290 300 VCQNAWGSET TRVLETVPGA KRLGWGRLAR ELTTLEAAAW RKLSVGGSVE PSRCNFSACA 310 320 330 340 350 360 VGNRVVLFGG EGVNMQPMND TFVLDLNSDY PEWQHVKVSS PPPGRWGHTL TCVNGSNLVV 370 380 390 400 410 420 FGGCGQQGLL NDVFVLNLDA KPPTWREISG LAPPLPRSWH SSCTLDGTKL IVSGGCADSG 430 440 450 460 470 480 VLLSDTFLLD LSIEKPVWRE IPAAWTPPSR LGHTLSVYGG RKILMFGGLA KSGPLKFRSS 490 500 510 520 530 540 DVFTMDLSEE EPCWRCVTGS GMPGAGNPGG VAPPPRLDHV AVNLPGGRIL IFGGSVAGLH 550 560 570 580 590 600 SASQLYLLDP TEDKPTWRIL NIPGRPPRFA WGHGTCVVGG TRAIVLGGQT GEEWMLSELH ELSLASYLT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"ZEITLUPE encodes a novel clock-associated PAS protein from Arabidopsis." Somers D.E., Schultz T.F., Milnamow M., Kay S.A. Cell 101:319-329(2000) [PubMed: 10847686] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION, MUTAGENESIS OF ASP-320 AND ASP-425.
[2]	"FKF1, a clock-controlled gene that regulates the transition to flowering in Arabidopsis." Nelson D.C., Lasswell J.E., Rogg L.E., Cohen M.A., Bartel B. Cell 101:331-340(2000) [PubMed: 10847687] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
[3]	"LKP1 (LOV kelch protein 1): a factor involved in the regulation of flowering time in Arabidopsis." Kiyosue T., Wada M. Plant J. 23:807-815(2000) [PubMed: 10998191] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INDUCTION.
[4]	"An Arabidopsis circadian clock component interacts with both CRY1 and phyB." Jarillo J.A., Capel J., Tang R.-H., Yang H.-Q., Alonso J.M., Ecker J.R., Cashmore A.R. Nature 410:487-490(2001) [PubMed: 11260718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH CRY1 AND PHYB.
[5]	"Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence features of the regions of 1,081,958 bp covered by seventeen physically assigned P1 and TAC clones." Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N., Tabata S. DNA Res. 5:379-391(1998) [PubMed: 10048488] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[6]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[7]	"F-box proteins in Arabidopsis." Xiao W., Jang J.-C. Trends Plant Sci. 5:454-457(2000) [PubMed: 11077244] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[8]	"Protein interaction analysis of SCF ubiquitin E3 ligase subunits from Arabidopsis." Risseeuw E.P., Daskalchuk T.E., Banks T.W., Liu E., Cotelesage J., Hellmann H., Estelle M., Somers D.E., Crosby W.L. Plant J. 34:753-767(2003) [PubMed: 12795696] [Abstract] Cited for: INTERACTION WITH SKP1A; SKP1B; SKP1D; SKP1K AND SKP1S.
[9]	"Circadian phase-specific degradation of the F-box protein ZTL is mediated by the proteasome." Kim W.-Y., Geng R., Somers D.E. Proc. Natl. Acad. Sci. U.S.A. 100:4933-4938(2003) [PubMed: 12665620] [Abstract] Cited for: INDUCTION.
[10]	"Identification of ASK and clock-associated proteins as molecular partners of LKP2 (LOV kelch protein 2) in Arabidopsis." Yasuhara M., Mitsui S., Hirano H., Takanabe R., Tokioka Y., Ihara N., Komatsu A., Seki M., Shinozaki K., Kiyosue T. J. Exp. Bot. 55:2015-2027(2004) [PubMed: 15310821] [Abstract] Cited for: INTERACTION WITH SKP1A; SKP1B; SKP1D; SKP1K; SKP1N; ADO2; APRR1 AND APRR5.
[11]	"The F-box protein ZEITLUPE confers dosage-dependent control on the circadian clock, photomorphogenesis, and flowering time." Somers D.E., Kim W.-Y., Geng R. Plant Cell 16:769-782(2004) [PubMed: 14973171] [Abstract] Cited for: FUNCTION.
[12]	"Formation of an SCF(ZTL) complex is required for proper regulation of circadian timing." Han L., Mason M., Risseeuw E.P., Crosby W.L., Somers D.E. Plant J. 40:291-301(2004) [PubMed: 15447654] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF LEU-200 AND LEU-213, IDENTIFICATION IN A SCF(ADO1) COMPLEX.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF254413 mRNA. Translation: AAF70288.1. AF216525 mRNA. Translation: AAF32300.1. AB038796 mRNA. Translation: BAB18914.1. AF252294 mRNA. Translation: AAK27433.1. AB016891 Genomic DNA. Translation: BAB08473.1. AY039902 mRNA. Translation: AAK64006.1. Frameshift. BT008772 mRNA. Translation: AAP68211.1.
RefSeq	NP_568855.1.
UniGene	At.21022
3D structure databases
HSSP	HSSP built from PDB template 1N9L based on UniProtKB Q8LPE0.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q94BT6.
Genome annotation databases
GeneID	835842.
GenomeReviews	Gene locus AT5G57360 in contig BA000015_GR.
KEGG	ath:AT5G57360.
NMPDR	fig|3702.1.peg.27688.
Organism-specific databases
GeneFarm	5106. 485.
TAIR	At5g57360.
Gene expression databases
GermOnline	AT5G57360. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR001810. F-box. IPR015915. Kelch-typ_b-propeller. IPR006652. Kelch_1. IPR011498. Kelch_2. IPR001610. PAC. IPR000014. PAS. IPR000700. PAS-assoc_C. [Graphical view]
Gene3D	G3DSA:2.120.10.80. Kelch-typ_b-propeller. 1 hit.
Pfam	PF00646. F-box. 1 hit. PF01344. Kelch_1. 1 hit. PF07646. Kelch_2. 4 hits. [Graphical view]
SMART	SM00256. FBOX. 1 hit. SM00086. PAC. 1 hit. SM00091. PAS. 1 hit. [Graphical view]
PROSITE	PS50181. FBOX. 1 hit. PS50113. PAC. False negative. PS50112. PAS. 1 hit. [Graphical view]
ProDom	Q94BT6. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

ADO1_ARATH

Accession

Primary (citable) accession number: Q94BT6
Secondary accession number(s): Q9LDF6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
Last sequence update:	November 8, 2005
Last modified:	July 22, 2008
This is version 48 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)